scholarly journals Identification of the Gram Positive Bacterial Sepsis Agent with Rapid Genotype Test

2021 ◽  
Author(s):  
Ziya ERDOĞAN ◽  
Cihadiye ÖZTÜRK ◽  
Şengül CANGÜR ◽  
Emel ÇALIŞKAN ◽  
Şükrü ÖKSÜZ ◽  
...  
Keyword(s):  
Bacterial Sepsis ◽  
Gram Positive ◽  
Genotype Test

scholarly journals Difference of Procalcitonin Levels in Gram-Positive and Gram-Negative Bacterial Sepsis Patients of Indonesia Army Central Hospital Gatot Soebroto in 2016

2022 ◽  
Vol 13 (1) ◽  
pp. 38
Author(s):  
Nindy Handayani ◽  
Soroy Lardo ◽  
Nunuk Nugrohowati
Keyword(s):  
Mean Difference ◽  
P Value ◽  
Central Hospital ◽  
Bacterial Sepsis ◽  
Gram Positive ◽  
Cross Sectional ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Significant Difference ◽  
The Mean

Introduction: Procalcitonin is known as a marker of infection and indicator for severity of infections. In sepsis, elevated procalcitonin levels in blood have a significant value that can be used as a sepsis biomarker. The aim of this study was to determine the mean difference of procalcitonin levels in Gram-positive and Gram-negative bacterial sepsis patients.Methods: This study used quantitative method with cross sectional approach. The sample of this study were bacterial sepsis patients of Indonesia Army Central Hospital Gatot Soebroto in 2016 which were divided into two groups: Gram-positive and Gram-negative bacterial sepsis patients with the number of each group was 30 samples. The data were analyzed by using independent t test.Results: This study showed that mean levels of procalcitonin in Gram-positive bacterial sepsis patients was 6.47 ng/ml and Gram-negative was 66.04 ng/ml. There was a significant difference between mean levels of procalcitonin in Gram-positive and Gram-negative bacterial sepsis patients of Indonesia Army Central Hospital Gatot Soebroto in 2016 with p value = 0.000 (p < 0.05).Conclusion: The mean difference of procalcitonin levels in Gram-negative bacterial sepsis patients were higher than Gram-positive bacterial sepsis patients, because Gram-negative bacteria have lipopolysaccharide which is a strong immunostimulator and increases TNF-α production higher than Gram-positive bacteria. 

Peptidoglycan and Lipoteichoic Acid in Gram-Positive Bacterial Sepsis: Receptors, Signal Transduction, Biological Effects, and Synergism

Shock ◽  
2003 ◽  
Vol 20 (5) ◽  
pp. 402-414 ◽  
Author(s):  
Jacob E. Wang ◽  
Maria K. Dahle ◽  
Michelle McDonald ◽  
Simon J. Foster ◽  
Ansgar O. Aasen ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Biological Effects ◽  
Lipoteichoic Acid ◽  
Bacterial Sepsis ◽  
Gram Positive

Differential gene sets profiling in gram-negative and gram-positive sepsis

2021 ◽  
Author(s):  
Qingliang Wang ◽  
Xiaojie Li ◽  
Wenting Tang ◽  
Xiaoling Guan ◽  
Zhiyong Xiong ◽  
...  
Keyword(s):  
Host Response ◽  
Gene Set Enrichment Analysis ◽  
Validation Dataset ◽  
Bacterial Sepsis ◽  
Gram Positive ◽  
Gram Negative ◽  
Gene Set ◽  
Gene Sets ◽  
Differential Gene ◽  
The Difference

