scholarly journals Changes in hematological and biochemical blood parameters in laboratory animals with experimental escherichiosis

2018 ◽  
Author(s):  
A. K. Shantyz ◽  
P. V. Miroshnichenko ◽  
E. S. Sadikova ◽  
V. V. Menshenin
Keyword(s):  
Blood Parameters ◽  
Laboratory Animals

The effect of the drug with hepatopro-tective activity "Hepaton" on the peripheral blood parameters of laboratory animals.

2020 ◽  
Vol 2 ◽  
pp. 116-121
Author(s):  
V. S. Ponomarev ◽  
Keyword(s):  
Peripheral Blood ◽  
Blood Clotting ◽  
White Blood Cells ◽  
Absolute Number ◽  
Blood Parameters ◽  
Relative Increase ◽  
Laboratory Animals ◽  
Control Groups ◽  
Chemical Agents ◽  
Observation Period

Today we can observe leak of hepatopro-tective agents, which increase the resistance of the liver to the damage by chemical agents and stabilize their metabolism in cases of tension detoxifying function, to-gether with a high need in them. The goal of this article was to find out the harmless-ness of the hepatoprotector “Hepaton” in laboratory conditions. For the experiment, were organized 8 groups, either for experiment and for con-trol, of rodents (rats), which include 10 animals each (both genders). Biochemical and hematological analyzes were ran out before the injection of the drug, 90 and 180 days after start of the experiment. Blood was obtained by puncture of the tail vein or (at the end of the study) after simultaneous guillotination. It should be mentioned that on the 90th and 180th day of the experiment, there was a mild, but statistically significant increase in the amount of hemoglobin, the absolute number of erythrocytes, a partial absolute and relative increase in count of white blood cells and platelets of peripheral blood. Blood clotting tests parameters for the whole observation period in experimental animals didn’t significantly differ from those at the start and in the control groups.

scholarly journals ДОСЛІДЖЕННЯ ГОСТРОЇ ТА ХРОНІЧНОЇ ТОКСИЧНОСТІ ЕКСПЕРИМЕНТАЛЬНОГО ПРЕПАРАТУ «МЕВЕСЕЛ-ІН’ЄКЦІЙНИЙ»

2016 ◽  
Vol 6 (2) ◽  
pp. 174-180
Author(s):  
B. Gutyj ◽  
M. Paska ◽  
N. Levkivska ◽  
R. Pelenyo ◽  
N. Nazaruk ◽  
...  
Keyword(s):  
Blood Parameters ◽  
Laboratory Animals ◽  
Average Dose ◽  
Toxic Substances ◽  
Internal Organs ◽  
Accumulation Coefficient ◽  
Short Time ◽  
Increased Activity ◽  
Toxicity Criteria ◽  
Total Average

<p>The results of study of toxicity of the newly created «Injectable Mevesel» during acute and chronic experiments are presented. There were no lethal cases at intragastric and intramuscular injections, only short-time inhibition of laboratory animals receiving the drug at a dose of 10.0 ml was observed. There were no lethal cases of test animals during the experiment in the conditions of study of accumulation properties of «Injectable Mevesel». Total average dose of the drug administered made up 162500 mg/kg, and accumulation coefficient was respectively 5.3.<br />In the study of morphological blood parameters of rats after intramuscular injection of «Injectable Mevesel» in increasing doses, probable increase in neutrophils count by 36.1%, and probable reduction in lymphocytes count by 15.2% were found.<br />Administration of the drug in increasing doses significantly affects the functional state of internal organs of experimental animals (liver) and causes significant degradation of the membranes of hepatocytes, as evidenced by increased activity of intracellular ALT, AST enzymes and alkaline phosphatase.<br />Therefore, new domestic drug «Injectable Mevesel» created by us belongs to class 4 toxicity criteria, i.e. low-toxic substances.</p>

scholarly journals Parameters of chronic toxicity of the solution for intrauterine use “Yodozol”

2019 ◽  
Vol 21 (95) ◽  
pp. 139-143
Author(s):  
R. M. Sachuk ◽  
Ya. S. Stravsky ◽  
O. A. Katsaraba ◽  
S. V. Zhigalyuk
Keyword(s):  
Caesarean Delivery ◽  
Chronic Toxicity ◽  
Study Drug ◽  
Blood Parameters ◽  
Preclinical Study ◽  
Laboratory Animals ◽  
Experimental Animals ◽  
Adverse Health Effects ◽  
Analgesic Effects ◽  
Light Yellow

