scholarly journals Long non-coding RNA HCP5 functions as a sponge of miR-29b-3p and promotes cell growth and metastasis in hepatocellular carcinoma through upregulating DNMT3A

Aging ◽  
2021 ◽  
Author(s):  
Yongping Zhou ◽  
Kuan Li ◽  
Tu Dai ◽  
Hong Wang ◽  
Zhiyuan Hua ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Growth ◽  
Non Coding Rna ◽  
Long Non Coding Rna

scholarly journals Long non-coding RNA XIST promotes cell growth by regulating miR-139-5p/PDK1/AKT axis in hepatocellular carcinoma

Tumor Biology ◽  
2017 ◽  
Vol 39 (2) ◽  
pp. 101042831769099 ◽  
Author(s):  
Yichao Mo ◽  
Yaoyong Lu ◽  
Peng Wang ◽  
Simin Huang ◽  
Longguang He ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Growth ◽  
Cell Cycle Progression ◽  
Carcinoma Cell ◽  
Xist Expression ◽  
Cycle Progression ◽  
Non Coding Rna ◽  
Direct Target ◽  
Hepatocellular Carcinoma Cell ◽  
Long Non Coding Rna

Abnormal expression of long non-coding RNA often contributes to unrestricted growth of cancer cells. Long non-coding RNA XIST expression is upregulated in several cancers; however, its modulatory mechanisms have not been reported in hepatocellular carcinoma. In this study, we found that XIST expression was significantly increased in hepatocellular carcinoma tissues and cell lines. XIST promoted cell cycle progression from the G1 phase to the S phase and protected cells from apoptosis, which contributed to hepatocellular carcinoma cell growth. In addition, we revealed that there was reciprocal repression between XIST and miR-139-5p. PDK1 was identified as a direct target of miR-139-5p. We proposed that XIST was responsible for hepatocellular carcinoma cell proliferation, and XIST exerted its function through the miR-139-5p/PDK1 axis.

scholarly journals Long Non-Coding RNA HCP5 Functions as A Sponger of miR-29b-3p and Promotes Cell Growth and Metastasis in Hepatocellular Carcinoma Through Upregulating DNMT3A

2020 ◽  
Author(s):  
Yongping Zhou ◽  
Kuan Li ◽  
Tu Dai ◽  
Hong Wang ◽  
Zhiyuan Hua ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Flow Cytometry ◽  
Cell Growth ◽  
Dna Methyltransferase ◽  
Luciferase Assay ◽  
Migration And Invasion ◽  
Regulatory Factor ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Background: Multiple researches have revealed that long non-coding RNA (lncRNAs) served as regulatory factor in modulating tumorigenesis of hepatocellular carcinoma (HCC). In this study, we demonstrated that lncRNA HCP5 was a tumor promoting factor of HCC, and the molecular mechanism of HCP5 has been elucidated at length.Methods: BrdU, transwell assay and flow cytometry were exerted to estimate cell growth, metastasis, invasion and apoptosis of HCC in vitro. Animal studies were performed the effect of HCP5 in vivo. Furthermore, we identified that miR-29b-3p interacted with HCP5 or DNA methyltransferase 3A (DNMT3A) using dual luciferase assay. qRT-PCR, western blot, BrdU, tanswell and flow cytometry assays were used to investigate the correlation of HCP5/miR-29b-3p/DNMT3A axis. Results: In present research, we elaborated that HCP5 was overexpressed in HCC tissues and cell lines, and this phenomenon was even obvious in metastatic and recurrent cases. Knockdown of HCP5 significantly alleviated cell growth, metastasis and invasion through preventing apoptosis and activating EMT progress. Moreover, miR-29b-3p has been identified as a negatively regulatory target gene of HCP5, which served as tumor suppressor of HCC to prevent cell proliferation, migration and invasion. Subsequently, DNMT3A was found as a downstream regulatory factor of miR-29b-3p, of which study found that a participated element of HCC progression by activating AKT phosphorylation.Conclusion: Generally, our study elucidated for the first time that HCP5 plays a crucial role in HCC via HCP5/ miR-29b-3p/DNMT3A/AKT axis and demonstrated a novel diagnostic and therapeutic strategy with potentiality.

scholarly journals Long non-coding RNA-based signature for predicting prognosis of hepatocellular carcinoma

Bioengineered ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 673-681
Author(s):  
Jie Cao ◽  
Lili Wu ◽  
Xin Lei ◽  
Keqing Shi ◽  
Liang Shi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Non Coding Rna ◽  
Long Non Coding Rna
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