Association of cumulus cell gene expression with embryological indicators in assisted reproductive technology programs

2016 ◽  
Vol 7_2016 ◽  
pp. 60-66
Author(s):  
Safronova N.A. Safronova ◽  
Kalinina E.A. Kalinina ◽  
Donnikov A.E. Donnikov ◽  
Burmenskaya O.V. Burmenskaya ◽  
Makarova N.P. Makarova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Assisted Reproductive Technology ◽  
Cumulus Cell ◽  
Reproductive Technology ◽  
Cell Gene Expression ◽  
Technology Programs ◽  
Cell Gene

Cumulus cell gene expression in luteal-phase-derived oocytes after double stimulation in one menstrual cycle

2020 ◽  
Vol 41 (3) ◽  
pp. 518-526
Author(s):  
Nona Mishieva ◽  
Bella Martazanova ◽  
Khava Bogatyreva ◽  
Anna Korolkova ◽  
Anastasia Kirillova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Menstrual Cycle ◽  
Luteal Phase ◽  
Cumulus Cell ◽  
Cell Gene Expression ◽  
Cell Gene ◽  
Double Stimulation

Differential cumulus cell gene expression in young women with diminished ovarian reserve

2007 ◽  
Vol 88 ◽  
pp. S66
Author(s):  
K. Greenseid ◽  
S. Jindal ◽  
M. Nihsen ◽  
J.M. Hurwitz ◽  
N.S. Santoro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Young Women ◽  
Ovarian Reserve ◽  
Cumulus Cell ◽  
Diminished Ovarian Reserve ◽  
Cell Gene Expression ◽  
Cell Gene

Association between early embryo morphokinetics plus cumulus cell gene expression and assisted reproduction outcomes in polycystic ovary syndrome women

2019 ◽  
Vol 38 (2) ◽  
pp. 139-151 ◽  
Author(s):  
Nasim Tabibnejad ◽  
Mohammad Hasan Sheikhha ◽  
Nasrin Ghasemi ◽  
Farzaneh Fesahat ◽  
Mehrdad Soleimani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polycystic Ovary Syndrome ◽  
Assisted Reproduction ◽  
Polycystic Ovary ◽  
Cumulus Cell ◽  
Early Embryo ◽  
Ovary Syndrome ◽  
Cell Gene Expression ◽  
Cell Gene

scholarly journals Erratum to: Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects

2014 ◽  
Vol 31 (8) ◽  
pp. 1111-1111
Author(s):  
Timur Gurgan ◽  
Debbie Montjean ◽  
Aygul Demirol ◽  
Moncef Benkhalifa ◽  
Yves J. R. Menezo
Keyword(s):  
Gene Expression ◽  
Cumulus Cell ◽  
Cell Gene Expression ◽  
Cell Gene

262. Human cumulus cell gene expression as a marker of clinical embryo grade

2008 ◽  
Vol 20 (9) ◽  
pp. 62
Author(s):  
K. M. Gebhardt ◽  
D. Feil ◽  
M. Lane ◽  
D. L. Russell
Keyword(s):  
Gene Expression ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Embryo Morphology ◽  
Low Grade ◽  
Necrosis Factor Alpha ◽  
Developmental Potential ◽  
Quality Grade ◽  
Cell Gene Expression ◽  
Cell Gene

In Australia, Assisted Reproductive Technology (ART) accounts for ~3% of births. However, the success rate remains around 65% for women under 35 years of age, hence multiple embryo transfer is frequently preferred to improve the probabiity of achieving a term pregnancy. A biochemical marker for oocyte and embryo developmental potential would augment successful pregnancy outcomes following IVF/ICSI by optimising oocyte and embryo selection, therefore increasing the number of single embryo transfers (SET) performed in ART cycles. Changes in expression levels in human cumulus cells may reflect the quality of their enclosed oocyte. We investigated cumulus cell gene expression and subsequent embryo development to find a marker of embryo quality. Paired samples of cumulus cells were collected from oocytes that progressed to embryos of either high or low grade from eleven IVF/ICSI patients. Following cumulus oocyte complex retrieval cumulus cells were trimmed from the oocyte, and all oocytes and resulting embryos were cultured and tracked individually. Cumulus cell gene expression was assessed using a real-time RT–PCR assay, measuring expression of cyclooxygenase 2 (COX2; PTGS2), Pentraxin 3 (PTX3), Versican (VCAN), Tumour Necrosis Factor Alpha Induced protein 6 (TNAIFP6; TSG6), Lactate Dehydrogenase A (LDHA), Phosphofructokinase Platelet (PFKP), Gremlin (GREM1), Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and 18S rRNA. Standard curves using plasmid subclones for each target were run to assess copy numbers of genes. Embryo morphology was assessed by an embryologist and correlated with relative gene expression. Cumulus cell gene expression was altered in cumulus cells from oocytes which subsequently developed into higher quality (Grade 1 and 2) embryos compared with cumulus cells from oocytes which developed into lower quality (Grade 3 and 4) embryos. This may lead to establishment of markers prognostic for developmental outcome, facillitating more reliable selection of higher quality embryos, increasing single embryo transfers and improving health outcomes from ART.

scholarly journals Cumulus cell gene expression predicts better cleavage-stage embryo or blastocyst development and pregnancy for ICSI patients

2011 ◽  
Vol 26 (5) ◽  
pp. 1035-1051 ◽  
Author(s):  
S. Wathlet ◽  
T. Adriaenssens ◽  
I. Segers ◽  
G. Verheyen ◽  
H. Van de Velde ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cumulus Cell ◽  
Cleavage Stage ◽  
Blastocyst Development ◽  
Cleavage Stage Embryo ◽  
Stage Embryo ◽  
Cell Gene Expression ◽  
Cell Gene
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