scholarly journals An aging and p53 related marker: HOXA5 promoter methylation negatively correlates with mRNA and protein expression in old age

Aging ◽  
2021 ◽  
Author(s):  
Laura-Kim Feiner ◽  
Sascha Tierling ◽  
Sebastian Holländer ◽  
Matthias Glanemann ◽  
Claudia Rubie
Keyword(s):  
Protein Expression ◽  
Old Age ◽  
Promoter Methylation ◽  
Mrna And Protein Expression

scholarly journals ABCG2 expression, function, and promoter methylation in human multiple myeloma

Blood ◽  
2006 ◽  
Vol 108 (12) ◽  
pp. 3881-3889 ◽  
Author(s):  
Joel G. Turner ◽  
Jana L. Gump ◽  
Chunchun Zhang ◽  
James M. Cook ◽  
Douglas Marchion ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Flow Cytometry ◽  
Drug Resistance ◽  
Protein Expression ◽  
Cell Lines ◽  
Promoter Methylation ◽  
Plasma Cells ◽  
Cancer Resistance ◽  
Response To Chemotherapy ◽  
Mrna And Protein Expression

AbstractWe investigated the role of the breast cancer resistance protein (BCRP/ABCG2) in drug resistance in multiple myeloma (MM). Human MM cell lines, and MM patient plasma cells isolated from bone marrow, were evaluated for ABCG2 mRNA expression by quantitative polymerase chain reaction (PCR) and ABCG2 protein, by Western blot analysis, immunofluorescence microscopy, and flow cytometry. ABCG2 function was determined by measuring topotecan and doxorubicin efflux using flow cytometry, in the presence and absence of the specific ABCG2 inhibitor, tryprostatin A. The methylation of the ABCG2 promoter was determined using bisulfite sequencing. We found that ABCG2 expression in myeloma cell lines increased after exposure to topotecan and doxorubicin, and was greater in logphase cells when compared with quiescent cells. Myeloma patients treated with topotecan had an increase in ABCG2 mRNA and protein expression after treatment with topotecan, and at relapse. Expression of ABCG2 is regulated, at least in part, by promoter methylation both in cell lines and in patient plasma cells. Demethylation of the promoter increased ABCG2 mRNA and protein expression. These findings suggest that ABCG2 is expressed and functional in human myeloma cells, regulated by promoter methylation, affected by cell density, up-regulated in response to chemotherapy, and may contribute to intrinsic drug resistance.

Regulation of MLH1 mRNA and protein expression by promoter methylation in primary colorectal cancer: a descriptive and prognostic cancer marker study

2013 ◽  
Vol 36 (5) ◽  
pp. 411-419 ◽  
Author(s):  
Lars Henrik Jensen ◽  
Anders Aamann Rasmussen ◽  
Lene Byriel ◽  
Hidekazu Kuramochi ◽  
Dorthe Gylling Crüger ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Protein Expression ◽  
Promoter Methylation ◽  
Primary Colorectal Cancer ◽  
Cancer Marker ◽  
Mrna And Protein Expression ◽  
Marker Study

ABCG2 Expression and Methylated Regulation in Human Multiple Myeloma Cell Lines

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5138-5138
Author(s):  
Shiqiang Qu ◽  
Chengcheng Fu ◽  
Li Yao ◽  
Guanghua Chen ◽  
Jiannong Cen ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Protein Expression ◽  
Quantitative Pcr ◽  
Cell Lines ◽  
Promoter Methylation ◽  
Melting Curve ◽  
Myeloma Cell ◽  
Sybr Green ◽  
Mrna And Protein Expression ◽  
Demethylation Agent

