scholarly journals Galectin-3 sensitized melanoma cell lines to vemurafenib (PLX4032) induced cell death through prevention of autophagy

Oncotarget ◽  
2018 ◽  
Vol 9 (18) ◽  
pp. 14567-14579 ◽  
Author(s):  
Silvina Odete Bustos ◽  
Gustavo José da Silva Pereira ◽  
Renata de Freitas Saito ◽  
Cristiane Damas Gil ◽  
Daniela Bertolli Zanatta ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Galectin 3 ◽  
Melanoma Cell Lines

scholarly journals Photodynamic treatment induces cell death by apoptosis or autophagy depending on the melanin content in two B16 melanoma cell lines

2012 ◽  
Vol 29 (3) ◽  
pp. 1196-1200 ◽  
Author(s):  
AGNÈS SPARSA ◽  
SOLENN BELLATON ◽  
THOMAS NAVES ◽  
MARIE-ODILE JAUBERTEAU ◽  
JEAN-MARIE BONNETBLANC ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Melanoma Cell ◽  
B16 Melanoma ◽  
Melanin Content ◽  
Photodynamic Treatment ◽  
B16 Melanoma Cell ◽  
Melanoma Cell Lines

scholarly journals The combination of ADI-PEG20 and TRAIL effectively increases cell death in melanoma cell lines

2010 ◽  
Vol 394 (3) ◽  
pp. 760-766 ◽  
Author(s):  
Min You ◽  
Niramol Savaraj ◽  
Medhi Wangpaichitr ◽  
Chunjing Wu ◽  
M. Tien Kuo ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Melanoma Cell Lines

scholarly journals Dual mTOR/DNA-PK Inhibitor CC-115 Induces Cell Death in Melanoma Cells and Has Radiosensitizing Potential

2020 ◽  
Vol 21 (23) ◽  
pp. 9321
Author(s):  
Felix Bürkel ◽  
Tina Jost ◽  
Markus Hecht ◽  
Lucie Heinzerling ◽  
Rainer Fietkau ◽  
...  
Keyword(s):  
Cell Death ◽  
Metastatic Melanoma ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Fibroblast Cell ◽  
Malignant Cell ◽  
Cell Cycle Distribution ◽  
Dual Inhibitor ◽  
Donor Skin ◽  
Melanoma Cell Lines

CC-115 is a dual inhibitor of the mechanistic target of rapamycin (mTOR) kinase and the DNA-dependent protein kinase (DNA-PK) that is currently being studied in phase I/II clinical trials. DNA-PK is essential for the repair of DNA-double strand breaks (DSB). Radiotherapy is frequently used in the palliative treatment of metastatic melanoma patients and induces DSBs. Melanoma cell lines and healthy-donor skin fibroblast cell lines were treated with CC-115 and ionizing irradiation (IR). Apoptosis, necrosis, and cell cycle distribution were analyzed. Colony forming assays were conducted to study radiosensitizing effects. Immunofluorescence microscopy was performed to determine the activity of homologous recombination (HR). In most of the malign cell lines, an increasing concentration of CC-115 resulted in increased cell death. Furthermore, strong cytotoxic effects were only observed in malignant cell lines. Regarding clonogenicity, all cell lines displayed decreased survival fractions during combined inhibitor and IR treatment and supra-additive effects of the combination were observable in 5 out of 9 melanoma cell lines. CC-115 showed radiosensitizing potential in 7 out of 9 melanoma cell lines, but not in healthy skin fibroblasts. Based on our data CC-115 treatment could be a promising approach for patients with metastatic melanoma, particularly in the combination with radiotherapy.

scholarly journals G3139 induces cell death by caspase-dependent and -independent apoptosis on human melanoma cell lines

2006 ◽  
Author(s):  
Shinnosuke Nakamura ◽  
Madoka Inui ◽  
Takahiko Kamei ◽  
Minoru Nakase ◽  
Kenya Okumura ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Human Melanoma ◽  
Human Melanoma Cell ◽  
Melanoma Cell Lines

scholarly journals Selective Induction of Cell Death in Melanoma Cell Lines through Targeting of Mcl-1 and A1

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e30821 ◽  
Author(s):  
Daniela Senft ◽  
Carola Berking ◽  
Saskia A. Graf ◽  
Claudia Kammerbauer ◽  
Thomas Ruzicka ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Melanoma Cell Lines

scholarly journals Cytotoxic Effect of Vanicosides A and B from Reynoutria sachalinensis against Melanotic and Amelanotic Melanoma Cell Lines and in silico Evaluation for Inhibition of BRAFV600E and MEK1

2020 ◽  
Vol 21 (13) ◽  
pp. 4611 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Anna Choromańska ◽  
Renata Abel ◽  
Robert Preissner ◽  
Jolanta Saczko ◽  
...  
Keyword(s):  
Cell Death ◽  
Molecular Docking ◽  
Cell Line ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Primary Fibroblast ◽  
Fibroblast Line ◽  
Reynoutria Sachalinensis ◽  
A375 Cell Line ◽  
Melanoma Cell Lines

Vanicosides A and B are the esters of hydroxycinnamic acids with sucrose, occurring in a few plant species from the Polygonaceae family. So far, vanicosides A and B have not been evaluated for anticancer activity against human malignant melanoma. In this study, we tested these two natural products, isolated from Reynoutria sachalinensis rhizomes, against two human melanoma cell lines (amelanotic C32 cell line and melanotic A375 cell line, both bearing endogenous BRAFV600E mutation) and two normal human cell lines—keratinocytes (HaCaT) and the primary fibroblast line. Additionally, a molecular docking of vanicoside A and vanicoside B with selected targets involved in melanoma progression was performed. Cell viability was studied using an MTT assay. A RealTime-Glo™ Annexin V Apoptosis and Necrosis assay was used for monitoring programmed cell death (PCD). Vanicoside A demonstrated strong cytotoxicity against the amelanotic C32 cell line (viability of the C32 cell line was decreased to 55% after 72 h incubation with 5.0 µM of vanicoside A), significantly stronger than vanicoside B. This stronger cytotoxic activity can be attributed to an additional acetyl group in vanicoside A. No significant differences in the cytotoxicity of vanicosides were observed against the less sensitive A375 cell line. Moreover, vanicosides caused the death of melanoma cells at concentrations from 2.5 to 50 µM, without harming the primary fibroblast line. The keratinocyte cell line (HaCaT) was more sensitive to vanicosides than fibroblasts, showing a clear decrease in viability after incubation with 25 µM of vanicoside A as well as a significant phosphatidylserine (PS) exposure, but without a measurable cell death-associated fluorescence. Vanicosides induced an apoptotic death pathway in melanoma cell lines, but because of the initial loss of cell membrane integrity, an additional cell death mechanism might be involved like permeability transition pore (PTP)-mediated necrosis that needs to be explored in the future. Molecular docking indicated that both compounds bind to the active site of the BRAFV600E kinase and MEK-1 kinase; further experiments on their specific inhibitory activity of these targets should be considered.

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