Validated HPLC Method for Simultaneous Determination of Aripiprazole and CoAdministered Clonazepam in Spiked Human Plasma

2017 ◽  
Vol 3 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Christine M. El-Maraghy ◽  
Hesham Salem ◽  
Sawsan M. Amer ◽  
Marianne Nebsen
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Hplc Method ◽  
Spiked Human Plasma

Validated HPLC Method for the simultaneous determination of acyclovir and co-administered vitamin B3 and gabapentin in spiked human plasma

2018 ◽  
Vol 1 (7) ◽  
pp. 475-482 ◽  
Author(s):  
Rania Sayed ◽  
Manal El-Masry ◽  
Wafaa Hassan ◽  
Magda El-Mammli ◽  
Abdalla Shalaby ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Hplc Method ◽  
Vitamin B3 ◽  
Spiked Human Plasma

scholarly journals Development and Validation of HPLC Method for Simultaneous Determination of Ceftriaxone and Cefaclor in Commercial Formulations and Biological Samples

2017 ◽  
Vol 57 (4) ◽  
Author(s):  
Jasmin Shah ◽  
M. Rasul Jan ◽  
Sultan Shah ◽  
M. Naeem Khan
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
High Performance ◽  
Limit Of Detection ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Limit Of Quantification ◽  
Spiked Human Plasma

A reversed phase high performance liquid chromatographic method has been developed for the simultaneous determination of cefaclor and ceftriaxone cephalosporin antibiotic. The developed method has been validated and applied to mixtures of the commercial formulation and spiked human plasma. A mediterranea C<sub>18</sub> column (4.6 × 250 mm) was used with isocratic solvent delivery system and UV-visible detector. Different experimental parameters like solvent composition (acetonitrile: methanol: triethyl amine buffer 1:1:2 (v/v), flow rate of mobile phase (0.6 mLmin<sup>-1</sup>), pH of the buffer (7), and wavelength (260 nm) were optimized for effective separation and esolution of the analyte peaks. The separation was achieved in 6 min with retention times of 4.94 ± 0.056 min and 3.39 ± 0.022 min for cefaclor and ceftriaxone respectively. The linear range for both the studied drugs was found to be 0.5-250 μgmL<sup>-1</sup> with r<sup>2</sup> of 0.9987 (cefaclor) and 0.9997 (ceftriaxone). The limit of detection (3.3 σ/S) was found to be 2.34 × 10<sup>-2</sup> μgmL−1 and 1.70 × 10−2 μgmL<sup>-1</sup>, respectively, for cefaclor and ceftriaxone. Similarly limit of quantification (10σ/S) was 7.10 × 10−2 μgmL<sup>-1</sup> for cefaclor and 5.15 × 10−2 μgmL<sup>-1</sup> for ceftriaxone. The chromatographic procedure was applied to commercial formulations and spiked human plasma and the results were compared with literature HPLC method.

scholarly journals High Performance Liquid Chromatoqraphic Method for the Simultaneous Determination of Labetalol and Hydrochlorothiazide in Tablets and Spiked Human Plasma

2004 ◽  
Vol 72 (2) ◽  
pp. 143-155 ◽  
Author(s):  
M. Sultan ◽  
H. Abdine ◽  
N. Zoman ◽  
F. Belal
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
High Performance ◽  
Internal Standard ◽  
Reversed Phase ◽  
Hplc Method ◽  
Spiked Human Plasma ◽  
Quantitation Limit

A reversed-phase HPLC method with spectrophotometric detection was developed for the simultaneous determination of labetalol (LBT) and hydrochloro-thiazide (HCD). The chromatographic separation was performed using a Microbondapak C18 column (4.6 i.d. x 250 nm) and paracetamol as internal standard. A mobile phase consisting of 0.05 M phosphate buffer/acetonitrile of pH 4 (7:3) at a flow rate of 0.7 ml/min was used. The detection was affected spectrophotornetrically at 302 nm. The working concentration range was 0.3–10 µg/ml with detection limits of 0.05 µg/ml for both drugs. The lower quantitation limit was 0.25 µg/ml in the two cases. The method was successfully applied to tablets, the % recoveries were 99.45 ± 0.68 for LBT and 99.79 ± 0.75 for HCD. The method was extended to the in-vitro determination in spiked human plasma. The % recoveries were 91.12 ± 0.33 for LBT and 91.37 ± 0.40 for HCD. The interday and intraday precision and accuracy were evaluated in plasma by calculating the % RSD (n=5) and the % error and were found to be in the ranges of 1.18–4.1% and 0.38–0.36% for both drugs, respectively.

An HPLC method for the simultaneous determination of neurotoxic dipyridyl isomers in human plasma

2007 ◽  
Vol 45 (1) ◽  
pp. 120-124
Author(s):  
Ahmad H. Malkawi ◽  
Abeer M. Al-Ghananeem ◽  
Shama Nasim ◽  
Jose de Leon ◽  
Peter A. Crooks
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Hplc Method

Development and validation of a rapid HPLC method for simultaneous determination of tramadol, and its two main metabolites in human plasma

2006 ◽  
Vol 830 (2) ◽  
pp. 207-211 ◽  
Author(s):  
Mohammad-Reza Rouini ◽  
Yalda Hosseinzadeh Ardakani ◽  
Faezeh Soltani ◽  
Hassan Y. Aboul-Enein ◽  
Alireza Foroumadi
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Hplc Method ◽  
Development And Validation
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