Abstract Objective: The expression and regulation of multidrug resistance protein ABCG2 in multiple myeloma (MM) is unclear, expression of ABCG2 mRNA and protein, promoter methylation regulation of ABCG2 in MM cell lines will be investigated. Methods After human MM cell lines 8226 AU266 and XG-7 were incubated in the demethylation agent 5’-AzaDC, the change of ABCG2 mRNA and protein expression were evaluated separately by using the Taqman probe-based real-time quantitative polymerase chain reaction(RQ-RT-PCR) and flow cytometry. The percentage of methylated alleles was assayed by SYBR green based methylation-specific quantitative PCR (MSQ-PCR). The melting curve was then analyzed to judge the specificity of the PCR product. Results The three MM cell lines have different expression level of ABCG2 mRNA(Table 1) and protein but all much higher than normal PBMCs and in different promoter methylation status. The highest level of ABCG2 mRNA and protein were detected in the 8266 cell lines and the promotor region is in unmethylation status. Expression in the U266 cell lines was moderate and the methylated percentage is 61.55%±1.98%. Expression in the XG-7 is the least, the methylated percentage is 78.40%±2.46%. ABCG2 expression increased after exposure to demethylation agent 5’-AzaDC to 2 fold in U266 and 3.5 fold in XG-7 cell lines. After MSQ-PCR, the melting curve was analyzed. The Tm value of methylated product and the unmethylated product were 77°C and 75.5°C separately. Conclution: ABCG2 mRNA and protein expression in 3 MM cell lines were much higher than that in normal peripheral blood mononuclear cells. Demethylation of the promotor increased ABCG2 mRNA and protein expression. So expression of ABCG2 is partially regulated by promoter methylation in myeloma cell lines. The cell line with the more ABCG2 expression may have more potential to effluence the chemotherapy agents. SYBR green I based fluorescence quantitative PCR can calculate the percentage of the methylated alleles in genome.

scholarly journals Inactivation of parkin by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769502 ◽  
Author(s):  
Haifeng Ni ◽  
Zhen Zhou ◽  
Bo Jiang ◽  
Xiaoyang Yuan ◽  
Xiaolin Cao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nasopharyngeal Carcinoma ◽  
Protein Expression ◽  
Mrna Expression ◽  
Genomic Instability ◽  
Promoter Methylation ◽  
Chain Reaction ◽  
Node Metastasis ◽  
Mrna And Protein Expression ◽  
Polymerase Chain

This study aimed to investigate the inactivation of the parkin gene by promoter methylation and its relationship with genome instability in nasopharyngeal carcinoma. Parkin was considered as a tumor suppressor gene in various types of cancers. However, its role in nasopharyngeal carcinoma is unexplored. Genomic instabilities were detected in nasopharyngeal carcinoma tissues by the random amplified polymorphic DNA. The methylation-specific polymerase chain reaction, semi-quantitative reverse transcription polymerase chain reaction, and immunohistochemical analysis were used to detect methylation and mRNA and protein expression of parkin in 54 cases of nasopharyngeal carcinoma tissues and 16 cases of normal nasopharyngeal epithelia tissues, and in 5 nasopharyngeal carcinoma cell lines (CNE1, CNE2, TWO3, C666, and HONE1) and 1 normal nasopharyngeal epithelia cell line (NP69). mRNA expression of parkin in CNE1 and CNE2 was analyzed before and after methyltransferase inhibitor 5-aza-2-deoxycytidine treatment. The relationship between promoter methylation and mRNA expression, demethylation and mRNA expression, and mRNA and protein expression of the gene and clinical factors and genomic instabilities were analyzed. The mRNA and protein expression levels were significantly reduced in 54 cases of human nasopharyngeal carcinoma compared with 16 cases of normal nasopharyngeal epithelia. Parkin-methylated cases showed significantly lower mRNA and protein expression levels compared with unmethylated cases. After 5-aza-2-deoxycytidine treatment, parkin mRNA expression was restored in CNE1 and CNE2; 92.59% (50/54) of nasopharyngeal carcinoma demonstrated genomic instability. Parkin is frequently inactivated by promoter methylation, and its mRNA and protein expression correlate with lymph node metastasis and genomic instability. Parkin deficiency probably promotes tumorigenesis in nasopharyngeal carcinoma.

scholarly journals TGF-β1 epigenetically modifies Thy-1 expression in primary lung fibroblasts

