scholarly journals Complementary genetic screens identify the E3 ubiquitin ligase CBLC, as a modifier of PARP inhibitor sensitivity

Oncotarget ◽  
2015 ◽  
Vol 6 (13) ◽  
pp. 10746-10758 ◽  
Author(s):  
Jessica Frankum ◽  
Pavel Moudry ◽  
Rachel Brough ◽  
Zdenek Hodny ◽  
Alan Ashworth ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
Parp Inhibitor ◽  
E3 Ubiquitin Ligase ◽  
Genetic Screens

scholarly journals The E3 ubiquitin ligase adaptor Tango10 links the core circadian clock to neuropeptide and behavioral rhythms

2021 ◽  
Vol 118 (47) ◽  
pp. e2110767118
Author(s):  
Jongbin Lee ◽  
Chunghun Lim ◽  
Tae Hee Han ◽  
Tomas Andreani ◽  
Matthew Moye ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Genetic Screens ◽  
Pacemaker Neurons ◽  
Behavioral Rhythms ◽  
Pigment Dispersing Factor ◽  
The Core ◽  
Cullin 3 ◽  
Patch Clamp Electrophysiology ◽  
Golgi Organization

Circadian transcriptional timekeepers in pacemaker neurons drive profound daily rhythms in sleep and wake. Here we reveal a molecular pathway that links core transcriptional oscillators to neuronal and behavioral rhythms. Using two independent genetic screens, we identified mutants of Transport and Golgi organization 10 (Tango10) with poor behavioral rhythmicity. Tango10 expression in pacemaker neurons expressing the neuropeptide PIGMENT-DISPERSING FACTOR (PDF) is required for robust rhythms. Loss of Tango10 results in elevated PDF accumulation in nerve terminals even in mutants lacking a functional core clock. TANGO10 protein itself is rhythmically expressed in PDF terminals. Mass spectrometry of TANGO10 complexes reveals interactions with the E3 ubiquitin ligase CULLIN 3 (CUL3). CUL3 depletion phenocopies Tango10 mutant effects on PDF even in the absence of the core clock gene timeless. Patch clamp electrophysiology in Tango10 mutant neurons demonstrates elevated spontaneous firing potentially due to reduced voltage-gated Shaker-like potassium currents. We propose that Tango10/Cul3 transduces molecular oscillations from the core clock to neuropeptide release important for behavioral rhythms.

Author response for "TAM receptors attenuate murine NK cell responses via E3 ubiquitin ligase Cbl‐b"

2019 ◽  
Author(s):  
Leilani M. Chirino ◽  
Suresh Kumar ◽  
Mariko Okumura ◽  
David E. Sterner ◽  
Michael Mattern ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
Nk Cell ◽  
E3 Ubiquitin Ligase ◽  
Author Response ◽  
Cell Responses ◽  
Tam Receptors
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