scholarly journals Integrative analysis of exosomal microRNA-149-5p in lung adenocarcinoma

Aging ◽  
2021 ◽  
Author(s):  
Wen Tian ◽  
He Yang ◽  
Baosen Zhou
Keyword(s):  
Lung Adenocarcinoma ◽  
Integrative Analysis ◽  
Exosomal Microrna

scholarly journals Unique MicroRNA and mRNA Interactions in EGFR-Mutated Lung Adenocarcinoma

2018 ◽  
Vol 7 (11) ◽  
pp. 419 ◽  
Author(s):  
Sophia Subat ◽  
Kentaro Inamura ◽  
Hironori Ninomiya ◽  
Hiroko Nagano ◽  
Sakae Okumura ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Mrna Expression ◽  
Regulatory Network ◽  
Egfr Mutation ◽  
Expression Profiles ◽  
Integrative Analysis ◽  
Future Research ◽  
Mutation Status ◽  
Egfr Mutated ◽  
Insight Into

The EGFR gene was one of the first molecules to be selected for targeted gene therapy. EGFR-mutated lung adenocarcinoma, which is responsive to EGFR inhibitors, is characterized by a distinct oncogenic pathway in which unique microRNA (miRNA)–mRNA interactions have been observed. However, little information is available about the miRNA–mRNA regulatory network involved. Both miRNA and mRNA expression profiles were investigated using microarrays in 155 surgically resected specimens of lung adenocarcinoma with a known EGFR mutation status (52 mutated and 103 wild-type cases). An integrative analysis of the data was performed to identify the unique miRNA–mRNA regulatory network in EGFR-mutated lung adenocarcinoma. Expression profiling of miRNAs and mRNAs yielded characteristic miRNA/mRNA signatures (19 miRNAs/431 mRNAs) in EGFR-mutated lung adenocarcinoma. Five of the 19 miRNAs were previously listed as EGFR-mutation-specific miRNAs (i.e., miR-532-3p, miR-500a-3p, miR-224-5p, miR-502-3p, and miR-532-5p). An integrative analysis of miRNA and mRNA expression revealed a refined list of putative miRNA–mRNA interactions, of which 63 were potentially involved in EGFR-mutated tumors. Network structural analysis provided a comprehensive view of the complex miRNA–mRNA interactions in EGFR-mutated lung adenocarcinoma, including DUSP4 and MUC4 axes. Overall, this observational study provides insight into the unique miRNA–mRNA regulatory network present in EGFR-mutated tumors. Our findings, if validated, would inform future research examining the interplay of miRNAs and mRNAs in EGFR-mutated lung adenocarcinoma.

scholarly journals Exosomal MicroRNA Expression Profiling in Patients with Lung Adenocarcinoma-associated Malignant Pleural Effusion

2018 ◽  
Vol 38 (12) ◽  
pp. 6707-6714 ◽  
Author(s):  
HIROYUKI TAMIYA ◽  
AKIHISA MITANI ◽  
AKIRA SAITO ◽  
TARO ISHIMORI ◽  
MINAKO SAITO ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Lung Adenocarcinoma ◽  
Expression Profiling ◽  
Malignant Pleural Effusion ◽  
Microrna Expression ◽  
Exosomal Microrna

Integrative Analysis Reveals Enhanced Regulatory Effects of Human Long Intergenic Non-Coding RNAs in Lung Adenocarcinoma

2015 ◽  
Vol 42 (8) ◽  
pp. 423-436 ◽  
Author(s):  
You Zhou ◽  
Kai Wu ◽  
Jianping Jiang ◽  
Jinfei Huang ◽  
Peiwei Zhang ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Integrative Analysis ◽  
Non Coding Rnas ◽  
Regulatory Effects

scholarly journals Integrative analysis of exosomal microRNA-149-5p in lung adenocarcinoma

2020 ◽  
Author(s):  
Wen Tian ◽  
He Yang ◽  
Baosen Zhou
Keyword(s):  
Lung Adenocarcinoma ◽  
Tumor Cells ◽  
Target Genes ◽  
R Package ◽  
Negative Regulator ◽  
Poor Survival ◽  
Exosomal Mirnas ◽  
Independent Risk Factors ◽  
And Migration ◽  
Exosomal Microrna

Abstract Background Exosomes play an important role in the regulation of various processes in the tumor microenvironment. In this study, we explored the mechanisms of exosomal miR-149-5p in the pathogenesis of lung adenocarcinoma. Methods Raw GEO data were downloaded and normalized using the R package. Exosomal miRNAs that were found to be significantly expressed were subjected to WGCNA co-expression network analysis. The proliferation, migration and apoptotic abilities of tumor cells were assessed by the MTS, Transwell and apoptosis assays. Univariate and multivariate analyses were performed to identify the independent factors of target genes. Results Results showed that exosomal miR-149-5p was enriched in peripheral serum and tumor cells. The upregulation of exosomal miR-149-5p promoted the growth and migration of tumor cells, and inhibited apoptosis of tumor cells. Notably, target genes of exosomal miR-149-5p, AMOTL2 and MSRB3, were significantly downregulated in lung adenocarcinoma and thus may be considered as independent risk factors of poor survival. In TCGA-LUAD cohort, miR-149-5p was found to be a negative regulator of AMOTL2 and MSRB3 genes.Conclusion Collectively, these results provide novel insights for further mechanistic studies on lung adenocarcinoma.

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