scholarly journals Mitochondrial nucleoid remodeling and biogenesis are regulated by the p53-p21WAF1-PKCζ pathway in p16INK4a-silenced cells

Aging ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 6700-6732 ◽  
Author(s):  
Yun Yeong Lee ◽  
Yeon Seung Choi ◽  
Do Wan Kim ◽  
Jae Youn Cheong ◽  
Kye Yong Song ◽  
...  
Keyword(s):  
Mitochondrial Nucleoid

scholarly journals Mitochondrial nucleoid visualization in isolated pancreatic β-cells

2014 ◽  
Vol 1837 ◽  
pp. e29-e30
Author(s):  
Tomas Olejar ◽  
Lukas Alan ◽  
Monika Cahova ◽  
Zuzana Berkova ◽  
Frantisek Saudek ◽  
...  
Keyword(s):  
Β Cells ◽  
Pancreatic Β Cells ◽  
Mitochondrial Nucleoid

scholarly journals The Mitochondrial Nucleoid: Integrating Mitochondrial DNA into Cellular Homeostasis

2013 ◽  
Vol 5 (5) ◽  
pp. a011080-a011080 ◽  
Author(s):  
R. Gilkerson ◽  
L. Bravo ◽  
I. Garcia ◽  
N. Gaytan ◽  
A. Herrera ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cellular Homeostasis ◽  
Mitochondrial Nucleoid

scholarly journals Mgm101p Is a Novel Component of the Mitochondrial Nucleoid That Binds DNA and Is Required for the Repair of Oxidatively Damaged Mitochondrial DNA

1999 ◽  
Vol 145 (2) ◽  
pp. 291-304 ◽  
Author(s):  
Shelly Meeusen ◽  
Quinton Tieu ◽  
Edith Wong ◽  
Eric Weiss ◽  
David Schieltz ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Subcellular Fractionation ◽  
Temperature Sensitive ◽  
Phenotypic Analysis ◽  
H2o2 Treatment ◽  
Mitochondrial Nucleoid ◽  
Mtdna Damage ◽  
Mtdna Replication ◽  
Essential Function ◽  
Nucleoid Structure

Maintenance of mitochondrial DNA (mtDNA) during cell division is required for progeny to be respiratory competent. Maintenance involves the replication, repair, assembly, segregation, and partitioning of the mitochondrial nucleoid. MGM101 has been identified as a gene essential for mtDNA maintenance in S. cerevisiae, but its role is unknown. Using liquid chromatography coupled with tandem mass spectrometry, we identified Mgm101p as a component of highly enriched nucleoids, suggesting that it plays a nucleoid-specific role in maintenance. Subcellular fractionation, indirect immunofluorescence and GFP tagging show that Mgm101p is exclusively associated with the mitochondrial nucleoid structure in cells. Furthermore, DNA affinity chromatography of nucleoid extracts indicates that Mgm101p binds to DNA, suggesting that its nucleoid localization is in part due to this activity. Phenotypic analysis of cells containing a temperature sensitive mgm101 allele suggests that Mgm101p is not involved in mtDNA packaging, segregation, partitioning or required for ongoing mtDNA replication. We examined Mgm101p's role in mtDNA repair. As compared with wild-type cells, mgm101 cells were more sensitive to mtDNA damage induced by UV irradiation and were hypersensitive to mtDNA damage induced by gamma rays and H2O2 treatment. Thus, we propose that Mgm101p performs an essential function in the repair of oxidatively damaged mtDNA that is required for the maintenance of the mitochondrial genome.

Mitochondrial Nucleoid Clustering Is Regulated by H2O2 Production in the Respiratory Chain

2012 ◽  
Vol 53 ◽  
pp. S52-S53
Author(s):  
Lukas Alan ◽  
Jaroslav Zelenka ◽  
Tomas Spacek ◽  
Petr Jezek
Keyword(s):  
Respiratory Chain ◽  
H2o2 Production ◽  
Mitochondrial Nucleoid

scholarly journals Loss of the Mitochondrial Nucleoid Protein, Abf2p, Destabilizes Repetitive DNA in the Yeast Mitochondrial Genome

Genetics ◽  
2008 ◽  
Vol 181 (1) ◽  
pp. 331-334 ◽  
Author(s):  
Rey A. Sia ◽  
Stephanie Carrol ◽  
Lidza Kalifa ◽  
Christine Hochmuth ◽  
Elaine A. Sia
Keyword(s):  
Mitochondrial Genome ◽  
Repetitive Dna ◽  
Mitochondrial Nucleoid
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