scholarly journals In silico gene expression analysis reveals glycolysis and acetate anaplerosis in IDH1 wild-type glioma and lactate and glutamate anaplerosis in IDH1-mutated glioma

Oncotarget ◽  
2017 ◽  
Vol 8 (30) ◽  
pp. 49165-49177 ◽  
Author(s):  
Mohammed Khurshed ◽  
Remco J. Molenaar ◽  
Krissie Lenting ◽  
William P. Leenders ◽  
Cornelis J.F. van Noorden
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
In Silico ◽  
Gene Expression Analysis ◽  
Wild Type

Molecular cloning, gene expression analysis, and in silico characterization of UDP‐N‐acetylglucosamine pyrophosphorylase from Bombyx mori

2019 ◽  
Vol 66 (5) ◽  
pp. 880-899
Author(s):  
Bhagath Kumar Palaka ◽  
Anbumani Velmurugan Ilavarasi ◽  
Tuleshwori Devi Sapam ◽  
Kasi Viswanath Kotapati ◽  
Venkata Satyanarayana Nallala ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Molecular Cloning ◽  
Expression Analysis ◽  
In Silico ◽  
Gene Expression Analysis ◽  
In Silico Characterization

scholarly journals Some aspects of metal ion transport and in silico gene expression analysis of potassium/sodium ion transporters, channels and exchangers in root nodules

2021 ◽  
Vol 66 (1) ◽  
Author(s):  
Natalia Trifonova ◽  
Maria Koroleva ◽  
Elena Fedorova
Keyword(s):  
Gene Expression ◽  
Ion Transport ◽  
Expression Analysis ◽  
In Silico ◽  
Metal Ion ◽  
Gene Expression Analysis ◽  
Root Nodule ◽  
Root Nodules ◽  
Sodium Ion ◽  
Metal Ion Transport

Rhizobia establish a symbiotic relationship with legumes, which results in the formation of root nodules, the ecological niche for intracellular rhizobia. The infected cell of a root nodule is a special integral unit of plant and nitrogen fixing rhizobia. Nodules tend to be very sensitive to ionic stresses, such as salt stress. High vulnerability toward ionic stresses might be due to defects in ion balance and transport in the infected tissue. The purpose of this minireview is to summarize the current data regarding metal ion transport in the root nodule, with particular emphasis on potassium/sodium ion transport. A bioinformatic approach and in silico gene expression analysis have been used to obtain some insight for K+/Na+ transportеr channels and exchangers in root nodule developmental zones.

scholarly journals IN SILICO DESIGN, SYNTHESIS AND EVALUATION OF IN VITRO GLUCOSE UPTAKE, GENE EXPRESSION, AND α-GLUCOSIDASE INHIBITORY ACTIVITY OF NOVEL 2-AMINOBENZIMIDAZOLE DERIVATIVES

2019 ◽  
pp. 107-114
Author(s):  
SREEJA S ◽  
ANTON SMITH A ◽  
MATHAN S
Keyword(s):  
Gene Expression ◽  
Glucose Uptake ◽  
Expression Analysis ◽  
Inhibitory Activity ◽  
In Silico ◽  
Gene Expression Analysis ◽  
Lethal Dose ◽  
Standard Drug ◽  
Lipinski’S Rule

Objective: The present study was aimed to design and evaluate the antidiabetic potential of novel 2-aminobenzimidazole derivatives by in silico method. Materials and Methods: Various in silico tools such as Chemsketch, Molinspiration, Prediction of activity spectra for substances, OpenBabel, Discovery Studio was used in the designing and evaluation of the biological activity. The retrieved hits were further filtered by absorption, distribution, metabolism, and excretion descriptors. The designed molecules having required physicochemical properties, drug-likeness, and obeying Lipinski’s rule of five were selected for the synthesis. The synthesized compounds were subjected to determination of yield, melting point and characterized by infrared, 1HNMR, 13CNMR, and mass spectroscopic methods. The selected derivatives were subjected to in vitro glucose uptake, 50% lethal dose (LD50) determination, gene expression analysis, and α-glucosidase inhibitory assay. Results: Totally, 32 novel analogs of 2-aminobenzimidazole were designed and 17 compounds were selected for docking analysis; and finally, five derivatives (3a, 3c, 3e, 3f, and 3h) were selected for synthesis. Among them, the compounds 3a and 3f were selected for in vitro glucose uptake analysis. Finally, the compound 3f was selected for LD50 determination, gene expression analysis, and α-glucosidase inhibitory assay. The selected derivative 3f showed a significant α-glucosidase inhibitory activity compared with the standard drug acarbose. Conclusion: These results are useful for further investigation in the future, and hopefully, these studies could discover a new specific leads in antidiabetic category as α-glucosidase inhibitor.

The presence and distribution of G protein-coupled receptor 35 (GPR35) in the human cornea – Evidences from in silico gene expression analysis and immunodetection

2019 ◽  
Vol 179 ◽  
pp. 188-192
Author(s):  
Anna Matysik-Woźniak ◽  
Artur Wnorowski ◽  
Waldemar A. Turski ◽  
Krzysztof Jóźwiak ◽  
Anselm Jünemann ◽  
...  
Keyword(s):  
Gene Expression ◽  
G Protein ◽  
Expression Analysis ◽  
In Silico ◽  
Gene Expression Analysis ◽  
Human Cornea ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

In silico gene expression analysis in Codonopsis lanceolata root

2010 ◽  
Vol 38 (5) ◽  
pp. 3541-3549 ◽  
Author(s):  
Subramaniyam Sathiyamoorthy ◽  
Jun-Gyo In ◽  
Ok Ran Lee ◽  
Bum-Soo Lee ◽  
Sri Renuka Devi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
In Silico ◽  
Gene Expression Analysis ◽  
Codonopsis Lanceolata
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