scholarly journals Anti-oxidative stress response genes: bioinformatic analysis of their expression and relevance in multiple cancers

Oncotarget ◽  
2013 ◽  
Vol 4 (12) ◽  
pp. 2577-2590 ◽  
Author(s):  
Barak Rotblat ◽  
Thomas G. P. Grunewald ◽  
Gabriel Leprivier ◽  
Gerry Melino ◽  
Richard A. Knight
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Bioinformatic Analysis ◽  
Oxidative Stress Response ◽  
Stress Response Genes ◽  
Multiple Cancers

Oxidative Stress Response Genes Upregulated During Unloading, Immobilization, and Spinal Cord Injury

2008 ◽  
Vol 40 (Supplement) ◽  
pp. S476 ◽  
Author(s):  
Kimberly A. Sewright ◽  
Maria L. Urso ◽  
Paul D. Thompson ◽  
Cherie Bilbe ◽  
Yi-Wen Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Stress Response Genes ◽  
Cord Injury

The expression of oxidative stress response genes is modulated by a combination of resveratrol and N-acetylcysteine to ameliorate ototoxicity in the rat cochlea

2018 ◽  
Vol 358 ◽  
pp. 10-21 ◽  
Author(s):  
Fernando García-Alcántara ◽  
Silvia Murillo-Cuesta ◽  
Sara Pulido ◽  
Jose M. Bermúdez-Muñoz ◽  
Raquel Martínez-Vega ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Stress Response Genes ◽  
Rat Cochlea

scholarly journals Endoribonuclease YbeY Is Essential for RNA Processing and Virulence in Pseudomonas aeruginosa

mBio ◽  
2020 ◽  
Vol 11 (3) ◽  
Author(s):  
Yushan Xia ◽  
Yuding Weng ◽  
Congjuan Xu ◽  
Dan Wang ◽  
Xiaolei Pan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pseudomonas Aeruginosa ◽  
Stress Response ◽  
Small Rnas ◽  
Posttranscriptional Regulation ◽  
Oxidative Stress Response ◽  
Bacterial Virulence ◽  
Rrna Processing ◽  
Content Type ◽  
Stress Response Genes

ABSTRACT Posttranscriptional regulation plays an essential role in the quick adaptation of pathogenic bacteria to host environments, and RNases play key roles in this process by modifying small RNAs and mRNAs. We find that the Pseudomonas aeruginosa endonuclease YbeY is required for rRNA processing and the bacterial virulence in a murine acute pneumonia model. Transcriptomic analyses reveal that knocking out the ybeY gene results in downregulation of oxidative stress response genes, including the catalase genes katA and katB. Consistently, the ybeY mutant is more susceptible to H2O2 and neutrophil-mediated killing. Overexpression of katA restores the bacterial tolerance to H2O2 and neutrophil killing as well as virulence. We further find that the downregulation of the oxidative stress response genes is due to defective expression of the stationary-phase sigma factor RpoS. We demonstrate an autoregulatory mechanism of RpoS and find that ybeY mutation increases the level of a small RNA, ReaL, which directly represses the translation of rpoS through the 5′ UTR of its mRNA and subsequently reduces the expression of the oxidative stress response genes. In vitro assays demonstrate direct degradation of ReaL by YbeY. Deletion of reaL or overexpression of rpoS in the ybeY mutant restores the bacterial tolerance to oxidative stress and the virulence. We also demonstrate that YbeZ binds to YbeY and is involved in the 16S rRNA processing and regulation of reaL and rpoS as well as the bacterial virulence. Overall, our results reveal pleiotropic roles of YbeY and the YbeY-mediated regulation of rpoS through ReaL. IMPORTANCE The increasing bacterial antibiotic resistance imposes a severe threat to human health. For the development of effective treatment and prevention strategies, it is critical to understand the mechanisms employed by bacteria to grow in the human body. Posttranscriptional regulation plays an important role in bacterial adaptation to environmental changes. RNases and small RNAs are key players in this regulation. In this study, we demonstrate critical roles of the RNase YbeY in the virulence of the pathogenic bacterium Pseudomonas aeruginosa. We further identify the small RNA ReaL as the direct target of YbeY and elucidate the YbeY-regulated pathway on the expression of bacterial virulence factors. Our results shed light on the complex regulatory network of P. aeruginosa and indicate that inference with the YbeY-mediated regulatory pathway might be a valid strategy for the development of a novel treatment strategy.

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