scholarly journals Impact of polyphenol-rich green tea extracts on the protection of DOPC monolayer against damage caused by ozone induced lipid oxidation

2018 ◽  
Vol 65 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Elżbieta Rudolphi-Skórska ◽  
Barbara Dyba ◽  
Barbara Kreczmer ◽  
Maria Filek

Effectiveness of green tea (compared to two single polyphenols) in removing ozone derived reactive oxygen species acting on dioleoyl phosphatidylcholine monolayers was determined. The lipid oxidation was followed by changes of mechanical properties of the layer spreaded on the aqueous subphase containing various amounts of ozone in presence and absence of polyphenolics. It was shown that tea extract (containing 8.5x10-4 mg/cm3 polyphenols) is capable of inactivating 0.4 ppm ozone.The DPPH radical scavenging test set polyphenols in the order of increasing activity, consistent with their protective effect in relation to lipid oxidation, showing the highest efficacy of EGCG.

2020 ◽  
Author(s):  
Md. Jahirul Islam ◽  
Md Obyedul Kalam Azad ◽  
Byung Ryeol Ryu ◽  
Md Hafizur Rahman ◽  
Jung Dae Lim ◽  
...  

Abstract Hemp adaptability through physiological and biochemical changes was studied under 10 LED light spectra and natural light in a controlled aeroponic system. Light treatments were imposed on 25 days aged seedlings for 16 hours daily (300 µmol m-2s-1) for 20 days. Plant accumulated highest Cannabidiol (CBD) in R7:B2:G1 light treatment, with relatively higher photosynthetic rate and lower reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. Tetrahydrocannabinol (THC) also accumulated higher in white, R8:B2, and R7:B2:G1 light with less evidence of stress modulated substances. These results indicated that CBD and THC have no or little relation with light-mediated abiotic stress in hemp plants. On the contrary, Tetrahydrocannabinolic acid (THCA) was accumulated higher in R6:B2:G1:FR1 and R5:B2:W2:FR1 light treatment along with lower photosynthetic rate and higher reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. However, Cannabidiolic acid (CBDA) was accumulated higher in R6:B2:G1:FR1 light treatment with higher stress modulated substances and lower physiological traits. CBDA was also accumulated higher in R8:B2 and R7:B2:G1 light treatments with less evidence of stress modulated substances. Besides, Greenlight influenced in CBD and CBDA synthesis where FR and UV-A (along with green) play a positive and negative role in this process, respectively. These results indicate that the role of THCA as a stress marker is more decisive in hemp plant than other cannabinoids under attributed light-mediated stress.


2018 ◽  
Vol 6 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Sumaya Farooq ◽  
Amit Sehgal

Green tea is commercially available in three forms: loose leaf, bagged and powdered. The objective of this study was to compare the radical scavenging capacity of different forms of green tea like loose leaf (3), bagged (2) and powdered matcha (2) of various brands. The green tea forms were prepared at 95-100°C for 5 min., to mimic conditions usually used for tea preparations at home. The comparison of combined IC50 values of different green tea forms (loose leaf, bagged and matcha) showed no significant difference in their radical scavenging activity except bagged tea that exhibited slightly more DPPH radical scavenging potential as compared to matcha. Individually, the Bud white loose leaf demonstrated highest antioxidant activity followed by Laplant bag, Lipton bag, Laplant loose, Gourmet matcha, Wow matcha and Lipton loose. These findings revealed that on the basis of form, it may not be possible to generalize which form of tea whether loose leaf, bagged or matcha, is more effective in scavenging free radicals.


Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 710
Author(s):  
Md. Jahirul Islam ◽  
Byeong Ryeol Ryu ◽  
Md. Obyedul Kalam Azad ◽  
Md. Hafizur Rahman ◽  
Eun Ju Cheong ◽  
...  