Abstract Introduction: The host response to bacterial sepsis is reported to be nonspecific regardless of the causative pathogen. However, newer paradigms indicated that host response of Gram-negative sepsis may be different from Gram-positive sepsis and the difference has not been clearly clarified. The current study aimed to explore the difference by identifying the differential gene sets using genome-wide technique.Methods: The training dataset GSE6535 and the validation dataset GSE13015 were used for bioinformatics analysis. The distinct gene sets of sepsis with different infections were screened using Gene set variation analysis (GSVA) and Gene set enrichment analysis (GSEA). The intersection gene sets based on the two algorithms were confirmed through Venn analysis. Finally, the common gene sets between GSE6535 and GSE13015 were determined by GSVA.Results: Two immunologic gene sets in GSE6535 were identified based on GSVA, which could be used to discriminate sepsis caused by Gram-positive, Gram-negative or mixed infection. A total of 19 gene sets were obtained in GSE6535 through Venn analysis, revealed the heterogeneity of sepsis between gram-negative bacteria and gram-positive bacteria at the molecular level. The result was also verified by analysis the validation set GSE13015, 31 gene sets were identified by GSVA and GSEA. Furthermore, 10 common differential gene sets were finally confirmed based on GSVA for dataset GSE6535 and GSE13015.Conclusions: Our data indicated that host response may differ dramatically depending on the inciting organism. The findings offer new insight to investigate the pathophysiology of bacterial sepsis.

scholarly journals Study of Platelet Parameters with Different Isolated Organism in Neonatal Bacterial Sepsis

2021 ◽  
pp. 14-20
Author(s):  
Rajiv Kumar Prasad ◽  
Amita Uday Surana ◽  
Chetan Chovatiya ◽  
Radhika Iyer ◽  
Nidhi Modi
Keyword(s):  
Prospective Observational Study ◽  
Platelet Response ◽  
Bacterial Sepsis ◽  
Coagulase Negative Staphylococci ◽  
Gram Positive ◽  
Gram Negative ◽  
Significant Difference ◽  
Prolonged Duration ◽  
Severe Degree ◽  
Culture Positive

Background: Thrombocytopenia is a common hematological abnormality observed in neonatal sepsis and considered as early, nonspecific marker of sepsis. The studies related to organism specific platelet response are few. Objective: To assess the prevalence of thrombocytopenia and to study changes in various platelet parameters in relation to different isolated organism. Methods: A prospective observational study involving neonates with 1st episode of culture positive sepsis was done over a period of 18 months. The platelet parameters studied were incidence, degree, duration of thrombocytopenia; mean platelet volume and platelet nadir among neonate with specific organism isolated. Results: Out of 114 culture positive sepsis 31% Klebsiella, 30% Pseudomonas, 13% Citrobacter, 18% CONS, 4% Staphylococcus Aureus (S Aureus) and 3% had Acinetobacter sepsis. Overall prevalence of thrombocytopenia was 88%, of which klebsiella and S. Aureus sepsis observed 100% prevalence of thrombocytopenia followed by 88% pseudomonas, 80% coagulase negative staphylococci (CONS), 75% Acinetobacter and 66% Citrobacter sepsis. The proportion of severe degree of thrombocytopenia (18% Vs 4%), higher MPV (61% Vs 54%) and longer duration of thrombocytopenia (3.63 ± 0.49 Vs 2.95 ± 0.52) was observed more with Gram negative sepsis than with Gram positive sepsis and statistically significant difference in platelet nadir was observed with Gram negative sepsis. Severe degree of thrombocytopenia was seen in 50% neonates with Acinetobacter and 23% with Klebsiella sepsis. Acinetobacter, Klebsiella and Pseudomonas sepsis had higher mean MPV value, longer duration of thrombocytopenia and lowest platelet nadir.  The platelet parameters were less affected with Gram positive organism. Conclusion: Thrombocytopenia is a frequent occurrence in neonates with sepsis especially with Gram negative organism. Sepsis with Acinetobacter, Klebsiella & Pseudomonas organism was associated with prolonged duration, higher MPV and lower platelet count as compared to                    other isolated organisms.

