scholarly journals Post-transcriptional mapping reveals critical regulators of metastasis

Oncoscience ◽  
2015 ◽  
Vol 2 (10) ◽  
pp. 831-832 ◽  
Author(s):  
George S. Hussey ◽  
Breege V. Howley ◽  
Philip H. Howe
Keyword(s):  
Transcriptional Mapping

Transcriptional mapping of the amplified region encoding the dihydrofolate reductase-thymidylate synthase of Leishmania major reveals a high density of transcripts, including overlapping and antisense RNAs

1989 ◽  
Vol 9 (9) ◽  
pp. 3959-3972
Author(s):  
G M Kapler ◽  
S M Beverley
Keyword(s):  
Dihydrofolate Reductase ◽  
Thymidylate Synthase ◽  
Leishmania Major ◽  
Protozoan Parasite ◽  
Circular Dna ◽  
Antisense Rnas ◽  
Protein Levels ◽  
Polycistronic Transcription ◽  
Transcriptional Mapping ◽  
R Region

We have examined the transcriptional organization of the R region of the protozoan parasite Leishmania major. This region encodes the bifunctional enzyme dihydrofolate reductase-thymidylate synthase (DHFR-TS) and is frequently amplified as a 30-kilobase (kb) extrachromosomal circular DNA in methotrexate-resistant lines. Northern (RNA) blot analysis shows that the R region encodes at least 10 stable cytoplasmic polysomal poly(A)+ RNAs, ranging in size from 1.7 to 13 kb and including the 3.2-kb DHFR-TS mRNA. Transcriptional mapping reveals that these RNAs are closely spaced and collectively cover more than 95% of the 30-kb amplified R region. The organization is complex, including several overlapping RNAs 3' of DHFR-TS and two examples of antisense RNAs 5' of DHFR-TS. The R region RNAs can be grouped into two empirical domains, with eight contiguous RNAs transcribed in the same direction as that of DHFR-TS and two contiguous RNAs transcribed in the orientation opposite to that of DHFR-TS. The two 5'-most RNAs of the DHFR-TS-containing domain overlap the RNAs transcribed from the opposite strand. These data are relevant to models of transcription, including recent studies suggesting polycistronic transcription in trypanosomatids. The abundance of R region RNAs increases uniformly 10- to 15-fold in the amplified R1000-3 line relative to the wild type, and no new RNAs were observed. This suggests that all elements required in cis for DHFR-TS expression are contained within the 30-kb circular DNA. Quantitative analysis reveals that the steady-state DHFR-TS mRNA and protein levels are not growth phase regulated, unlike the monofunctional mouse DHFR. DHFR-TS is developmentally regulated, however, declining about fivefold in lesion amastigotes relative to promastigotes.

Molecular Cloning and Transcriptional Mapping of the Mouse Cellular Retinoic Acid-Binding Protein Gene

1990 ◽  
Vol 9 (7) ◽  
pp. 471-478 ◽  
Author(s):  
LI-NA WEI ◽  
JEN-LAN TSAO ◽  
YA-SHU CHU ◽  
LUCIE JEANNOTTE ◽  
M. CHI NGUYEN-HUU
Keyword(s):  
Retinoic Acid ◽  
Molecular Cloning ◽  
Protein Gene ◽  
Binding Protein ◽  
Acid Binding Protein ◽  
Transcriptional Mapping ◽  
Binding Protein Gene

Transcriptional mapping and genomic analysis of the cardiac atria and ventricles

2002 ◽  
Vol 12 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Xiao-Song Zhao ◽  
Teresa D. Gallardo ◽  
Ling Lin ◽  
Jeoffrey J. Schageman ◽  
Ralph V. Shohet
Keyword(s):  
Genomic Analysis ◽  
Myocyte Enhancer Factor 2 ◽  
Noncoding Sequences ◽  
Transcriptional Mapping ◽  
Evolutionarily Conserved ◽  
Conserved Noncoding Sequences ◽  
Detailed Evaluation ◽  
Development Structure ◽  
Enhancer Factor

The atria and ventricles of the heart have distinct development, structure, and physiology. However, only a few of the genes that underlie the differences between these tissues are known. We used a murine cardiac cDNA microarray to identify genes differentially expressed in the atria and ventricles. The reliability of these findings is supported by highly concordant repetition of hybridization, recognition of previously known atrial and ventricular isoforms of contractile proteins, and confirmation of results by quantitative PCR and in situ hybridization. We examined the most differentially regulated genes for evolutionarily conserved noncoding sequences and found that atrial-expressed genes have more predicted myocyte enhancer factor-2 (MEF2) binding sites than ventricle-predominant genes. We confirmed that messages for MEF2 family members are more abundant in the atria, as are their protein products. Moreover, the activity of a transgenic reporter construct for MEF2 activity is preferentially upregulated in the atria in response to hypertrophic stimuli. This study provides a greater understanding of the molecular differences between atria and ventricles and establishes the framework for an anatomically detailed evaluation of cardiac transcriptional regulation.

Physical and Transcriptional Mapping of the 17p13.3 Region That Is Frequently Deleted in Human Cancer

Genomics ◽  
2000 ◽  
Vol 70 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Céline Hoff ◽  
Peter Seranski ◽  
Jan Mollenhauer ◽  
Bernhard Korn ◽  
Tanja Detzel ◽  
...  
Keyword(s):  
Human Cancer ◽  
Transcriptional Mapping
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