scholarly journals Particle Patterning Based on Positive Dielectrophoresis Using a Scanning Microelectrode

2019 ◽  
Vol 31 (1) ◽  
pp. 23
Author(s):  
Tomoyuki Yasukawa ◽  
Takuma Gotoh ◽  
Takashi Yasuda ◽  
Masato Suzuki ◽  
Fumio Mizutani
Keyword(s):  
Positive Dielectrophoresis

Positive dielectrophoresis and heterogeneous aggregation in high-gradient ac electric fields

2002 ◽  
Vol 92 (5) ◽  
pp. 2829-2843 ◽  
Author(s):  
Zhiyong Qiu ◽  
Nikolai Markarian ◽  
Boris Khusid ◽  
Andreas Acrivos
Keyword(s):  
Electric Fields ◽  
High Gradient ◽  
Ac Electric Fields ◽  
Positive Dielectrophoresis

Array of Single-cell Pairs on a Microwell Array Based on Positive Dielectrophoresis

2014 ◽  
Vol 43 (7) ◽  
pp. 980-981 ◽  
Author(s):  
Yuki Yoshimura ◽  
Chiaki Fujii ◽  
Masahiro Tomita ◽  
Fumio Mizutani ◽  
Tomoyuki Yasukawa
Keyword(s):  
Single Cell ◽  
Microwell Array ◽  
Positive Dielectrophoresis

scholarly journals Bacterial handling under the influence of non-uniform electric fields: dielectrophoretic and electrohydrodynamic effects

2008 ◽  
Vol 80 (4) ◽  
pp. 627-638 ◽  
Author(s):  
Flavio H. Fernádez-Morales ◽  
Julio E. Duarte ◽  
Josep Samitier-Martí
Keyword(s):  
Electric Fields ◽  
Microelectrode Array ◽  
Viscous Drag ◽  
Liquid Motion ◽  
Dielectrophoretic Force ◽  
Electrode Surfaces ◽  
Biological Objects ◽  
Viscous Drag Force ◽  
Lift Off ◽  
Positive Dielectrophoresis

This paper describes the modeling and experimental verification of a castellated microelectrode array intended tohandle biocells, based on common dielectrophoresis. The proposed microsystem was developed employing platinumelectrodes deposited by lift-off, silicon micromachining, and photoresin patterning techniques. Having fabricated the microdevice it was tested employing Escherichia coli as bioparticle model. Positive dielectrophoresis could be verified with the selected cells for frequencies above 100 kHz, and electrohydrodynamic effects were observed as the dominant phenomena when working at lower frequencies. As a result, negative dielectrophoresis could not be observed because its occurrence overlaps with electrohydrodynamic effects; i.e. the viscous drag force acting on the particles is greater than the dielectrophoretic force at frequencies where negative dielectrophoresis should occur. The experiments illustrate the convenience of this kind of microdevices to micro handling biological objects, opening the possibility for using these microarrays with other bioparticles. Additionally, liquid motion as a result of electrohydrodynamic effects must be taken into account when designing bioparticle micromanipulators, and could be used as mechanism to clean the electrode surfaces, that is one of the most important problems related to this kind of devices.

Manipulation of Particle Motions and Their Segregation in Micro-Fluidics by Positive Dielectrophoresis

Materials ◽  
2003 ◽  
Author(s):  
Borus Khusid ◽  
Nikolai Markarian ◽  
Mike Yeksel ◽  
Kenneth R. Farmer ◽  
Andreas Acrivos
Keyword(s):  
Particle Model ◽  
Model Calculations ◽  
Channel Characteristics ◽  
Particle Accumulation ◽  
Micro Fluidics ◽  
Batch Fabrication ◽  
Dilute Suspensions ◽  
Ac Field ◽  
Positive Dielectrophoresis ◽  
Fitting Parameters

We study the ac-field-driven segregation of positively polarized particles flowing through a micro-channel. Our batch fabrication technique allows one to construct an apparatus featuring a large number of mechanically robust and chemically inert micro-devices having a very low ratio of the dielectrophoretic time to the fluid residence time. Experiments were performed on dilute suspensions in microfluidics with electrodes of different sizes arranged parallel and perpendicular to the flow. The application of a high-gradient strong ac field to a flowing suspension results in trapping particles in the channel. When the channel characteristics meet certain requirements, the predictions of a single-particle model for the particle accumulation along the channel are found to be consistent with the experimental data. The model calculations required no fitting parameters because the suspension properties were measured independently. The results of our studies validate a simulation model needed for the design and operation of dielectrophoretic micro-fluidics.

Separation and assisted patterning of hippocampal neurons from glial cells using positive dielectrophoresis

2015 ◽  
Vol 17 (3) ◽  
Author(s):  
Tianyi Zhou ◽  
Susan F. Perry ◽  
Yixuan Ming ◽  
Susanne Petryna ◽  
Vicki Fluck ◽  
...  
Keyword(s):  
Glial Cells ◽  
Hippocampal Neurons ◽  
Positive Dielectrophoresis

A combination of positive dielectrophoresis driven on-line enrichment and aptamer-fluorescent silica nanoparticle label for rapid and sensitive detection of Staphylococcus aureus

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4489-4497 ◽  
Author(s):  
Jingfang Shangguan ◽  
Yuhong Li ◽  
Dinggeng He ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silica Nanoparticle ◽  
Sensitive Detection ◽  
On Line ◽  
Positive Dielectrophoresis

The method combining positive dielectrophoresis (pDEP) enrichment and aptamer-fluorescent silica nanoparticle label forStaphylococcus aureusdetection is rapid and sensitive.

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