scholarly journals Optimization of Surface Treatment on MEMS Probes for Single-cell Capture and Release

2019 ◽  
Vol 31 (9) ◽  
pp. 2873
Author(s):  
Kensaku Hayashi ◽  
Momoko Kumemura ◽  
Shohei Kaneda ◽  
Vivek Menon ◽  
Laurent Jalabert ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Single Cell ◽  
Cell Capture ◽  
Capture And Release

Single‐Domain Multiferroic Array‐Addressable Terfenol‐D (SMArT) Micromagnets for Programmable Single‐Cell Capture and Release

2021 ◽  
pp. 2006651
Author(s):  
Reem Khojah ◽  
Zhuyun Xiao ◽  
Mohanchandra K. Panduranga ◽  
Michael Bogumil ◽  
Yilian Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Domain ◽  
Cell Capture ◽  
Capture And Release

Single cell capture, isolation, and long‐term in‐situ imaging using quantitative self‐interference spectroscopy

2021 ◽  
Author(s):  
Rongxin Fu ◽  
Ya Su ◽  
Ruliang Wang ◽  
Xue Lin ◽  
Xiangyu Jin ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Capture ◽  
In Situ Imaging

scholarly journals A simple microdevice for single cell capture, array, release, and fast staining using oscillatory method

2018 ◽  
Vol 12 (3) ◽  
pp. 034105 ◽  
Author(s):  
Dantong Cheng ◽  
Yang Yu ◽  
Chao Han ◽  
Mengjia Cao ◽  
Guang Yang ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Capture

A Magnetic Dynamic Microbiointerface with Biofeedback Mechanism for Cancer Cell Capture and Release

2019 ◽  
Vol 11 (44) ◽  
pp. 41019-41029 ◽  
Author(s):  
Xiaohua Tian ◽  
Xiangyu Sha ◽  
Yonghai Feng ◽  
Yuqing Duan ◽  
Mingdong Dong ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cell Capture ◽  
Magnetic Dynamic ◽  
Capture And Release

scholarly journals Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

2019 ◽  
Vol 10 (5) ◽  
pp. 1506-1513 ◽  
Author(s):  
Min Li ◽  
Robbyn K. Anand
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Electrode Array ◽  
Cell Capture ◽  
Bipolar Electrodes ◽  
Manual Intervention ◽  
Electrical Lysis ◽  
Marker Free ◽  
Selection Of

We present integration of selective single-cell capture at an array of wireless electrodes (bipolar electrodes, BPEs) with transfer into chambers, reagent exchange, fluidic isolation and rapid electrical lysis in a single platform, thus minimizing sample loss and manual intervention steps.

Study on polyethylene glycol cross-linker in peptide-conjugated antibody on efficiency of cell capture and release

2020 ◽  
Vol 602 ◽  
pp. 113790
Author(s):  
Akifumi Yoshihara ◽  
Ryota Sekine ◽  
Yoshiyuki Yamada ◽  
Madoka Takai
Keyword(s):  
Polyethylene Glycol ◽  
Cell Capture ◽  
Capture And Release ◽  
Cross Linker

Abstract 2291: Single cell capture and molecular analysis of live CTCs using integrated microwells and single cell aspirator

2019 ◽  
Author(s):  
Charlotte N. Stahlfeld ◽  
Jake J. Tokar ◽  
David Quigley ◽  
David Niles ◽  
Jamie M. Sperger ◽  
...  
Keyword(s):  
Single Cell ◽  
Molecular Analysis ◽  
Cell Capture

Capillary Force Driven Single-Cell Spiking Apparatus for Studying Circulating Tumor Cells

2018 ◽  
Author(s):  
Jacob Amontree ◽  
Kangfu Chen ◽  
Jose Varillas ◽  
Z. Hugh Fan
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Single Cells ◽  
Lymphoblastic Leukemia ◽  
Surface Membrane ◽  
Critical Element ◽  
Cell Capture ◽  
Biomedical Sciences ◽  
Low Concentrations

The characterization of single cells within heterogeneous populations has great impact on both biomedical sciences and cancer research. By investigating cellular compositions on a broad scale, pertinent outliers may be lost in the sample set. Alternatively, an investigation focused on the behavior of specific cells, such as circulating tumor cells (CTCs), will reveal genetic biomarkers or phenotypic characteristics associated with cancer and metastasis. On average, CTC concentration in peripheral blood is extremely low, as few as one to two per billion of healthy blood cells. Consequently, the critical element lacking in many methods of CTC detection is accurate cell capture efficiency at low concentrations. To simulate CTC isolation, researchers usually spike small amounts of tumor cells to healthy blood for separation. However, spiking tumor cells at extremely low concentrations is challenging in a standard laboratory setting. We report our study on an innovative apparatus and method designed for low-cost, precise, and replicable single-cell spiking (SCS). Our SCS method operates solely from capillary aspiration without the reliance on external laboratory equipment. To ensure that our method does not affect the viability of each cell, we investigated the effects of surface membrane tensions induced by aspiration. Finally, we performed affinity-based CTC isolation using human acute lymphoblastic leukemia cells (CCRF-CEM) spiked into healthy whole blood with the SCS technique. The results of the isolation experiments demonstrate the reliability of our method in generating low-concentration cell samples.

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