Gram-positive bacterial sepsis in rat and tissue lipolytic activity on commercial parenteral fat emulsions

Infection ◽  
1990 ◽  
Vol 18 (2) ◽  
pp. 109-112 ◽  
Author(s):  
Z. Meraïhi ◽  
O. Lutz ◽  
A. Frey ◽  
A. C. Bach ◽  
J. M. Scheftel
Keyword(s):  
Lipolytic Activity ◽  
Bacterial Sepsis ◽  
Gram Positive ◽  
Fat Emulsions

scholarly journals Safety and efficacy of high-dose daptomycin as salvage therapy for severe gram-positive bacterial sepsis in hospitalized adult patients

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Chung-Chih Lai ◽  
Wang-Huei Sheng ◽  
Jann-Tay Wang ◽  
Aristine Cheng ◽  
Yu-Chung Chuang ◽  
...  
Keyword(s):  
Salvage Therapy ◽  
Adult Patients ◽  
High Dose ◽  
Bacterial Sepsis ◽  
Gram Positive ◽  
Safety And Efficacy

scholarly journals Serum procalcitonin levels distinguish Gram-negative bacterial sepsis from Gram-positive bacterial and fungal sepsis

2016 ◽  
Vol 21 (1) ◽  
pp. 39 ◽  
Author(s):  
Jiong Yang ◽  
Shuhua Li ◽  
Hengmo Rong ◽  
Qinliang Guo ◽  
Yifei Chen ◽  
...  
Keyword(s):  
Bacterial Sepsis ◽  
Gram Positive ◽  
Gram Negative ◽  
Fungal Sepsis

scholarly journals Treatment of Experimental Gram-Negative and Gram-Positive Bacterial Sepsis with the Hematoregulatory Peptide SK&F 107647

1996 ◽  
Vol 173 (1) ◽  
pp. 203-211 ◽  
Author(s):  
Peter L. DeMarsh ◽  
Grace I. Wells ◽  
Thomas F. Lewandowski ◽  
Carrie L. Frey ◽  
Pradip K. Bhatnagar ◽  
...  
Keyword(s):  
Bacterial Sepsis ◽  
Gram Positive ◽  
Gram Negative

scholarly journals Evaluation of Procalcitonin Accuracy for the Distinction Between Gram-Negative and Gram-Positive Bacterial Sepsis in Burn Patients

2018 ◽  
Vol 40 (1) ◽  
pp. 112-119 ◽  
Author(s):  
Luís Cabral ◽  
Vera Afreixo ◽  
Rita Meireles ◽  
Miguel Vaz ◽  
João-Gonçalo Frade ◽  
...  
Keyword(s):  
Burn Patients ◽  
Bacterial Sepsis ◽  
Gram Positive ◽  
Gram Negative

scholarly journals Glycomic analysis reveals a conserved response to bacterial sepsis induced by different bacterial pathogens.

2020 ◽  
Author(s):  
Daniel W. Heindel ◽  
Peter V. Aziz ◽  
Shuhui Chen ◽  
Jamey D. Marth ◽  
Lara K. Mahal
Keyword(s):  
Bacterial Pathogens ◽  
The Body ◽  
Strong Increase ◽  
Bacterial Sepsis ◽  
Gram Positive ◽  
Diagnostic Biomarkers ◽  
Lectin Blot ◽  
Weight Protein ◽  
Experimental Mouse

Sepsis is an extreme inflammatory response to infection (bacterial, viral, fungal) that occurs in the bloodstream and causes damage throughout the body. Currently, there are few diagnostic biomarkers of sepsis and new effective treatments have not been developed. There is a clear need to study the molecular underpinnings of this disease. Glycosylation is known to play a role in immunity and inflammation, but the role of glycans in sepsis is not well defined. Herein, we profiled the serum glycomes of experimental mouse sepsis models to identify changes induced by 4 different clinical bacterial pathogens (Gram-positive: Streptococcus pneumoniae, Staphylococcus aureus, Gram-negative: Escherichia coli and Salmonella Typhimurium) using our lectin microarray technology. We observed global shifts in the blood sera glycome that were conserved across all four species, regardless of whether they were Gram positive or negative. Bisecting GlcNAc was decreased upon sepsis and a strong increase in core 1/3 O-glycans was observed. Lectin blot analysis revealed a high molecular weight protein induced in sepsis by all four bacteria as the major cause of the core 1/3 O-glycan shift. While the identity of this protein remains to be elucidated, its presence indicates a common feature of bacterial sepsis associated with this glycomic signature.

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