The study of the chronic toxicity of the solution for intrauterine use in the form of aerosol – “Yodosol”, is a mandatory stage of preclinical study of the drug, which is a new development of PE «Biopharm» and the Experimental Station of epizootology of the Institute of Infectious Diseases of NAAN. In a scientific experiment on laboratory animals, it is possible to evaluate the safety of the drug at different times of use, to determine the area of toxic action and doses that do not cause adverse health effects. Therefore, the purpose of the study was to study the chronic toxicity of the drug “Yodosol”, in particular the determination of tolerant and toxic doses for rodents. The experiments were carried out in accordance with the requirements for biomedical research, the selection of analogues for testing and control, ensuring the same conditions of feeding and retention, as well as accounting for results. “Yodosol” is a light yellow foamy liquid with 1 ml containing 5 mg iodine and 10 mg potassium iodide. The drug is used for the prevention and treatment of postpartum intrauterine infections in cows, pigs, sheep and goats (endometritis, pyrometers, cervicitis, vaginitis, delayed litter caused by iodine-sensitive microorganisms), after delivery of rhododerma, caesarean delivery and caesarean delivery. The drug has antimicrobial, anti-inflammatory and analgesic effects, improves proliferative processes in the tissues of the genital organs, helps to reduce the time of recovery of animals. The drug is used according to the guideline, after its livestock products are used without restrictions. No significant deviations in the behavior of the experimental animals were observed in the studies of chronic toxicity of the drug “Yodosol”. Visually noted only a slight inhibition of the rats of the experimental group, which received a 10-fold overdose. According to biochemical studies, in the group, compared with the control animals, there was a slight deviation of the indicators of protein metabolism and activity of hepatospecific enzymes: growth of total protein, alkaline phosphatase and aspartateaminotransferase. However, such changes in the blood parameters of the experimental animals were short-lived and restorative, characterizing the study drug as low-toxic. All studies conducted to determine the parameters of chronic toxicity of the solution for intrauterine use of “Yodosol” included in the registration materials of the medicinal product.

scholarly journals Comparative effectiveness of means to reduce negative effects of lead, cadmium and nitrates

2020 ◽  
Vol 27 ◽  
pp. 00019
Author(s):  
Vasily Dorozhkin ◽  
Galina Pavlenko ◽  
Natalia Pavlova ◽  
Dmitry Drozdov
Keyword(s):  
Heavy Metals ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Feed Additives ◽  
The Body ◽  
Laboratory Animals ◽  
Negative Effects ◽  
Protective Efficiency ◽  
Livestock Products ◽  
Cadmium And Lead

According to the constant deterioration of the environmental situation, the issue of preserving health of agricultural animals and obtaining safe livestock products is acute. One of the most effective ways to reduce the accumulation and ecotoxicants impact is pharmacological drugs usage and feed additives with different mechanisms of action. A comparative assessment of various agents exposed to heavy metals and nitrates in laboratory animals is presented. It was found that using feed with an increased content of heavy metals and nitrates increased the level of ecotoxicants in the organs and tissues of animals. Solvimin selenium enabled to reduce the accumulation of cadmium and lead in organism (up to 45 %), restore the normal amount of hemoglobin and the summation threshold, animals’ weight, protein and carbohydrate metabolism. However, it did not restore the indicators of the liver and kidneys functional state. The use of laricarvit showed a fairly high protective efficiency in removing cadmium (25– 30 %) and reducing the nitrates accumulation in the blood and muscles, normalizing the body weight of animals, the number of white blood cells, lymphocytes and methemoglobin. Detox injections successfully reduced the content of nitric nitrogen in the blood, in the liver and, to some extent, in the kidneys, while complete normalization of blood parameters was not observed.

