scholarly journals Proline oxidase silencing induces proline-dependent pro-survival pathways in MCF-7 cells

Oncotarget ◽  
2018 ◽  
Vol 9 (17) ◽  
pp. 13748-13757 ◽  
Author(s):  
Ilona Zareba ◽  
Katarzyna Celinska-Janowicz ◽  
Arkadiusz Surazynski ◽  
Wojciech Miltyk ◽  
Jerzy Palka
Keyword(s):  
Proline Oxidase ◽  
Survival Pathways ◽  
Mcf 7

scholarly journals Survival Pathways Are Differently Affected by Microgravity in Normal and Cancerous Breast Cells

2021 ◽  
Vol 22 (2) ◽  
pp. 862
Author(s):  
Noemi Monti ◽  
Maria Grazia Masiello ◽  
Sara Proietti ◽  
Angela Catizone ◽  
Giulia Ricci ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Cell Types ◽  
Biological Properties ◽  
Survival Strategies ◽  
Adherent Cells ◽  
Floating Structures ◽  
Survival Pathways ◽  
Breast Cells ◽  
Induced Apoptosis ◽  
Mcf 7

Metazoan living cells exposed to microgravity undergo dramatic changes in morphological and biological properties, which ultimately lead to apoptosis and phenotype reprogramming. However, apoptosis can occur at very different rates depending on the experimental model, and in some cases, cells seem to be paradoxically protected from programmed cell death during weightlessness. These controversial results can be explained by considering the notion that the behavior of adherent cells dramatically diverges in respect to that of detached cells, organized into organoids-like, floating structures. We investigated both normal (MCF10A) and cancerous (MCF-7) breast cells and found that appreciable apoptosis occurs only after 72 h in MCF-7 cells growing in organoid-like structures, in which major modifications of cytoskeleton components were observed. Indeed, preserving cell attachment to the substrate allows cells to upregulate distinct Akt- and ERK-dependent pathways in MCF-7 and MCF-10A cells, respectively. These findings show that survival strategies may differ between cell types but cannot provide sufficient protection against weightlessness-induced apoptosis alone if adhesion to the substrate is perturbed.

scholarly journals Proline oxidase silencing inhibits p53-dependent apoptosis in MCF-7 breast cancer cells

Amino Acids ◽  
2021 ◽  
Author(s):  
Ilona Oscilowska ◽  
Thi Y. L. Huynh ◽  
Weronika Baszanowska ◽  
Izabela Prokop ◽  
Arkadiusz Surazynski ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Survival Function ◽  
Underlying Mechanism ◽  
Proline Oxidase ◽  
Down Regulation ◽  
P53 Expression ◽  
Proline Concentration ◽  
Mcf 7

AbstractProline oxidase (POX) is mitochondrial proline-degrading enzyme of dual apoptosis/survival function. POX expression and proline availability are considered an underlying mechanism for differential POX functions. The mechanism for POX-dependent regulation of cell death/survival was studied in wild-type (MCF-7WT) and shRNA POX-silenced breast cancer cells (MCF-7iPOX). Proline concentration and proteomic analyses were determined by LC/MS/QTOF and LC/MS/ORBITRA, respectively. Inhibition of collagen biosynthesis (proline utilizing process) by 2-methoxyestradiol (2ME) contributed to induction of apoptosis in MCF-7WT cells, as detected by increase in the expression of active caspase-3, -9 and p53. The process was not shown in MCF-7iPOX. In MCF-7iPOX cells prolidase activity and expression as well as proline concentration were drastically increased, compared to MCF-7WT cells. Down-regulation of p53 in MCF-7iPOX cells was corroborated by proteomic analysis showing decrease in the expression of p53-related proteins. The mechanism for down-regulation of p53 expression in MCF-7iPOX cells was found at the level of p53–PEPD complex formation that was counteracted by hydrogen peroxide treatment. In this study, we found that silencing POX modulate pro-survival phenotype of MCF-7 cells and suggest that the mechanism of this process undergoes through down-regulation of p53-dependent signaling.

scholarly journals Clove Buds Affect MCF-7 Breast Cancer Cell Stress/Survival Pathways and Induce Oxidative Stress, DNA Damage, Cell Cycle Arrest, and Apoptosis

Proceedings ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 51
Author(s):  
Martin Kello ◽  
Lenka Varinska ◽  
Matus Coma ◽  
Peter Kubatka ◽  
Klaudia Petrova ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cell Cycle ◽  
Dna Damage ◽  
Cell Stress ◽  
Damage Cell ◽  
Cycle Arrest ◽  
Survival Pathways ◽  
Stress Survival ◽  
Mcf 7

Breast cancer is reportedly the second most diagnosed cancer worldwide. Several recent [...]

Study of the mechanism responsible for multidrug resistance in breast cancer MCF-7 cell mediated by 14-3-3σ

2010 ◽  
Vol 34 (8) ◽  
pp. S47-S47
Author(s):  
Guopei Zheng ◽  
Sisi Yi ◽  
Yafei Li ◽  
Fangren Kong ◽  
Yanhui Yu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Multidrug Resistance ◽  
Mcf 7

Expression und Rolle von Bridge-1 in der Mammakarzinomzelllinie MCF-7

2009 ◽  
Vol 69 (09) ◽  
Author(s):  
C Banz ◽  
B Münchow ◽  
K Diedrich
Keyword(s):  
Mcf 7

PGRMC1 ermöglicht agonistische Effekte von Tamoxifen auf MCF-7 Mammakarzinomzellen

2014 ◽  
Vol 74 (S 01) ◽  
Author(s):  
C Blassl ◽  
H Seeger ◽  
AO Mueck ◽  
T Fehm ◽  
H Neubauer
Keyword(s):  
Mcf 7

Effects of novel phenolic chalcone derivatives upon MCF-7 Cell viability

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
A Hussain ◽  
M Grootveld ◽  
R Arroo ◽  
K Beresford ◽  
K Ruparelia ◽  
...  
Keyword(s):  
Cell Viability ◽  
Chalcone Derivatives ◽  
Mcf 7
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