Hemp adaptability through physiological and biochemical changes was studied under 10 LED light spectra and natural light in a controlled aeroponic system. Light treatments were imposed on 25 days aged seedlings for 16 h daily (300 µmol m−2 s−1) for 20 days. Plant accumulated highest Cannabidiol (CBD) in R7:B2:G1 light treatment, with relatively higher photosynthetic rate and lower reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. Tetrahydrocannabinol (THC) also accumulated at a higher level in white, R8:B2, and R7:B2:G1 light with less evidence of stress-modulated substances. These results indicated that CBD and THC have no or little relation with light-mediated abiotic stress in hemp plants. On the contrary, Tetrahydrocannabinolic acid (THCA) was accumulated higher in R6:B2:G1:FR1 and R5:B2:W2:FR1 light treatment along with lower photosynthetic rate and higher reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. However, Cannabidiolic acid (CBDA) was accumulated higher in R6:B2:G1:FR1 light treatment with higher stress-modulated substances and lower physiological traits. CBDA was also accumulated higher in R8:B2 and R7:B2:G1 light treatments with less evidence of stress-modulated substances. Besides, Greenlight influenced CBD and CBDA synthesis where FR and UV-A (along with green) play a positive and negative role in this process. Overall, the results indicated that the treatment R7:B2:G1 enhanced the medicinal cannabinoids most, and the role of THCA as a stress marker is more decisive in the hemp plant than in other cannabinoids under attributed light-mediated stress.


2019 ◽  
Vol 3 (2) ◽  
pp. 66-72
Author(s):  

: The aim of the present study is to determine the total phenolic contents (TPC), DPPH radical scavenging activities, and reducing power of commercially consumed herbal teas including sage tea (ST), blackberry tea (BT), apple tea (AT), Echinacea tea (ET), lime tea (LT), rosehip tea (RT), and green tea (GT) in Turkey. For this purpose, various solvents were used for extraction of the tea samples and the results were compared in terms of total phenolic contents and antioxidant activities, as well. Hot water is found as the best solvent for the extraction of total phenolics from ST, BT, AT, and RT, respectively. On the other hand, the highest TPC values are obtained with the extraction of ET, LT, and GT in the presence of aqueous acetone (50%) solvent system. Generally all samples gave high DPPH radical scavenging activities depending on their extraction solvents. The highest radical scavenging activity was found as 94.92 ± 0.01 % for ST in ethanol, while the lowest activity was obtained as 5.98 ± 0.14 % for BT with water extract. Ferric reducing antioxidant assay results are closed each other and the highest activity (0.27 ± 0.74 mM Trolox) was observed in the ethanol extract of ST. Antioxidant activities and TPC of the herbal tea samples varied depending on the extraction solvent. Water or aqueous organic solvent extracts of sage tea and green tea samples gave high TPC values and antioxidant activities.


2018 ◽  
Vol 44 (4) ◽  
pp. 515-520
Author(s):  
M Kazemi

Carum copticum L. comprises several relevant species for food, cosmetic, perfumery and pharmaceutical industries. GC/MS analysis of the enential oil of C. copticum revealed γ- terpinene as a major component of C. copticum, with its contribution of 33.85%. Essential oils (EOs) exhibited a significant antimicrobial activity against all tested microbial strains. In addition, the C. copticum oil demonstrated the highest DPPH radical scavenging activity. These results clearly show the antimicrobial and antioxidant effects of the plant essential oil.


Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 105
Author(s):  
Sarah Fruehwirth ◽  
Sandra Egger ◽  
Dennis Kurzbach ◽  
Jakob Windisch ◽  
Franz Jirsa ◽  
...  

This study reports the impact of margarine-representative ingredients on its oxidative stability and green tea extract as a promising antioxidant in margarine. Oil-in-water emulsions received much attention regarding factors that influence their oxidative stability, however, water-in-oil emulsions have only been scarcely investigated. Margarine, a widely consumed water-in-oil emulsion, consists of 80–90% fat and is thermally treated when used for baking. As different types of margarine contain varying additives, their impact on the oxidative stability of margarine during processing is of pressing importance. Thus, the influence of different ingredients, such as emulsifiers, antioxidants, citric acid, β-carotene and NaCl on the oxidative stability of margarine, heated at 80 °C for 1 h to accelerate lipid oxidation, was analyzed by the peroxide value and oxidation induction time. We found that monoglycerides influenced lipid oxidation depending on their fatty acyl chain. α-Tocopheryl acetate promoted lipid oxidation, while rosemary and green tea extract led to the opposite. Whereas green tea extract alone showed the most prominent antioxidant effect, combinations of green tea extract with citric acid, β-carotene or NaCl increased lipid oxidation in margarine. Complementary, NMR data suggested that polyphenols in green tea extracts might decrease lipid mobility at the surface of the water droplets, which might lead to chelating of transition metals at the interface and decreasing lipid oxidation.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.


Sign in / Sign up

Export Citation Format

Share Document