scholarly journals CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid

Oncotarget ◽  
2015 ◽  
Vol 6 (8) ◽  
pp. 5889-5902 ◽  
Author(s):  
Shi-Lin Xia ◽  
Mo-Li Wu ◽  
Hong Li ◽  
Jia-Hui Wang ◽  
Nan-Nan Chen ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Human Glioblastoma Cells ◽  
Crabp Ii

scholarly journals Differential effects of 9-cis and all-trans retinoic acid on the induction of retinoic acid receptor-β and cellular retinoic acid-binding protein II in human neuroblastoma cells

1994 ◽  
Vol 304 (1) ◽  
pp. 147-154 ◽  
Author(s):  
C P F Redfern ◽  
P E Lovat ◽  
A J Malcolm ◽  
A D J Pearson
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Mrna Levels ◽  
Human Neuroblastoma ◽  
Acid Receptor ◽  
Acid Binding Protein ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
High Concentrations ◽  
Crabp Ii

The objective of this study was to compare the properties of 9-cis and all-trans retinoic acid with respect to the induction of expression of retinoic acid receptor beta (RAR-beta) and cellular retinoic acid-binding protein (CRABP) II in human neuroblastoma SH SY 5Y cells. RAR-beta and CRABP II mRNA was induced by both all-trans and 9-cis retinoic acid in SH SY 5Y cells. Induction was rapid, detectable within 2-4 h, and inhibited by actinomycin D. Time-courses of induction for RAR-beta and CRABP II differed: RAR-beta mRNA levels reached a maximum 4-6 h after adding all-trans or 9-cis retinoic acid, whereas CRABP II mRNA levels increased over at least 18 h. These differences were attributed to the longer half-life of CRABP II mRNA (20 h) compared with RAR-beta mRNA (3.9 h). The dose-response characteristics of all-trans and 9-cis retinoic acid were different: all-trans was effective at nanomolar concentrations, whereas 10-fold higher levels of 9-cis retinoic acid were required to achieve comparable induction of RAR-beta and CRABP II. Conversely, at high concentrations, 9-cis retinoic acid gave a greater induction of RAR-beta and CRABP II than all-trans. The induction of RAR-beta and CRABP II by all-trans retinoic acid was maintained in the subsequent absence of all-trans retinoic acid, whereas induction by 9-cis retinoic acid was dependent on its continued presence in the culture medium. These results suggest that, at high concentrations, 9-cis retinoic acid may produce its transcriptional effects via retinoid X receptor (RXR) homodimers. This has implications for the cellular functions of 9-cis retinoic acid and its use as a biological response modifier.

scholarly journals All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells

2017 ◽  
Vol 69 (4) ◽  
pp. 699-706
Author(s):  
Jelena Marjanovic-Vicentic ◽  
Marija Schwirtlich ◽  
Natasa Kovacevic-Grujicic ◽  
Milena Stevanovic ◽  
Danijela Drakulic
Keyword(s):  
Retinoic Acid ◽  
Cell Morphology ◽  
Neural Differentiation ◽  
Glioblastoma Cells ◽  
Atra Treatment ◽  
Cell Matrix ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Cell Matrix Adhesion ◽  
U251 Cells

Glioblastoma (GBM) is one of the most aggressive and deadly forms of cancer. Literature data reveals that all-trans retinoic acid (ATRA) has anticancer effects on different types of tumor cells. However, data about the effects of ATRA on glioblastoma cells are contradictory. In this study, we examined whether ATRA treatment affects features of human glioblastoma U251 cells. To that end, the cells were treated with different concentrations of ATRA. Results obtained by MTT and the crystal violet assays imply that ATRA affected the viability of U251 glioblastoma cells in a dose- and time-dependent manner. Fluorescence staining of microtubule cytoskeleton protein ?-tubulin revealed that ATRA induced changes in cell morphology. Using semi-quantitative RT-PCR we found that the expression of SOX3 and GFAP genes, as markers of neural differentiation, was not changed upon ATRA treatment. Thus, the observed changes in cell morphology after ATRA treatment are not associated with neural differentiation of U251 glioblastoma cells. The scratch-wound healing assay revealed that ATRA changed the mode of U251 cell migration from collective to single cell motility. The cell-matrix adhesion assay demonstrated that the pharmacologically relevant concentration of ATRA lowered the cell-matrix adhesion capability of U251 cells. In conclusion, our results imply that further studies are needed before ATRA could be considered for the treatment of glioblastoma.

Comparison of CD271 (Adapalene) and All-Trans Retinoic Acid in Human Skin: Dissociation of Epidermal Effects and CRABP-II mRNA Expression

1993 ◽  
Vol 101 (3) ◽  
pp. 325-328 ◽  
Author(s):  
Christopher E M Griffiths ◽  
James T Elder ◽  
Bruno A Bernard ◽  
Patricia Rossio ◽  
Matthew A Cromie ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Mrna Expression ◽  
Human Skin ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Crabp Ii

Antiproliferative effect of all-trans-retinoic acid in cholangiocarcinoma

2017 ◽  
Author(s):  
A Prawan ◽  
S Butsri ◽  
V Kukongviriyapan ◽  
L Senggunprai ◽  
S Kongpetch
Keyword(s):  
Retinoic Acid ◽  
Antiproliferative Effect ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid
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