scholarly journals Long non-coding RNA ZFAS1 interacts with miR-150-5p to regulate Sp1 expression and ovarian cancer cell malignancy

Oncotarget ◽  
2017 ◽  
Vol 8 (12) ◽  
pp. 19534-19546 ◽  
Author(s):  
Bairong Xia ◽  
Yan Hou ◽  
Hong Chen ◽  
Shanshan Yang ◽  
Tianbo Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Non Coding Rna ◽  
Long Non Coding Rna ◽  
Cell Malignancy

scholarly journals Vitamin D Suppresses Ovarian Cancer Growth and Invasion by Targeting Long Non-Coding RNA CCAT2

2020 ◽  
Vol 21 (7) ◽  
pp. 2334 ◽  
Author(s):  
Liye Wang ◽  
Shuang Zhou ◽  
Bin Guo
Keyword(s):  
Ovarian Cancer ◽  
Vitamin D ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Gynecologic Cancer ◽  
Poor Response ◽  
Migration And Invasion ◽  
Effective Prevention ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Ovarian cancer is the most deadly gynecologic cancer among women worldwide. Poor response to current treatment makes it necessary to discover new diagnostic biomarkers to detect the cancer early and develop new and effective prevention strategies. Calcitriol, the active metabolite of vitamin D, protects against multiple cancers through unelucidated mechanisms. The oncogenic long non-coding RNA (lncRNA) CCAT2 (colon cancer associated transcript 2) is overexpressed in ovarian cancer. Here, we foundd that calcitriol inhibited CCAT2 expression in ovarian cancer cell lines. Treatment with calcitriol inhibited ovarian cancer cell proliferation, migration, and invasion. As a result of CCAT2 inhibition, calcitriol decreased the binding of transcription factor TCF7L2 (TCF4) to the MYC promoter, resulting in the repression of c-Myc protein expression. Our results suggest a novel anti-cancer mechanism of vitamin D by targeting CCAT2 in ovarian cancer. The findings may help develop vitamin D as a practical and inexpensive nutraceutical for ovarian cancer prevention.

scholarly journals PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

Cancers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2233
Author(s):  
Narshone Soda ◽  
Muhammad Umer ◽  
Navid Kashaninejad ◽  
Surasak Kasetsirikul ◽  
Richard Kline ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Dynamic Range ◽  
Cancer Cell Lines ◽  
Plasma Samples ◽  
Isolation And Purification ◽  
Non Coding Rna ◽  
Broad Dynamic Range ◽  
Long Non Coding Rna

Long non-coding RNA HOX transcript antisense intergenic RNA (HOTAIR) is one of the promising biomarkers that has widely been used in determining the stages of many cancers, including ovarian cancer. In cancer diagnostics, the two key analytical challenges for detecting long non-coding RNA biomarkers are i) the low concentration levels (nM to fM range) in which they are found and ii) the analytical method where broad dynamic range is required (four to six orders of magnitude) due to the large variation in expression levels for different HOTAIR RNAs. To meet these challenges, we report on a biosensing platform for the visual (colorimetric) estimation and subsequent electrochemical quantification of ovarian-cancer-specific HOTAIR using a screen-printed gold electrode (SPE-Au). Our assay utilizes a two-step strategy that involves (i) magnetic isolation and purification of target HOTAIR sequences and (ii) subsequent detection of isolated sequences using a sandwich hybridization coupled with horseradish peroxidase (HRP)-catalyzed reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. The assay achieved a detection limit of 1.0 fM HOTAIR in spiked buffer samples with excellent reproducibility (% RSD ≤ 5%, for n = 3). It was successfully applied to detect HOTAIR in cancer cell lines and a panel of plasma samples derived from patients with ovarian cancer. The analytical performance of the method was validated with standard RT-qPCR. We believe that the proof of concept assay reported here may find potential use in routine clinical settings for the screening of cancer-related lncRNAs.

scholarly journals LncRNA LINC00665 Promotes Ovarian Cancer Cell Proliferation and Inhibits Apoptosis via Targeting MiR-181a-5p/FHDC

2021 ◽  
Author(s):  
Suli Wang ◽  
Yingchun Wang ◽  
Jin Lu ◽  
Jinhua Wang
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Inhibitory Effect ◽  
Cell Counting ◽  
Ovarian Cancer Cell Lines ◽  
Non Coding Rna ◽  
Vital Effect ◽  
Mirna Sponges ◽  
And Migration

Abstract Objective: Previous reports indicate that long intergenic non-coding RNA LINC00665 naturally occurred vital effect in various cancers. Herein, the role of LINC00665 in ovarian cancer progress was explored.Methods: We found that LINC00665 was upregulated in ovarian cancer cell lines. Besides that, A series of assays including flow cytometry, wound-healing, Transwell, Cell Counting Kit-8 (CCK-8), and EdU assay confirmed that the knockdown of LINC00665 could reduce the viability, proliferation and migration of SKOV-3 and OVCAR-3 cells. Accumulating evidence indicates that many lncRNAs can function as endogenous miRNA sponges by competitively binding common miRNAs. In this study, the bioinformatics analysis suggested that LNC00665 specifically binds to miR-181a-5p.Results: LINC00665 downregulated the miR-181a-5p in SKOV-3 and OVCAR-3 cells. The knockdown of miR-181a-5p evidently reverses the inhibitory effect of sh-LINC00662. Besides, FH2 Domain Containing 1 (FHDC1) has been proved to deed as an effective target of miR-181a-5p.Conclusion: Our results reveal the knockdown of LINC00665 facilitates ovarian cancer via development by sponging miR-181a-5p and upregulating FHDC1 expression; these may contribute to ovarian cancer therapy.

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