scholarly journals ATP-competitive Plk1 inhibitors induce caspase 3-mediated Plk1 cleavage and activation in hematopoietic cell lines

Oncotarget ◽  
2017 ◽  
Vol 9 (13) ◽  
pp. 10920-10933
Author(s):  
Maeva Dufies ◽  
Damien Ambrosetti ◽  
Sonia Boulakirba ◽  
Anne Calleja ◽  
Coline Savy ◽  
...  
Keyword(s):  
Cell Lines ◽  
Caspase 3 ◽  
Hematopoietic Cell ◽  
Hematopoietic Cell Lines

Malignant hematopoietic cell lines: In vitro models for the study of primary mediastinal B-cell lymphomas

2015 ◽  
Vol 39 (1) ◽  
pp. 18-29 ◽  
Author(s):  
Hans G. Drexler ◽  
Stefan Ehrentraut ◽  
Stefan Nagel ◽  
Sonja Eberth ◽  
Roderick A.F. MacLeod
Keyword(s):  
B Cell ◽  
Cell Lines ◽  
In Vitro Models ◽  
Hematopoietic Cell ◽  
B Cell Lymphomas ◽  
Hematopoietic Cell Lines

scholarly journals Collagen type VI in the human bone marrow microenvironment: a strong cytoadhesive component

Blood ◽  
1995 ◽  
Vol 86 (5) ◽  
pp. 1740-1748 ◽  
Author(s):  
G Klein ◽  
CA Muller ◽  
E Tillet ◽  
ML Chu ◽  
R Timpl
Keyword(s):  
Bone Marrow ◽  
Cell Lines ◽  
Triple Helix ◽  
Collagen Type ◽  
Hematopoietic Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Collagen Vi ◽  
Collagen Type Vi ◽  
Hematopoietic Cell Lines

Collagen type VI, which forms characteristic microfibrillar structures, is assembled from three individual alpha(VI) chains that form a short triple helix and two adjacent globular domains. Expression of all three alpha (VI) collagen chains in the human bone marrow (BM) microenvironment could be detected by chain-specific antibodies in tissue sections and in the adherent stromal layer of long-term BM cultures. In functional studies, collagen type VI was shown to be a strong adhesive substrate for various hematopoietic cell lines and light-density BM mononuclear cells. The adhesive site within the molecule seems to be restricted to the triple helical domain of all three alpha (VI) chains, because individual alpha (VI) chains were not active in the attachment assays. Adhesion of the hematopoietic cell lines to collagen VI was dose-dependent and could be inhibited by heparin. Although the triple helix contains several RGD sequences, adhesion of the hematopoietic cell types to collagen VI could be blocked neither by RGD-containing peptides nor by a neutralizing antibody to the beta 1 integrin subunit. In combination with an antiadhesive substrate, the binding properties of collagen VI could be downregulated. These data suggest that this collagen type may play an important role in the adhesion of hematopoietic cells within the BM microenvironment.

scholarly journals Siderocalin/Lcn2/NGAL/24p3 Does Not Drive Apoptosis Through Gentisic Acid Mediated Iron Withdrawal in Hematopoietic Cell Lines

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e43696 ◽  
Author(s):  
Colin Correnti ◽  
Vera Richardson ◽  
Allyson K. Sia ◽  
Ashok D. Bandaranayake ◽  
Mario Ruiz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hematopoietic Cell ◽  
Gentisic Acid ◽  
Hematopoietic Cell Lines

scholarly journals Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines.

1989 ◽  
Vol 86 (21) ◽  
pp. 8536-8540 ◽  
Author(s):  
W. F. Shen ◽  
C. Largman ◽  
P. Lowney ◽  
J. C. Corral ◽  
K. Detmer ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hematopoietic Cell ◽  
Hematopoietic Cell Lines

scholarly journals Induction of programmed cell death in human hematopoietic cell lines by fibronectin via its interaction with very late antigen 5.

1994 ◽  
Vol 179 (6) ◽  
pp. 1757-1766 ◽  
Author(s):  
H Sugahara ◽  
Y Kanakura ◽  
T Furitsu ◽  
K Ishihara ◽  
K Oritani ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Cell Lines ◽  
Growth Suppression ◽  
Hematopoietic Cell ◽  
Leukemia Cell Line ◽  
Gm Csf ◽  
Late Antigen ◽  
Antigen 5 ◽  
Hematopoietic Cell Lines

Extracellular matrix (ECM) molecules such as fibronectin (FN), collagens, and laminin have important roles in hematopoiesis. However, little is known about the precise mechanisms by which ECM molecules regulate proliferation of human hematopoietic progenitor cells. In this study, we have investigated the effects of ECM molecules, particularly of FN, on the proliferation of a myeloid leukemia cell line, M07E, which proliferates in response to either human granulocyte/macrophage colony-stimulating factor (GM-CSF) or stem cell factor (SCF). The [3H]thymidine incorporation and cell enumeration assays showed that FN strikingly inhibited GM-CSF- or SCF-induced proliferation of M07E cells in a dose-dependent manner, whereas little or no inhibition was induced by collagen types I and IV. The growth suppression of M07E cells was not due to the inhibitory effect of FN on ligand binding or very early events in the signal transduction pathways from the GM-CSF or SCF receptors. DNA content analysis using flow cytometry after staining with propidium iodide revealed that the treatment of M07E cells with FN did not block the entry of the cells into the cell cycle after stimulation with GM-CSF or SCF, whereas the treatment resulted in the appearance of subdiploid peak. Furthermore, FN was found to induce oligonucleosomal DNA fragmentation and chromatin condensation in the cells even in the presence of GM-CSF or SCF, suggesting the involvement of programmed cell death (apoptosis) in the FN-induced growth suppression. The growth suppression or apoptosis induced by FN was rescued by the addition of either anti-FN antibody, anti-very late antigen 5 monoclonal antibody (anti-VLA5 mAb), or GRGDSP peptide, but not by that of anti-VLA4 mAb or GRGESP peptide, suggesting that the FN effects on M07E cells were mediated through VLA5. In addition, the FN-induced apoptosis was detectable in VLA5-positive human hematopoietic cell lines other than M07E cells, but not in any of the VLA5-negative cell lines. These results suggest that FN is capable of inducing apoptosis via its interaction with VLA5, and also raise the possibility that the FN-VLA5 interaction may contribute, at least in part, to negative regulation of hematopoiesis.

Amphotropic or Gibbon Ape Leukemia Virus Retrovirus Binding and Transduction Correlates with the Level of Receptor mRNA in Human Hematopoietic Cell Lines

1997 ◽  
Vol 23 (3) ◽  
pp. 422-433 ◽  
Author(s):  
Denise E. Sabatino ◽  
Bao-Khanh Q. Do ◽  
Louise C. Pyle ◽  
Nancy E. Seidel ◽  
Laurie J. Girard ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Virus ◽  
Hematopoietic Cell ◽  
Receptor Mrna ◽  
Gibbon Ape Leukemia Virus ◽  
Hematopoietic Cell Lines
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