scholarly journals Normal mammary epithelial cells promote carcinoma basement membrane invasion by inducing microtubule-rich protrusions

Oncotarget ◽  
2015 ◽  
Vol 6 (32) ◽  
pp. 32634-32645 ◽  
Author(s):  
Meng-Horng Lee ◽  
Pei-Hsun Wu ◽  
Daniele Gilkes ◽  
Ivie Aifuwa ◽  
Denis Wirtz
Keyword(s):  
Epithelial Cells ◽  
Basement Membrane ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Normal Mammary Epithelial

The role of laminin-5 and its receptors in mammary epithelial cell branching morphogenesis

1997 ◽  
Vol 110 (1) ◽  
pp. 55-63 ◽  
Author(s):  
S. Stahl ◽  
S. Weitzman ◽  
J.C. Jones
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Branching Morphogenesis ◽  
Mammary Epithelial ◽  
Breast Epithelial Cell ◽  
Epithelial Tissue ◽  
Laminin 5 ◽  
Normal Mammary Epithelial

In vivo, normal mammary epithelial cells utilize hemidesmosome attachment devices to adhere to stroma. However, analyses of a potential role for hemidesmosomes and their components in mammary epithelial tissue morphogenesis have never been attempted. MCF-10A cells are a spontaneously immortalized line derived from mammary epithelium and possess a number of characteristics of normal mammary epithelial cells including expression of hemidesmosomal associated proteins such as the two bullous pemphigoid antigens, alpha 6 beta 4 integrin and its ligand laminin-5. More importantly, MCF-10A cells readily assemble mature hemidesmosomes when plated onto uncoated substrates. When maintained on matrigel, like their normal breast epithelial cell counterparts, MCF-10A cells undergo a branching morphogenesis and assemble hemidesmosomes at sites of cell-matrigel interaction. Function blocking antibodies specific for human laminin-5 and the alpha subunits of its two known receptors (alpha 3 beta 1 and alpha 6 beta 4 integrin) not only inhibit hemidesmosome assembly by MCF-10A cells but also impede branching morphogenesis induced by matrigel. Our results imply that the hemidesmosome, in particular those subunits comprising its laminin-5/integrin ‘backbone’, play an important role in morphogenetic events. We discuss these results in light of recent evidence that hemidesmosomes are sites involved in signal transduction.

scholarly journals Plasticity of patient-matched normal mammary epithelial cells is dependent on autologous adipose-derived stem cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Annika Kengelbach-Weigand ◽  
Kereshmeh Tasbihi ◽  
Pamela L. Strissel ◽  
Rafael Schmid ◽  
Jasmin Monteiro Marques ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Adipose Derived Stem Cells ◽  
Normal Mammary Epithelial

Physical Connections between Feeder Cells and Recipient Normal Mammary Epithelial Cells

1998 ◽  
Vol 243 (1) ◽  
pp. 76-86 ◽  
Author(s):  
Ursula K. Ehmann ◽  
Mary Ann Stevenson ◽  
Stuart K. Calderwood ◽  
James T. DeVries
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Feeder Cells ◽  
Normal Mammary Epithelial
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