2015 ◽  
Vol 309 (9) ◽  
pp. C616-C626 ◽  
Author(s):  
Wendy A. Neveu ◽  
Stephen T. Mills ◽  
Bashar S. Staitieh ◽  
Viranuj Sueblinvong
Keyword(s):  
Protein Expression ◽  
Promoter Methylation ◽  
Dna Methyltransferase ◽  
Transforming Growth Factor ◽  
Fiber Formation ◽  
Lung Fibroblasts ◽  
Col1a1 Gene ◽  
Gene And Protein Expression ◽  
Mrna And Protein Expression ◽  
Tgf Β1

Idiopathic pulmonary fibrosis is a progressive lung disease that increases in incidence with age. We identified a profibrotic lung phenotype in aging mice characterized by an increase in the number of fibroblasts lacking the expression of thymocyte differentiation antigen 1 (Thy-1) and an increase in transforming growth factor (TGF)-β1 expression. It has been shown that Thy-1 expression can be epigenetically modified. Lung fibroblasts (PLFs) were treated with TGF-β1 ± DNA methyltransferase (DNMT) inhibitor 5-aza-2′-deoxycytidine (5-AZA) and analyzed for Thy-1 gene and protein expression, DNMT protein expression, and activity. α-Smooth muscle actin (α-SMA) and collagen type 1 (Col1A1) gene and protein expression was assessed. PLFs were transfected with DNMT1 silencing RNA ± TGF-β1. TGF-β1 inhibited Thy-1 gene and protein expression in PLFs, and cotreatment with 5-AZA ameliorated this effect and appeared to inhibit DNMT1 activation. TGF-β1 induced Thy-1 promoter methylation as assessed by quantitative methyl PCR. Treatment with 5-AZA attenuated TGF-β1-induced Col1A1 gene and protein expression and α-SMA gene expression (but not α-SMA protein expression). Inhibiting DNMT1 with silencing RNA attenuated TGF-β1-induced DNMT activity and its downstream suppression of Thy-1 mRNA and protein expression as well as inhibited α-SMA mRNA and Col1A1 mRNA and protein expression, and showed a decreased trend in Thy-1 promoter methylation. Immunofluorescence for α-SMA suggested that 5-AZA inhibited stress fiber formation. These findings suggest that TGF-β1 epigenetically regulates lung fibroblast phenotype through methylation of the Thy-1 promoter. Targeted inhibition of DNMT in the right clinical context might prevent fibroblast to myofibroblast transdifferentiation and collagen deposition, which in turn could prevent fibrogenesis in the lung and other organs.

PTPIP51 mRNA and protein expression in tissue microarrays and promoter methylation of benign prostate hyperplasia and prostate carcinoma

The Prostate ◽  
2009 ◽  
Vol 69 (16) ◽  
pp. 1751-1762 ◽  
Author(s):  
Philipp Koch ◽  
Meike Petri ◽  
Agnieszka Paradowska ◽  
Albrecht Stenzinger ◽  
Klaus Sturm ◽  
...  
Keyword(s):  
Protein Expression ◽  
Prostate Carcinoma ◽  
Promoter Methylation ◽  
Benign Prostate Hyperplasia ◽  
Tissue Microarrays ◽  
Prostate Hyperplasia ◽  
Mrna And Protein Expression ◽  
Benign Prostate

Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients

2000 ◽  
Vol 111 (4) ◽  
pp. 1118-1121 ◽  
Author(s):  
A. Bellahcene ◽  
I. Van Riet ◽  
C. de Greef ◽  
N. Antoine ◽  
M. F. Young ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Protein Expression ◽  
Cell Lines ◽  
Myeloma Cell ◽  
Bone Sialoprotein ◽  
Multiple Myeloma Cell ◽  
Human Multiple Myeloma ◽  
Mrna And Protein Expression

Changes in mRNA and Protein Expression of Antioxidant Enzymes in Leukocytes of Children with Bronchial Asthma after Interval Hypoxic Training

2012 ◽  
Vol 3 (2) ◽  
pp. 125-134
Author(s):  
Klaudia V. Nesvitaylova ◽  
Olga A. Gonchar ◽  
Tatyana I. Drevitskaya ◽  
Ludmila P. Arabskaya ◽  
Mikhail M. Steshenko ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Bronchial Asthma ◽  
Hypoxic Training ◽  
Mrna And Protein Expression
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