scholarly journals Racemization in cataractous lens from diabetic and aging individuals: analysis of Asp 58 residue in αA-crystallin

Aging ◽  
2021 ◽  
Author(s):  
Xiang-Jia Zhu ◽  
Ke-Ke Zhang ◽  
Wen-Wen He ◽  
Jiao Qi ◽  
Yi Lu
Keyword(s):  
Cataractous Lens

scholarly journals Reaction of tyrosine oxidation products with proteins of the lens

1968 ◽  
Vol 109 (2) ◽  
pp. 301-305 ◽  
Author(s):  
Antoinette Pirie
Keyword(s):  
Ascorbic Acid ◽  
Fluorescence Spectra ◽  
Ferrous Sulphate ◽  
Oxidation Products ◽  
Thiol Groups ◽  
Lens Proteins ◽  
Bovine Lens ◽  
Tyrosine Oxidation ◽  
Cataractous Lens

Oxidation of tyrosine in the presence of bovine lens proteins leads to the formation of brown or black melanoproteins. Both tyrosinase and the oxidizing system of ferrous sulphate–ascorbic acid–EDTA are effective. The fluorescence of the lens proteins is both altered and enhanced by the tyrosine-oxidizing systems. Their fluorescence spectra resemble those of urea-insoluble proteins of human cataractous lens and of 1,2-naphthaquinone–proteins of naphthalene cataract. The lens proteins lose their thiol groups and, in acid hydrolysates of treated β-and γ-crystallins, a substance has been detected chromatographically that behaves similarly to a compound formed when 3,4-dihydroxyphenylalanine (dopa) is oxidized by tyrosinase in the presence of cysteine. Analysis and behaviour of this substance from hydrolysates of lens proteins suggest that it is a compound of cysteine and dopa.

Capsulorrhexis Instrument Design for Use in Cataract Surgery

1999 ◽  
Author(s):  
Benjamin Turner ◽  
Shamsul Sulaiman ◽  
R. M. Pidaparti ◽  
S. Valluri
Keyword(s):  
Cataract Surgery ◽  
Instrument Design ◽  
Anterior Capsule ◽  
Cataractous Lens

Abstract A cataract develops when the lens of the eye becomes opaque, clouding one’s vision. This can be corrected by cataract surgery, where the opaque lens is removed and replaced with an artificial lens. During this surgery, it is necessary after entering the eye to cut the membrane covering the anterior capsule (Fig. 1) to allow access to the cataractous lens. The cutting of this membrane is known as capsulorrhexis.

Postoperative Complications after Phacoemulsification and Implantation of Foldable Acrylic Intraocular Lenses in Dogs

2021 ◽  
Author(s):  
Babita Das ◽  
Apra Shahi ◽  
Vishnu Pratap Chandrapuria ◽  
Shobha Jawre ◽  
Madhu Swamy ◽  
...  
Keyword(s):  
Postoperative Complications ◽  
Cataract Surgery ◽  
Postoperative Complication ◽  
Corneal Opacity ◽  
Intraocular Lenses ◽  
Major Postoperative Complication ◽  
Ophthalmic Pathology ◽  
Cataractous Lens ◽  
Operative Complications ◽  
Post Operative Complications

Background: Despite significant advances in canine cataract surgery over the years, many post-operative complications persist and reduces the success rate of phacoemulsification procedure. The aim of current study was to evaluate post-operative complications encountered till 90 days after bimanual phacoemulsification with implantation of different acrylic Intraocular Lenses.Methods: The study was conducted on 24 canine eyes. All the clinical cases were subjected to detailed ocular, ultrasonography and neuro-ophthalmic tests for ascertaining cataract and associated neuro-ophthalmic pathology. Dogs were divided in four groups with 6 eyes in each group and subjected to phacoemulsification procedure for removal of cataractous lens and implanted with square edge or round edge hydrophilic or hydrophobicintra ocular lenses.Result: Statistically non-significant (p£0.05) variations were found for all the post- operative complications among the groups. Within the groups initially higher values were recorded and on subsequent days a declining trend of varying degrees were observed. The corneal opacity was a major postoperative complication leading to failure to achieve vision with other coinciding neuro-ophthalmic conditions.

