scholarly journals Redaporfin induces immunogenic cell death by selective destruction of the endoplasmic reticulum and the Golgi apparatus

Oncotarget ◽  
2018 ◽  
Vol 9 (58) ◽  
pp. 31169-31170 ◽  
Author(s):  
Lígia Catarina Gomes-da-Silva ◽  
Liwei Zhao ◽  
Luis G. Arnaut ◽  
Guido Kroemer ◽  
Oliver Kepp
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Golgi Apparatus ◽  
Immunogenic Cell Death ◽  
Selective Destruction

scholarly journals Immunogenic Cell Death of Breast Cancer Stem Cells Induced by an Endoplasmic Reticulum‐Targeting Copper(II) Complex

ChemBioChem ◽  
2020 ◽  
Vol 21 (24) ◽  
pp. 3618-3624 ◽  
Author(s):  
Pooja Kaur ◽  
Alice Johnson ◽  
Joshua Northcote‐Smith ◽  
Chunxin Lu ◽  
Kogularamanan Suntharalingam
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Cancer Stem Cells ◽  
Immunogenic Cell Death ◽  
Breast Cancer Stem Cells ◽  
Endoplasmic Reticulum Targeting

scholarly journals Synthetic induction of immunogenic cell death by genetic stimulation of endoplasmic reticulum stress

2014 ◽  
Vol 3 (3) ◽  
pp. e28276 ◽  
Author(s):  
Mickaël Michaud ◽  
Abdul Qader Sukkurwala ◽  
Federica Di Sano ◽  
Laurence Zitvogel ◽  
Oliver Kepp ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Endoplasmic Reticulum Stress ◽  
Immunogenic Cell Death ◽  
Stimulation Of

Endoplasmic Reticulum Targeting to Amplify Immunogenic Cell Death for Cancer Immunotherapy

Nano Letters ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 1928-1933 ◽  
Author(s):  
Hongzhang Deng ◽  
Zijian Zhou ◽  
Weijing Yang ◽  
Li-sen Lin ◽  
Sheng Wang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Cancer Immunotherapy ◽  
Immunogenic Cell Death ◽  
Endoplasmic Reticulum Targeting

scholarly journals Bullatacin triggers immunogenic cell death of colon cancer cells by activating endoplasmic reticulum chaperones

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Fangtian Fan ◽  
Peiliang Shen ◽  
Yue Ma ◽  
Wangbo Ma ◽  
Hongyan Wu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Western Blot ◽  
Antitumour Activity ◽  
Cancer Drug ◽  
Immunogenic Cell Death ◽  
Dependent Manner ◽  
Macrophage Phagocytosis ◽  
Ht 29

Abstract Background It is well accepted that the immune system efficiently contributes to positive outcomes of chemotherapeutic cancer treatment by activating immunogenic cell death (ICD). However, only a limited number of ICD-inducing compounds are well characterized at present; therefore, identification of novel ICD inducers is urgently needed for cancer drug discovery, and the need is becoming increasingly urgent. Methods Herein, we assessed the antitumour activity of bullatacin by MTS assay and apoptosis assay. ICD biomarkers, such as calreticulin (CRT), high-mobility group protein B1 (HMGB-1), heat shock protein (HSP)70, HSP90 and ATP, were assessed by Western blotting, ELISA and flow cytometry. Western blot and qPCR assays were performed to explore the underlying mechanisms of bullatacin-induced ICD. Flow cytometry was used to detect macrophage phagocytosis. Results First, bullatacin induced apoptosis in both SW480 cells and HT-29 cells in a time-dependent manner at 10 nM, as assessed by flow cytometry. Moreover, Western blot and flow cytometry assays showed that CRT and HSP90 (biomarkers of early ICD) significantly accumulated on the cell membrane surface after approximately 6 h of treatment with bullatacin. In addition, ELISAs and Western blot assays showed that the second set of hallmarks required for ICD (HMGB1, HSP70 and HSP90) were released in the conditioned media of both SW480 and HT-29 cells after 36 h of treatment. Furthermore, qPCR and Western blot assays indicated that bullatacin triggered ICD via activation of the endoplasmic reticulum stress (ERS) signalling pathway. Finally, bullatacin promoted macrophage phagocytosis. Conclusion This study documents that bullatacin, a novel ICD inducer, triggers immunogenic tumour cell death by activating ERS even at a relatively low concentration in vitro.

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