scholarly journals Silencing PPA1 inhibits human epithelial ovarian cancer metastasis by suppressing the Wnt/β-catenin signaling pathway

Oncotarget ◽  
2017 ◽  
Vol 8 (44) ◽  
pp. 76266-76278 ◽  
Author(s):  
Haiying Niu ◽  
Wei Zhou ◽  
Yingxi Xu ◽  
Zhiqi Yin ◽  
Wenzhi Shen ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Signaling Pathway ◽  
Cancer Metastasis

scholarly journals CCL18 from tumor‐cells promotes epithelial ovarian cancer metastasis via mTOR signaling pathway

2015 ◽  
Vol 55 (11) ◽  
pp. 1688-1699 ◽  
Author(s):  
Qi Wang ◽  
Yong Tang ◽  
Hongjing Yu ◽  
Qiaoyun Yin ◽  
Mengdi Li ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Signaling Pathway ◽  
Tumor Cells ◽  
Cancer Metastasis ◽  
Mtor Signaling ◽  
Mtor Signaling Pathway

scholarly journals β-hCG promotes epithelial ovarian cancer metastasis through ERK/MMP2 signaling pathway

Cell Cycle ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 46-59 ◽  
Author(s):  
Weimin Wu ◽  
Hao Gao ◽  
Xiaofeng Li ◽  
Shumin Peng ◽  
Jing Yu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Signaling Pathway ◽  
Cancer Metastasis ◽  
Β Hcg

scholarly journals AMPK-independent LKB1 activity is required for efficient epithelial ovarian cancer metastasis

2019 ◽  
Author(s):  
Adrian Buensuceso ◽  
Yudith Ramos Valdes ◽  
Gabriel E. DiMattia ◽  
Trevor G. Shepherd
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cell Survival ◽  
Cell Lines ◽  
Cancer Metastasis ◽  
Metastatic Potential ◽  
Metastatic Tumour ◽  
Ampk Signaling ◽  
Dual Specificity ◽  
Liver Kinase B1

ABSTRACTEpithelial ovarian cancer (EOC) spreads by direct dissemination of malignant cells and multicellular clusters, known as spheroids, into the peritoneum followed by implantation and growth on abdominal surfaces. Using a spheroid model system of EOC metastasis, we discovered that Liver kinase B1 (LKB1), encoded by theSTK11gene, and its canonical substrate AMP-activated protein kinase (AMPK) are activated in EOC spheroids, yet only LKB1 is required for cell survival. We have now generatedSTK11-knockout cell lines using normal human FT190 cells and three EOC cell lines, OVCAR8, HeyA8, and iOvCa147.STK11KO did not affect growth and viability in adherent culture, but it decreased anchorage-independent growth of EOC cells. EOC spheroids lacking LKB1 had markedly impaired growth and viability, whereas there was no difference in normal FT190 spheroids. To test whether LKB1 loss affects EOC metastasis, we performed intraperitoneal injections of OVCAR8-, HeyA8-, and iOvCa147-STK11KO cells, and respective controls. LKB1 loss exhibited a dramatic reduction on tumour burden and metastatic potential; in particular, OVCAR8-STK11KO tumours had evidence of extensive necrosis, apoptosis and hypoxia. Interestingly, LKB1 loss did not affect AMPKα phosphorylation in EOC spheroids and tumour xenografts, indicating that LKB1 signaling to support EOC cell survival in spheroids and metastatic tumour growth occurs via other downstream mediators. We identified the dual-specificity phosphatase DUSP4 as a commonly upregulated protein due to LKB1 loss; indeed,DUSP4knockdown in HeyA8-STK11KO cells restored spheroid formation and viability. Our results strongly indicate that intact LKB1 activity independent of downstream AMPK signaling is required during EOC metastasis.

scholarly journals AMPK-Independent LKB1 Activity Is Required for Efficient Epithelial Ovarian Cancer Metastasis

2019 ◽  
Vol 18 (3) ◽  
pp. 488-500 ◽  
Author(s):  
Adrian Buensuceso ◽  
Yudith Ramos-Valdes ◽  
Gabriel E. DiMattia ◽  
Trevor G. Shepherd
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cancer Metastasis
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