Nutrient pattern of unsaturated fatty acids and vitamin E increase resting metabolic rate of overweight and obese women

2020 ◽  
pp. 1-9
Author(s):  
Habib Yarizadeh ◽  
Leila Setayesh ◽  
Caroline Roberts ◽  
Mir Saeed Yekaninejad ◽  
Khadijeh Mirzaei
Keyword(s):  
Vitamin E ◽  
Metabolic Rate ◽  
Unsaturated Fatty Acids ◽  
Resting Metabolic Rate ◽  
Blood Parameters ◽  
Cross Sectional Study ◽  
Obese Women ◽  
Cross Sectional ◽  
Dietary Nutrients ◽  
Nutrient Patterns

Abstract. Objectives: Obesity plays an important role in the development of chronic diseases including cardiovascular disease and diabetes. A low resting metabolic rate (RMR) for a given body size and composition is a risk factor for obesity, however, there is limited evidence available regarding the association of nutrient patterns and RMR. The aim of this study was to determine the association of nutrient patterns and RMR in overweight and obese women. Study design: This cross-sectional study was conducted on 360 women who were overweight or obese. Method: Dietary intake was assessed using a semi-quantitative standard food frequency questionnaire (FFQ). Nutrient patterns were also extracted by principal components analysis (PCA). All participants were evaluated for their body composition, RMR, and blood parameters. Result: Three nutrient patterns explaining 64% of the variance in dietary nutrients consumption were identified as B-complex-mineral, antioxidant, and unsaturated fatty acid and vitamin E (USFA-vit E) respectively. Participants were categorized into two groups based on the nutrient patterns. High scores of USFA-vit E pattern was significantly associated with the increase of RMR (β = 0.13, 95% CI = 0.79 to 68.16, p = 0.04). No significant associations were found among B-complex-mineral pattern (β = −0.00, 95% CI = −49.67 to 46.03, p = 0.94) and antioxidant pattern (β = 0.03, 95% CI −41.42 to 22.59, p = 0.56) with RMR. Conclusion: Our results suggested that the “USFA-vit E” pattern (such as PUFA, oleic, linoleic, vit.E, α-tocopherol and EPA) was associated with increased RMR.

Determination of acute toxicity of Tiacyclin solution for injection in laboratory animals

2019 ◽  
Vol 331 (8) ◽  
pp. 25-27
Author(s):  
N.Y. Morozov ◽  
◽  
S.I. Tchukina ◽  
E.I. Koveshnikova ◽  
◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Laboratory Animals

Anesthesia for rabbits submitted to experimental surgeries: Report of a series of eight anesthesias

2020 ◽  
pp. 151-158
Author(s):  
Rafael Antonio Caldart Bedin ◽  
Maisa Schultz ◽  
Antonio Bedin
Keyword(s):  
Muscle Relaxation ◽  
Anesthetic Management ◽  
Laboratory Animals ◽  
Average Weight ◽  
Anesthetic Induction ◽  
Anesthetic Agents ◽  
Reasonable Degree ◽  
Bioethical Debate ◽  
Precordial Stethoscope

Anesthesia for laboratory animals is a matter of biomedical concern and one of the most present dilemmas in the current bioethical debate. The use of anesthetic agents in experimental surgery aims at analgesia and restraining the animal, in order to achieve a reasonable degree of muscle relaxation and to produce sufficient analgesia. This practice requires the use of protocols for the administration of safe and efficient doses. Eight New Zealand rabbits were submitted to laparotomies demonstrating the surgical technique discipline of the local medical course. For pre-anesthetic medication, acepromazine 1 mg.kg-1 associated with ketamine 15 mg.kg-1 was used subcutaneously. Anesthesia was maintained with isoflurane and oxygen under a laryngeal mask in a Mapleson D anesthesia system and under spontaneous breathing. Hydration was performed with 10 ml.kg-1 saline every hour. A thermal mattress was used. Precordial stethoscope, pulse oximetry and clinical parameters were used for monitoring. For euthanasia, ketamine 10 mg.kg-1 associated with potassium chloride 19.1% 1 ml.kg-1 was used intravenously. The average weight of the rabbits was 2721.25 ± 275.01 grams and the duration of the anesthetic procedure was 120 ± 87 minutes. Discussion. In long-term anesthesia, such as laparotomies, the use of pre-anesthetic medication and then anesthetic induction by the combination of agents is recommended. However, anesthetic management requires monitoring to prevent insufficient or excessive doses from occurring.

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