scholarly journals Baicalein inhibits progression of osteosarcoma cells through inactivation of the Wnt/β-catenin signaling pathway

Oncotarget ◽  
2017 ◽  
Vol 8 (49) ◽  
pp. 86098-86116 ◽  
Author(s):  
Guo Dai ◽  
Di Zheng ◽  
Qianliang Wang ◽  
Jian Yang ◽  
Gaiwei Liu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Osteosarcoma Cells

scholarly journals HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway

2016 ◽  
Vol 37 (2) ◽  
pp. 1107-1113 ◽  
Author(s):  
Dong Ning Liu ◽  
Yun Fei Zhou ◽  
Ai Fen Peng ◽  
Xin Hua Long ◽  
Xuan Yin Chen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Osteosarcoma Cells ◽  
Malignant Phenotype

scholarly journals Effect of CXCR4 on Apoptosis in Osteosarcoma Cells via the PI3K/Akt/NF-κβ Signaling Pathway

2018 ◽  
Vol 46 (6) ◽  
pp. 2250-2260 ◽  
Author(s):  
Chunming Jiang ◽  
Shenglin Ma ◽  
Runlei Hu ◽  
Xuepeng Wang ◽  
Maoqiang Li ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Luciferase Reporter ◽  
Osteosarcoma Cells ◽  
Down Regulation ◽  
Expression Levels ◽  
Apoptotic Protein ◽  
Effective Manner ◽  
Human Osteosarcoma Cells

Background/Aims: Osteosarcoma, the most common primary bone malignancy, arises from primitive transformed cells of mesenchymal origin with the worldwide increasing morbidity and mortality. Previous studies found apoptosis of osteosarcoma cells was essential for an effective manner to improve the progress of osteosarcoma, and CXCR4 has been demonstrated to be relevant with various tumor progress and metastasis. Methods: The proliferation of cells transfected with CXCR4 shRNA and control shRNA were measured by BrdU assay. Apoptosis was detected by flow cytometry. Apoptotic protein expression levels were detected by Western blot. Caspase activity was detected by Colorimetric Assay Kits using microplate reader. Activation of NF-κβ signaling after CXCR4 down-regulation in osteosarcoma cells was examined by constructing NF-κβ promoter luciferase reporter plasmid. The expression and activation of NF-κβ Signaling relevant protein were analyzed to investigate the relationship between Akt and NF-κβ signaling after the down-regulation of CXCR4 in osteosarcoma cells. Results: Down-regulation of CXCR4 significantly reduced the cell proliferation, while remarkably increased the cell apoptosis and apoptotic protein expression levels in osteosarcoma cells. Furthermore, down-regulation of CXCR4 induced cell apoptosis was caspase dependent in osteosarcoma cells. This study also showed CXCR4 down-regulation induced apoptosis through inhibiting PI3K/Akt/NF-κβ signaling pathway. In addition, endoplasmic reticulum stress (ERS) activation was involved in cell apoptosis induced down-regulation of CXCR4. Knockdown of partial ERS relevant proteins followed down-regulation of CXCR4 significantly inhibited cell apoptosis and the apoptotic protein expression levels. Conclusions: Taken together, the results demonstrated that down-regulation of CXCR4 could induce apoptosis of human osteosarcoma cells through inhibiting PI3K/Akt/NF-κβ signaling pathway, indicating that CXCR4 could be vital for the clinical therapy of osteosarcoma.

scholarly journals Simvastatin Impairs Growth Hormone-Activated Signal Transducer and Activator of Transcription (STAT) Signaling Pathway in UMR-106 Osteosarcoma Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e87769 ◽  
Author(s):  
María Claudia Sandoval-Usme ◽  
Adriana Umaña-Pérez ◽  
Borja Guerra ◽  
Orlando Hernández-Perera ◽  
José Manuel García-Castellano ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Signaling Pathway ◽  
Signal Transducer ◽  
Osteosarcoma Cells ◽  
Stat Signaling ◽  
Umr 106
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