scholarly journals FGF2-induced PI3K/Akt signaling evokes greater proliferation and adipogenic differentiation of human adipose stem cells from breast than from abdomen or thigh

Aging ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 14830-14848
Author(s):  
Guan-Ming Lu ◽  
Yong-Xian Rong ◽  
Zhi-Jie Liang ◽  
Dong-Lin Hunag ◽  
Fang-Xiao Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipogenic Differentiation ◽  
Akt Signaling ◽  
Adipose Stem Cells ◽  
Human Adipose Stem Cells

scholarly journals Glycoengineering Human Neural and Adipose Stem Cells with Novel Thiol-Modified N-Acetylmannosamine (ManNAc) Analogs

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 377
Author(s):  
Jian Du ◽  
Christian Agatemor ◽  
Christopher T. Saeui ◽  
Rahul Bhattacharya ◽  
Xiaofeng Jia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wnt Signaling ◽  
Embryoid Body ◽  
Adipogenic Differentiation ◽  
Adipose Stem Cells ◽  
Human Stem Cells ◽  
Human Neural Stem Cells ◽  
Glial Lineage ◽  
Metabolic Glycoengineering ◽  
Human Adipose Stem Cells

This report describes novel thiol-modified N-acetylmannosamine (ManNAc) analogs that extend metabolic glycoengineering (MGE) applications of Ac5ManNTGc, a non-natural monosaccharide that metabolically installs the thio-glycolyl of sialic acid into human glycoconjugates. We previously found that Ac5ManNTGc elicited non-canonical activation of Wnt signaling in human embryoid body derived (hEBD) cells but only in the presence of a high affinity, chemically compatible scaffold. Our new analogs Ac5ManNTProp and Ac5ManNTBut overcome the requirement for a complementary scaffold by displaying thiol groups on longer, N-acyl linker arms, thereby presumably increasing their ability to interact and crosslink with surrounding thiols. These new analogs showed increased potency in human neural stem cells (hNSCs) and human adipose stem cells (hASCs). In the hNSCs, Ac5ManNTProp upregulated biochemical endpoints consistent with Wnt signaling in the absence of a thiol-reactive scaffold. In the hASCs, both Ac5ManNTProp and Ac5ManNTBut suppressed adipogenic differentiation, with Ac5ManNTBut providing a more potent response, and they did not interfere with differentiation to a glial lineage (Schwann cells). These results expand the horizon for using MGE in regenerative medicine by providing new tools (Ac5ManNTProp and Ac5ManNTBut) for manipulating human stem cells.

scholarly journals Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1939
Author(s):  
Katharina M. Prautsch ◽  
Alexander Schmidt ◽  
Viola Paradiso ◽  
Dirk J. Schaefer ◽  
Raphael Guzman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Neurotrophic Factor ◽  
Axonal Growth ◽  
Cellular Level ◽  
Adipose Stem Cells ◽  
Transcriptional Analysis ◽  
Axonal Outgrowth ◽  
Human Adipose Stem Cells

We report on a potential strategy involving the exogenous neurotrophic factors (NTF) for enhancing the neurotrophic capacity of human adipose stem cells (ASC) in vitro. For this, ASC were stimulated for three days using NTF, i.e., nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT3), NT4, glial cell-derived neurotrophic factor (GDNF), and ciliary neurotrophic factor (CNTF). The resulting conditioned medium (CM) as well as individual NTF exhibited distinct effects on axonal outgrowth from dorsal root ganglion (DRG) explants. In particular, CM derived from NT3-stimulated ASC (CM-NT3-ASC) promoted robust axonal outgrowth. Subsequent transcriptional analysis of DRG cultures in response to CM-NT3-ASC displayed significant upregulation of STAT-3 and GAP-43. In addition, phosphoproteomic analysis of NT3-stimulated ASC revealed significant changes in the phosphorylation state of different proteins that are involved in cytokine release, growth factors signaling, stem cell maintenance, and differentiation. Furthermore, DRG cultures treated with CM-NT3-ASC exhibited significant changes in the phosphorylation levels of proteins involved in tubulin and actin cytoskeletal pathways, which are crucial for axonal growth and elongation. Thus, the results obtained at the transcriptional, proteomic, and cellular level reveal significant changes in the neurotrophic capacity of ASC following NT3 stimulation and provide new options for improving the axonal growth-promoting potential of ASC in vitro.

