scholarly journals Prognostic role of programmed-death ligand 1 (PD-L1) expressing tumor infiltrating lymphocytes in testicular germ cell tumors

Oncotarget ◽  
2017 ◽  
Vol 8 (13) ◽  
pp. 21794-21805 ◽  
Author(s):  
Michal Chovanec ◽  
Zuzana Cierna ◽  
Viera Miskovska ◽  
Katarina Machalekova ◽  
Daniela Svetlovska ◽  
...  
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Tumor Infiltrating Lymphocytes ◽  
Testicular Germ Cell Tumors ◽  
Prognostic Role ◽  
Testicular Germ Cell ◽  
Programmed Death Ligand 1 ◽  
Programmed Death ◽  
Infiltrating Lymphocytes

Prognostic role of PD-L1 expressing tumor infiltrating lymphocytes in testicular germ cell tumors.

2016 ◽  
Vol 34 (15_suppl) ◽  
pp. 4544-4544 ◽  
Author(s):  
Michal Chovanec ◽  
Zuzana Cierna ◽  
Vera Miskovska ◽  
Katarina Machalekova ◽  
Katarina Kalavska ◽  
...  
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Tumor Infiltrating Lymphocytes ◽  
Testicular Germ Cell Tumors ◽  
Prognostic Role ◽  
Testicular Germ Cell ◽  
Infiltrating Lymphocytes

Differences in PD-L1–Expressing Macrophages and Immune Microenvironment in Testicular Germ Cell Tumors

2019 ◽  
Vol 153 (3) ◽  
pp. 387-395 ◽  
Author(s):  
Sam Sadigh ◽  
Sahar J Farahani ◽  
Abhishek Shah ◽  
David Vaughn ◽  
Priti Lal
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Tumor Infiltrating Lymphocytes ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Immunohistochemical Markers ◽  
Targeted Treatments ◽  
Choriocarcinoma Cells ◽  
Infiltrating Lymphocytes ◽  
Nonseminomatous Germ Cell Tumors

Abstract Objectives To characterize the tumor microenvironment of testicular germ cell tumors (GCTs) using immunohistochemical markers. Methods Seventy-seven orchiectomies, including 36 nonmetastatic (NM) seminomas, 15 metastatic (M) seminomas, 13 nonmetastatic nonseminomatous germ cell tumors (NSGCTs), and 13 metastatic NSGCTs, were studied with PD-1, PD-L1, FOXP3, CD68, CD163, and mismatch repair (MMR) immunohistochemistry. FOXP3+ and PD-1+ tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) expressing CD68 and CD163 were enumerated. PDL-1 expression was evaluated on tumor cells and macrophages. Results GCTs primarily express PD-L1 on TAMs, except choriocarcinoma, where true tumor cell positivity was noted. Seminomas reveal increased intratumoral PD-L1+ TAMs compared with NSGCTs (P < .05). Activated TILs are increased in NM-seminomas compared with M-seminomas (P < .05). All GCTs retained MMR expression. Conclusions Robust PD-L1+ TAMs are significantly expanded in seminomas compared with NSGCTs. Among all GCTs, only choriocarcinoma cells reveal true positivity for PD-L1. These findings expand the realm of potentially targeted treatments for GCTs.

Systemic immune-inflammation index is prognostic in testicular germ cell tumors with PD-L1 expressing tumor infiltrating lymphocytes.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e16042-e16042 ◽  
Author(s):  
Michal Chovanec ◽  
Zuzana Cierna ◽  
Vera Miskovska ◽  
Katarina Machalekova ◽  
Katarina Kalavska ◽  
...  
Keyword(s):  
Germ Cell ◽  
Prognostic Value ◽  
Germ Cell Tumors ◽  
Tumor Infiltrating Lymphocytes ◽  
Collaborative Group ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Immune Inflammation ◽  
Validation Set ◽  
Infiltrating Lymphocytes

