scholarly journals Chimeric chromosome landscapes of human somatic cell cultures show dependence on stress and regulation of genomic repeats by CGGBP1

Oncotarget ◽  
2021 ◽  
Author(s):  
Subhamoy Datta ◽  
Manthan Patel ◽  
Sukesh Kashyap ◽  
Divyesh Patel ◽  
Umashankar Singh
Keyword(s):  
Somatic Cell ◽  
Cell Cultures ◽  
Human Somatic Cell ◽  
Genomic Repeats

scholarly journals Chimeric chromosome landscapes of human somatic cell cultures show dependence on stress and regulation of genomic repeats by CGGBP1.

2021 ◽  
Author(s):  
Subhamoy Datta ◽  
Manthan Patel ◽  
Sukesh Kashyap ◽  
Divyesh Patel ◽  
Umashankar Singh
Keyword(s):  
Somatic Cell ◽  
Cell Cultures ◽  
Cultured Cells ◽  
De Novo ◽  
Human Fibroblasts ◽  
Regulatory Protein ◽  
Somatic Mosaicism ◽  
Experimental Conditions ◽  
Significant Information ◽  
Genomic Repeats

Genomes of somatic cells in culture are prone to spontaneous mutations due to errors in replication and DNA repair. Some of these errors, such as chromosomal fusions, are not rectifiable and subject to selection or elimination in growing cultures. Somatic cell cultures are thus expected to generate background levels of potentially stable chromosomal chimeras. A description of the landscape of such spontaneously generated chromosomal chimeras in cultured cells will help us understand the factors affecting somatic mosaicism. Here we show that short homology-associated non-homologous chromosomal chimeras occur in normal human fibroblasts and HEK293T cells at genomic repeats. The occurrence of chromosomal chimeras is enhanced by heat stress and depletion of a repeat regulatory protein CGGBP1. We also present evidence of homologous chromosomal chimeras between allelic copies in repeat-rich DNA obtained by methylcytosine immunoprecipitation. The formation of homologous chromosomal chimeras at Alu and L1 repeats increases upon depletion of CGGBP1. Our data are derived from de novo sequencing from three different cell lines under different experimental conditions and our chromosomal chimera detection pipeline is applicable to long read as well as short read sequencing platforms. These findings present significant information about the generation, sensitivity and regulation of somatic mosaicism in human cell cultures.

scholarly journals Guido Pontecorvo. 29 November 1907 – 25 September 1999

2002 ◽  
Vol 48 ◽  
pp. 375-390 ◽  
Author(s):  
O. Siddiqi
Keyword(s):  
Somatic Cell ◽  
Aspergillus Nidulans ◽  
Royal Society ◽  
Large Body ◽  
Somatic Cell Genetics ◽  
Parasexual Cycle ◽  
Sense Of Humor ◽  
Human Somatic Cell

Guido Pontecorvo was elected a Fellow of The Royal Society in 1955 for his contributions to the genetics of Drosophila and the fungus Aspergillus nidulans . Pontecorvo was a leading British geneticist, prominent in the decade preceding the discovery of DNA, who enriched our understanding of genes and whose pioneering work on the parasexual cycle in fungi found application in human somatic cell genetics. Known to friends as Ponte, he had a strong personality. Somewhat irascible but warm, with a wry sense of humor, he made many lifelong friends and acquired a large body of admirers.

MUTAGENIC RESPONSE OF HUMAN SOMATIC CELL LINES

1964 ◽  
pp. 209-221
Author(s):  
WACLAW SZYBALSKI ◽  
G. RAGNI ◽  
NAOMI K. COHN
Keyword(s):  
Somatic Cell ◽  
Cell Lines ◽  
Human Somatic Cell ◽  
Mutagenic Response
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