scholarly journals Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

PLoS ONE ◽  
2019 ◽  
Vol 14 (10) ◽  
pp. e0222597 ◽  
Author(s):  
Sara Freitas-Ribeiro ◽  
Andreia Filipa Carvalho ◽  
Marina Costa ◽  
Mariana Teixeira Cerqueira ◽  
Alexandra Pinto Marques ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Cell Sheets ◽  
Hypothermic Preservation ◽  
Human Adipose Stem Cells

Exogenously added BMP-6, BMP-7 and VEGF may not enhance the osteogenic differentiation of human adipose stem cells

2013 ◽  
Vol 31 (5) ◽  
pp. 141-153 ◽  
Author(s):  
Laura Kyllönen ◽  
Suvi Haimi ◽  
Janne Säkkinen ◽  
Hannu Kuokkanen ◽  
Bettina Mannerström ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Adipose Stem Cells ◽  
Human Adipose Stem Cells

scholarly journals The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202740 ◽  
Author(s):  
Miina Ojansivu ◽  
Ayush Mishra ◽  
Sari Vanhatupa ◽  
Miia Juntunen ◽  
Antonina Larionova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Borosilicate Glasses ◽  
Glass Dissolution ◽  
Human Adipose Stem Cells

220 CpG METHYLATION PROFILE OF ADIPOGENIC AND ENDOTHELIAL GENE PROMOTERS IN HUMAN ADIPOSE STEM CELLS SUGGESTS A RESTRICTIVE ENDOTHELIAL DIFFERENTIATION POTENTIAL

2007 ◽  
Vol 19 (1) ◽  
pp. 227
Author(s):  
A. C. Boquest ◽  
A. Noer ◽  
A. L. Sørensen ◽  
K. Vekterud ◽  
P. Collas
Keyword(s):  
Stem Cells ◽  
Adipogenic Differentiation ◽  
Cpg Methylation ◽  
Methylation Profile ◽  
Adipose Stem Cells ◽  
Endothelial Differentiation ◽  
Sequencing Analysis ◽  
Differentiation Potential ◽  
Undifferentiated Cells ◽  
Endothelial Lineage

Mesenchymal stem cells (MSCs) have received intense research interest due to their perceived potential application in regenerative medicine; nevertheless, MSCs are primarily restricted to form mesodermal cell types. Adipose stem cells (ASCs) with a CD34+ CD105+ CD45– CD31– immunophenotype can be obtained in an uncultured state with high purity from the stromal vascular fraction of human liposuction material (Boquest et al. 2005 Mol. Biol. Cell 16, 1131–1141). While ASCs differentiate readily into adipocytes, their endothelial lineage commitment has been scarcely reported, and controversy remains regarding ASC contribution to vascularization. To address the epigenetic commitment of ASCs to adipogenic and endothelial lineages, we carried out a bisulfite sequencing analysis of CpG methylation in the promoters of adipogenic (LEP, PPARG2, FABP4, LPL), endothelial (CD31, CD144), and myogenic (MYOG) genes in freshly isolated and in clonal ASC cultures in relation to gene expression and differentiation potential. Uncultured ASCs display mosaic hypomethylation of adipogenic promoters, in contrast to MYOG, CD31, or CD144 which are methylated (Noer et al. 2006 Mol. Biol. Cell 17, in press). Nevertheless, CpG methylation does not reflect transcriptional status of these genes in undifferentiated cells. Culture and adipogenic differentiation of ASCs maintains the hypomethylated profile of adipogenic promoters and the hypermethylation of non-adipogenic promoters. Endothelial stimulation of ASCs in methylcellulose elicits tubule-like networks, up-regulation of CD31 and CD144, and restrictive induction of a CD31+ CD144+ immunophenotype. Discrete and lineage-specific changes in CpG methylation in the CD31 and CD144 promoters take place but no global demethylation that marks endothelial cells occurs. Promoters not involved in endothelial differentiation retain a methylation profile characteristic of undifferentiated cells. Hypermethylation of CD31 and CD144 suggests a restricted commitment of ASCs to the endothelial lineage. This contrasts with hypomethylation of adipogenic promoters which reflects a propensity toward adipogenic differentiation. Despite the up-regulation of lineage-specific transcripts, overall maintenance of promoter methylation after adipogenic, osteogenic, and endothelial differentiation suggests the maintenance of an epigenetic signature characteristic of undifferentiated cells. Analysis of CpG methylation at lineage-specific promoters should provide a robust assessment of epigenetic commitment of stem cells to a specific lineage.

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