scholarly journals A methodological study of genome-wide DNA methylation analyses using matched archival formalin-fixed paraffin embedded and fresh frozen breast tumors

Oncotarget ◽  
2017 ◽  
Vol 8 (9) ◽  
pp. 14821-14829 ◽  
Author(s):  
Allyson C. Espinal ◽  
Dan Wang ◽  
Li Yan ◽  
Song Liu ◽  
Li Tang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Breast Tumors ◽  
Methodological Study ◽  
Genome Wide ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Fresh Frozen ◽  
Formalin Fixed

Genome-wide DNA methylation analysis of formalin-fixed paraffin embedded colorectal cancer tissue

2014 ◽  
Vol 53 (7) ◽  
pp. 537-548 ◽  
Author(s):  
Troy D. Dumenil ◽  
Leesa F. Wockner ◽  
Mark Bettington ◽  
Diane M. McKeone ◽  
Kerenaftali Klein ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Cancer Tissue ◽  
Methylation Analysis ◽  
Colorectal Cancer Tissue ◽  
Genome Wide ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Dna Methylation Analysis ◽  
Formalin Fixed

scholarly journals Assessment of concordance between fresh-frozen and formalin-fixed paraffin embedded tumor DNA methylation using a targeted sequencing approach

Oncotarget ◽  
2017 ◽  
Vol 8 (29) ◽  
pp. 48126-48137 ◽  
Author(s):  
Bruce Moran ◽  
Sudipto Das ◽  
Dominiek Smeets ◽  
Gillian Peutman ◽  
Rut Klinger ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Targeted Sequencing ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Fresh Frozen ◽  
Tumor Dna ◽  
Formalin Fixed

Genome-wide DNA methylation analysis of archival formalin-fixed paraffin-embedded tissue using the Illumina Infinium HumanMethylation27 BeadChip

Methods ◽  
2010 ◽  
Vol 52 (3) ◽  
pp. 248-254 ◽  
Author(s):  
Christina Thirlwell ◽  
Marianne Eymard ◽  
Andrew Feber ◽  
Andrew Teschendorff ◽  
Kerra Pearce ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Analysis ◽  
Genome Wide ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Dna Methylation Analysis ◽  
Formalin Fixed

scholarly journals Clinicopathological impacts of DNA methylation alterations on pancreatic ductal adenocarcinoma: prediction of early recurrence based on genome-wide DNA methylation profiling

2021 ◽  
Vol 147 (5) ◽  
pp. 1341-1354
Author(s):  
Yutaka Endo ◽  
Mao Fujimoto ◽  
Nanako Ito ◽  
Yoriko Takahashi ◽  
Minoru Kitago ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Early Recurrence ◽  
Pancreatic Tissue ◽  
Ductal Adenocarcinoma ◽  
Cpg Sites ◽  
Genome Wide ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

AbstractPurposeThe present study was conducted to clarify the clinicopathological impacts of DNA methylation alterations on pancreatic ductal adenocarcinoma (PDAC).MethodsGenome-wide DNA methylation screening was performed using the Infinium HumanMethylation450 BeadChip, and DNA methylation quantification was verified using pyrosequencing. We analyzed fresh-frozen tissues from an initial cohort (17 samples of normal control pancreatic tissue [C] from 17 patients without PDAC, and 34 samples of non-cancerous pancreatic tissue [N] and 82 samples of cancerous tissue [T] both obtained from 82 PDAC patients) and formalin-fixed paraffin-embedded T samples from 34 patients in a validation cohort.ResultsThe DNA methylation profiles of N samples tended to differ from those of C samples, and 91,907 probes showed significant differences in DNA methylation levels between C and T samples. Epigenetic clustering of T samples was significantly correlated with a larger tumor diameter and early recurrence (ER), defined as relapse within 6 months after surgery. Three marker CpG sites, applicable to formalin-fixed paraffin-embedded surgically resected materials regardless of their tumor cell content, were identified for prediction of ER. The sensitivity and specificity for detection of patients belonging to the ER group using a panel combining these three marker CpG sites, including a CpG site in theCDK14gene, were 81.8% and 71.7% and 88.9% and 70.4% in the initial and validation cohorts, respectively.ConclusionThese findings indicate that DNA methylation alterations may have a clinicopathological impact on PDAC. Application of our criteria will ultimately allow prediction of ER after surgery to improve the outcome of PDAC patients.

scholarly journals Monoclonal and Polyclonal Antibodies for Immunohistochemical Detection of Bovine Parainfluenza Type 3 Virus in Frozen and Formalin-Fixed Paraffin-Embedded Tissues

1992 ◽  
Vol 4 (4) ◽  
pp. 393-399 ◽  
Author(s):  
Deborah M. Haines ◽  
Jane C. Kendall ◽  
Brad W. Remenda ◽  
Michelle M. Breker-Klassen ◽  
Edward G. Clark
Keyword(s):  
Polyclonal Antibodies ◽  
Accurate Identification ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Fresh Frozen ◽  
Type 3 ◽  
Murine Monoclonal Antibodies ◽  
Formalin Fixed ◽  
Immunohistochemical Testing

Accurate identification of bovine Parainfluenza type 3 virus in bovine respiratory disease requires dependable, sensitive, and specific techniques for detection in affected animals. Immunohistochemical testing can be a rapid and reliable means of demonstration of virus in tissues from suspect cases; however, this procedure is dependent upon the quality of the antisera directed against the viral antigens. The production of rabbit polyclonal and murine monoclonal antibodies directed against bovine Parainfluenza type 3 virus and techniques for their use in fresh-frozen and formalin-fixed paraffin-embedded tissues in immunofluorescence and immunoperoxidase-based immunohistochemical tests are described.

Sign in / Sign up

Export Citation Format

Share Document