scholarly journals DUSP4 directly deubiquitinates and stabilizes Smad4 protein, promoting proliferation and metastasis of colorectal cancer cells

Aging ◽  
2020 ◽  
Vol 12 (17) ◽  
pp. 17634-17646
Author(s):  
Weifeng Xu ◽  
Beibei Chen ◽  
Dianshan Ke ◽  
Xiaobing Chen
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Smad4 Protein ◽  
Colorectal Cancer Cells ◽  
Proliferation And Metastasis

TRIP6, as a target of miR-7, regulates the proliferation and metastasis of colorectal cancer cells

2019 ◽  
Vol 514 (1) ◽  
pp. 231-238 ◽  
Author(s):  
Yiting Ling ◽  
Chenxi Cao ◽  
Sengjuan Li ◽  
Min Qiu ◽  
Guixing Shen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Colorectal Cancer Cells ◽  
Proliferation And Metastasis

scholarly journals SIRT2, a direct target of miR‐212‐5p, suppresses the proliferation and metastasis of colorectal cancer cells

2020 ◽  
Vol 24 (17) ◽  
pp. 9985-9998
Author(s):  
Feng Du ◽  
Zhijun Li ◽  
Guohua Zhang ◽  
Si Shaoyan ◽  
Dejun Geng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Colorectal Cancer Cells ◽  
Proliferation And Metastasis ◽  
Direct Target

scholarly journals Calebin A Potentiates the Effect of 5-FU and TNF-β (Lymphotoxin α) against Human Colorectal Cancer Cells: Potential Role of NF-κB

2020 ◽  
Vol 21 (7) ◽  
pp. 2393 ◽  
Author(s):  
Constanze Buhrmann ◽  
Ajaikumar Kunnumakkara ◽  
Bastian Popper ◽  
Muhammed Majeed ◽  
Bharat Aggarwal ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Nuclear Translocation ◽  
Chemotherapeutic Agents ◽  
Colorectal Cancer Cells ◽  
Tumor Environment ◽  
Proliferation And Metastasis ◽  
Dtt Dithiothreitol ◽  
Apoptotic Effects ◽  
Lymphotoxin Α

Objective: The majority of chemotherapeutic agents stimulate NF-κB signaling that mediates cell survival, proliferation and metastasis. The natural turmeric non-curcuminoid derivate Calebin A has been shown to suppress cell growth, invasion and colony formation in colorectal cancer cells (CRC) by suppression of NF-κB signaling. Therefore, we hypothesized here that Calebin A might chemosensitize the TNF-β-treated tumor cells and potentiates the effect of 5-Fluorouracil (5-FU) in advanced CRC. Materials and Methods: CRC cells (HCT116) and their clonogenic 5-FU chemoresistant counterparts (HCT116R) were cultured in monolayer or alginate-based 3D tumor environment culture and were treated with/without Calebin A, TNF-β, 5-FU, BMS-345541 and DTT (dithiothreitol). Results: The results showed that TNF-β increased proliferation, invasion and resistance to apoptosis in chemoresistant CRC cells. Pretreatment with Calebin A significantly chemosensitized HCT116R to 5-FU and inhibited the TNF-β-induced enhanced efforts for survival, invasion and anti-apoptotic effects. We found further that Calebin A significantly suppressed TNF-β-induced phosphorylation and nuclear translocation of p65-NF-κB, similar to BMS-345541 (specific IKK inhibitor) and NF-κB-induced tumor-promoting biomarkers (NF-κB, β1-Integrin, MMP-9, CXCR4, Ki67). This was associated with increased apoptosis in HCT116 and HCT116R cells. Furthermore, blocking of p65-NF-κB stimulation by Calebin A was imparted through the downmodulation of p65-NF-κB binding to the DNA and this suppression was turned by DTT. Conclusion: Our findings indicate, for the first time, that Calebin A chemosensitizes human CRC cells to chemotherapy by targeting of the p65-NF-κB signaling pathway.

Sign in / Sign up

Export Citation Format

Share Document