scholarly journals Autophagy through 4EBP1 and AMPK regulates oxidative stress-induced premature senescence in auditory cells

Oncotarget ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 3644-3655 ◽  
Author(s):  
Nana Akagi Tsuchihashi ◽  
Ken Hayashi ◽  
Katsuaki Dan ◽  
Fumiyuki Goto ◽  
Yasuyuki Nomura ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Premature Senescence ◽  
Stress Induced Premature Senescence

scholarly journals Role of p16INK4a and BMI-1 in oxidative stress-induced premature senescence in human dental pulp stem cells

Redox Biology ◽  
2017 ◽  
Vol 12 ◽  
pp. 690-698 ◽  
Author(s):  
Cristina Mas-Bargues ◽  
José Viña-Almunia ◽  
Marta Inglés ◽  
Jorge Sanz-Ros ◽  
Juan Gambini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Premature Senescence ◽  
Human Dental Pulp ◽  
Stress Induced Premature Senescence

scholarly journals DRG2 Accelerates Senescence via Negative Regulation of SIRT1 in Human Diploid Fibroblasts

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Bing Si Li ◽  
Ai Lin Jin ◽  
ZiQi Zhou ◽  
Jae Ho Seo ◽  
Byung-Min Choi
Keyword(s):  
Oxidative Stress ◽  
Cell Growth ◽  
Binding Protein ◽  
Ectopic Expression ◽  
Sirtuin 1 ◽  
Premature Senescence ◽  
Gtp Binding Protein ◽  
Human Diploid Fibroblasts ◽  
Gtp Binding ◽  
Stress Induced Premature Senescence

Accumulating evidence suggests that developmentally regulated GTP-binding protein 2 (DRG2), an evolutionarily conserved GTP-binding protein, plays an important role in regulating cell growth, inflammation, and mitochondria dynamics. However, the effect of DRG2 in aging remains unclear. In this study, we found that endogenous DRG2 protein expression is upregulated in oxidative stress-induced premature senescence models and tissues of aged mice. Ectopic expression of DRG2 significantly promoted senescence-associated β-galactosidase (SA-β-gal) activity and inhibited cell growth, concomitant with increase in levels of acetyl (ac)-p53 (Lys382), ac-nuclear factor-kB (NF-κB) p65 (Lys310), p21Waf1/Cip1, and p16Ink4a and a decrease in cyclin D1. In this process, reactive oxygen species (ROS) and phosphorylation of H2A histone family member X (H2A.X), forming γ-H2A.X, were enhanced. Mechanistically, ectopic expression of DRG2 downregulated Sirtuin-1 (SIRT1), resulting in augmented acetylation of p53 and NF-κB p65. Additionally, DRG2 knockdown significantly abolished oxidative stress-induced premature senescence. Our results provide a possible molecular mechanism for investigation of cellular senescence and aging regulated by DRG2.

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