e16042 Background: Systemic immune-inflammation index (SII) and programmed death-ligand 1 (PD-L1) are prognostic in various types of malignancies. Recently we have shown a prognostic value of PD-L1 expression on tumor cells and tumor infiltrating lymphocytes (TILs) in testicular germ cell tumors (GCT). This study aimed to evaluate prognostic role of SII in a GCT population of patients expressing PD-L1 on TILs. Methods: SII was calculated using platelet (P), neutrophil (N) and lymphocyte (L) counts measured prior to chemotherapy (SII = P x N/L). SII was calculated in our discovery set of 216 patients with GCT treated at National Cancer Institute and St. Elisabeths' Cancer Institute between 1999 and 2015. A model with median obtained from the discovery data was tested in an independent validation set of 181 patients that were included in a retrospective study evaluating PD-L1 on TILs in GCT. PD-L1 on TILs was detected by immunohistochemistry and scored semiquantitatively by weighted histoscore method. SII was dichotomized into low and high categories based on median value. Results: Low SII ( < 1003) was found in 133 patients (73.5%) as opposed to 48 patients (26.5%) with high SII (≥ 1003). Ten (5.5%) and 171 patients (94.5%) from the validaton set had low (HS < 150) and high (HS ≥ 160) expression of PD-L1 on TILs, respectively. Discovery group of patients with high SII had significantly shorter PFS (HR = 4.48, 95% CI 2.44 – 8.23, p = 0.0000) and OS (HR = 6.10 95% CI 3.11 – 11.95, p = 0.0000) opposite to patients with low SII. PFS from validation set confirmed shorter PFS (HR = 3.03, 95% CI 3.86 – 7.46, p = 0.0062) and OS (HR = 6.49 95% CI 2.10 – 20.03, p = 0.0001) in patients with high versus low SII. A combined prognostic value of PD-L1 TILs and SII uncovered three prognostic groups. The best prognosis was observed in patients with low SII and high PD-L1 on TILs, the worst prognosis was seen in patients with high SII and low PD-L1 on TILs. Patients with SII and PD-L1 on TILs both values high or low had intermediate prognosis. Conclusions: SII was prognostic in our patients with GCT independently of international germ cell cancer collaborative group criteria, suggesting involvement of immune mechanisms in the behavior of GCT.

scholarly journals The Role of the Mouse Y Chromosome on Susceptibility to Testicular Germ Cell Tumors

2009 ◽  
Vol 69 (8) ◽  
pp. 3614-3618 ◽  
Author(s):  
Philip D. Anderson ◽  
Man-Yee Lam ◽  
Christophe Poirier ◽  
Colin E. Bishop ◽  
Joseph H. Nadeau
Keyword(s):  
Germ Cell ◽  
Y Chromosome ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell

scholarly journals Role of microRNAs in mammalian spermatogenesis and testicular germ cell tumors

Reproduction ◽  
2015 ◽  
Vol 149 (3) ◽  
pp. R127-R137 ◽  
Author(s):  
Li Wang ◽  
Chen Xu
Keyword(s):  
Germ Cell ◽  
Expression Profiles ◽  
Regulation Of Gene Expression ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Mirna Regulation ◽  
Testicular Germ Cell ◽  
Complex Process ◽  
Regulatory Functions

microRNAs (miRNAs) are a class of small endogenous RNAs, 19–25 nucleotides in size, which play a role in the regulation of gene expression at transcriptional and post-transcriptional levels. Spermatogenesis is a complex process through which spermatogonial stem cells (SSCs) proliferate and differentiate into mature spermatozoa. A large number of miRNAs are abundantly expressed in spermatogenic cells. Growing evidence supports the essential role of miRNA regulation in normal spermatogenesis and male fertility and cumulative research has shown that this form of regulation contributes to the etiology of testicular germ cell tumors (TGCTs). In this review, we addressed recent advancements of miRNA expression profiles in testis and focused on the regulatory functions of miRNA in the process of SSC renewal, spermatogonial mitosis, spermatocyte meiosis, spermiogenesis, and the occurrence of TGCTs.

The Role of Alkaline Phosphatase Isoenzymes as Tumor Markers for Testicular Germ Cell Tumors

1991 ◽  
Vol 146 (1) ◽  
pp. 57-60 ◽  
Author(s):  
K. Koshida ◽  
A. Nishino ◽  
H. Yamamoto ◽  
T. Uchibayashi ◽  
K. Naito ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Germ Cell ◽  
Tumor Markers ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Alkaline Phosphatase Isoenzymes

scholarly journals The role of microenvironment in testicular germ cell tumors

2010 ◽  
Vol 10 (6) ◽  
pp. 529-536 ◽  
Author(s):  
Alejandro Díez-Torre ◽  
Unai Silván ◽  
María Díaz-Núñez ◽  
Juan Arechaga
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell
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