scholarly journals In Vivo magnetic resonance imaging of xenografted tumors using FTH1 reporter gene expression controlled by a tet-on switch

Oncotarget ◽  
2016 ◽  
Vol 7 (48) ◽  
pp. 78591-78604 ◽  
Author(s):  
Xiaoya He ◽  
Jinhua Cai ◽  
Hao Li ◽  
Bo Liu ◽  
Yong Qin ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Gene Expression ◽  
Magnetic Resonance ◽  
Reporter Gene ◽  
Reporter Gene Expression ◽  
Resonance Imaging

scholarly journals Molecular Imaging with Genetically Programmed Nanoparticles

2021 ◽  
Author(s):  
Donna E. Goldhawk
Keyword(s):  
Gene Expression ◽  
Molecular Imaging ◽  
Magnetic Resonance ◽  
Reporter Gene ◽  
Protein Interactions ◽  
Mammalian Cells ◽  
Reporter Gene Expression ◽  
Cell Therapies ◽  
Membrane Compartment

Nanoparticle research has greatly benefitted medical imaging platforms by generating new signals, enhancing detection sensitivity, and expanding both clinical and preclinical applications. For magnetic resonance imaging, the fabrication of superparamagnetic iron oxide nanoparticles has provided a means of detecting cells and has paved the way for magnetic particle imaging. As the field of molecular imaging grows and enables the tracking of cells and their molecular activities so does the possibility of tracking genetically programmed biomarkers. This chapter discusses the advantages and challenges of gene-based contrast, using the bacterial magnetosome model to highlight the requirements of in vivo iron biomineralization and reporter gene expression for magnetic resonance signal detection. New information about magnetosome protein interactions in non-magnetic mammalian cells is considered in the light of design and application(s) of a rudimentary magnetosome-like nanoparticle for molecular imaging. Central to this is the hypothesis that a magnetosome root structure is defined by essential magnetosome genes, whose expression positions the biomineral in a given membrane compartment, in any cell type. The use of synthetic biology for programming multi-component structures not only broadens the scope of reporter gene expression for molecular MRI but also facilitates the tracking of cell therapies.

In vivo visualization of gene expression using magnetic resonance imaging

10.1038/73780 ◽  
2000 ◽  
Vol 18 (3) ◽  
pp. 321-325 ◽  
Author(s):  
Angelique Y. Louie ◽  
Martina M. Hüber ◽  
Eric T. Ahrens ◽  
Ute Rothbächer ◽  
Rex Moats ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Gene Expression ◽  
Magnetic Resonance ◽  
Resonance Imaging

scholarly journals Visualization of microRNA-21 Dynamics in Neuroblastoma Cells Using Magnetic Resonance Imaging Based on a microRNA-21-responsive Reporter Gene

2021 ◽  
Author(s):  
Guangcheng Bao ◽  
Jun Sun ◽  
Jie Huang ◽  
Helin Zheng ◽  
Jie Wei ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Reporter Gene ◽  
Dependent Manner ◽  
Resonance Imaging ◽  
Reporter System ◽  
Intracellular Iron ◽  
Responsive Gene

Abstract Background: MicroRNAs (miRs) have been shown to be closely associated with the occurrence and development of tumors and to have potential as diagnostic and therapeutic targets. The detection and quantification of miRs by noninvasive imaging technology is crucial for deeply understanding their biological functions. Our aim was to develop a novel miR-21-responsive gene reporter system for magnetic resonance imaging (MRI) visualization of the miR-21 dynamics in neuroblastoma.Methods: The reporter gene ferritin heavy chain (FTH1) was modified by the addition of 3 copies of the sequence completely complementary to miR-21 (3xC_miR-21) to its 3'-untranslated region (3' UTR) and transduced into SK-N-SH cells to obtain SK-N-SH/FTH1-3xC_miR-21 cells. Then, the antagomiR-21 was delivered into cells by graphene oxide functionalized with polyethylene glycol and dendrimer. Before and after antagomiR-21 delivery, FTH1 expression, MRI contrast and intracellular iron uptake were assayed in vitro and in vivo.Results: In the SK-N-SH/FTH1-3xC_miR-21 cells, FTH1 expression was in an “off” state due to the combination of intratumoral miR-21 with the 3' UTR of the reporter gene. AntagomiR-21 delivered into the cells bound to miR-21 and thereby released it from the 3' UTR of the reporter gene, thus “switching on” FTH1 expression in a dose-dependent manner. This phenomenon resulted in intracellular iron accumulation and allowed MRI detection in vitro and in vivo.Conclusions: MRI based on the miR-21-responsive gene reporter may be a potential method for visualization of the endogenous miR-21 dynamics.

Monitoring stem cell migration in the nervous system by in vivo magnetic resonance imaging

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S692-S692
Author(s):  
Mathias Hoehn ◽  
Uwe Himmelreich ◽  
Ralph Weber ◽  
Pedro Ramos-Cabrer ◽  
Susanne Wegener ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Nervous System ◽  
Stem Cell ◽  
Cell Migration ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Stem Cell Migration
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