Measuring the cataractous lens

2015 ◽  
Vol 41 (9) ◽  
pp. 1875-1879 ◽  
Author(s):  
John H. Shammas ◽  
Maya C. Shammas
Keyword(s):  
Cataractous Lens

Crystallin composition of human cataractous lens may be modulated by protein glycation

1996 ◽  
Vol 234 (S1) ◽  
pp. S232-S238 ◽  
Author(s):  
José Ramalho ◽  
Carla Marques ◽  
Paulo Pereira ◽  
M. Carolina Mota
Keyword(s):  
Protein Glycation ◽  
Cataractous Lens

Rescue of developmental lens abnormalities in chimaeras of noncataractous and congenital cataractous mice

Development ◽  
1987 ◽  
Vol 99 (4) ◽  
pp. 473-480
Author(s):  
A.L. Muggleton-Harris ◽  
K. Hardy ◽  
N. Higbee
Keyword(s):  
Medical Research ◽  
Congenital Cataract ◽  
Biochemical Analysis ◽  
Growth Regulation ◽  
Cellular Level ◽  
Model System ◽  
Mouse Mutant ◽  
Phenotypic Characteristics ◽  
Cataractous Lens ◽  
Mouse Lens

In the study of the lens of a congenital cataractous mouse mutant (CAT), it has been shown that a loss of growth regulation at the cellular level causes gross lens abnormalities. The phenotypic characteristics of the cataractous mouse lens are similar to those seen in human congenital cataract and thus serves as a model system for medical research. In this present investigation, we have demonstrated that the abnormalities of the congenital cataractous lens can be rescued by forming chimaeras between DBA/2 (a noncataractous strain of mouse) and the CAT mutant. This report describes the histological, cellular and biochemical analysis of the resultant chimaeric eyes, and discusses possible mechanisms by which these results were achieved.

Electrolyte composition of experimental galactose cataracts

1959 ◽  
Vol 197 (1) ◽  
pp. 85-86 ◽  
Author(s):  
Joseph C. Hill ◽  
Franklin W. Heggeness
Keyword(s):  
Water Content ◽  
Dietary Calcium ◽  
Electrolyte Composition ◽  
Control Group ◽  
Deficient Diet ◽  
Organic Solids ◽  
Low Calcium ◽  
Cataractous Lens ◽  
Normal Rat

The composition of galactose-induced cataractous lens differs from that of the normal rat lens. Weight is decreased due to loss of both water and organic solids. Within a few days of maturation of the cataracts the sodium and calcium concentrations are increased and potassium is virtually absent from the lens. No differences were found in the water content or electrolyte composition of other nonvascular tissues such as tendon, cornea or cartilage in rats maintained on a high galactose intake as compared to the control group of rats on a glucose diet. Modifications of the diet by the inclusion of sulfasuxidine or by sulfasuxide plus low calcium do not modify the general electrolyte pattern of the cataractous lens. In rats ingesting a high galactose, calcium-deficient diet cataracts develop sooner than in the presence of adequate dietary calcium.

scholarly journals Synchrotron-based FTIR microspectroscopy of protein aggregation and lipids peroxidation changes in human cataractous lens epithelial cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Martin Kreuzer ◽  
Tanja Dučić ◽  
Marko Hawlina ◽  
Sofija Andjelic
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Protein Aggregation ◽  
Photon Flux ◽  
Lens Epithelial Cells ◽  
Ftir Microspectroscopy ◽  
Age Related ◽  
Cataractous Lens ◽  
Synchrotron Light ◽  
Common Environmental Factor

Abstract Cataract is the leading cause of blindness worldwide but the mechanisms involved in the process of cataractogenesis are not yet fully understood. Two most prevalent types of age-related cataracts are nuclear (N) and cortical (C) cataracts. A common environmental factor in most age-related cataracts is believed to be oxidative stress. The lens epithelium, the first physical and biological barrier in the lens, is build from lens epithelial cells (LECs). LECs are important for the maintenance of lens transparency as they control energy production, antioxidative mechanisms and biochemical transport for the whole lens. The purpose of this study is to characterize compounds in LECs originated from N and C cataracts, by using the synchrotron radiation-based Fourier Transform Infrared (SR-FTIR) microspectroscopy, in order to understand the functional importance of their different bio-macromolecules in cataractogenesis. We used the SR-FTIR microspectroscopy setup installed on the beamline MIRAS at the Spanish synchrotron light source ALBA, where measurements were set to achieve single cell resolution, with high spectral stability and high photon flux. The results showed that protein aggregation in form of fibrils was notably pronounced in LECs of N cataracts, while oxidative stress and the lipids peroxidation were more pronounced in LECs of C cataracts.

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