scholarly journals Hydrogen sulfide generation from L-cysteine in the human glioblastoma-astrocytoma U-87 MG and neuroblastoma SHSY5Y cell lines

2017 ◽  
Vol 64 (1) ◽  
Author(s):  
Maria Wróbel ◽  
Patrycja Bronowicka-Adamska ◽  
Anna Bentke
Keyword(s):  
Hydrogen Sulfide ◽  
Cell Lines ◽  
Neuroblastoma Cells ◽  
Hplc Method ◽  
Human Glioblastoma ◽  
Astrocytoma Cells ◽  
Shsy5y Cell ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Catalyzed Reactions

Hydrogen sulfide (H2S) is endogenously synthesized from L-cysteine in reactions catalyzed by cystathionine beta-synthase (CBS, EC 4.2.1.22) and gamma-cystathionase (CSE, EC 4.4.1.1). The role of 3-mercaptopyruvate sulfurtransferase (MPST, EC 2.8.1.2) in H2S generation is also considered; it could be important for tissues with low CTH activity, e.g. cells of the nervous system. The expression and the activity of CBS, CTH, and MPST have been detected in the human glioblastoma-astrocytoma (U-87 MG) and neuroblastoma (SHSY5Y) cell lines. In both the cell lines, the expression and activity of MPST were the highest among the investigated enzymes, suggesting its possible role in the generation of H2S. The RP-HPLC method was used to determine cystathionine and alpha-ketobutyrate, products of the CBS and CTH-catalyzed reactions. A difference in cystathionine levels between cell homogenates with totally CTH-inhibiting concentrations of DL-propargylglycine and without the inhibitor was employed to evaluate the activity of CBS. A higher expression and the activities of the CBS, CTH, MPST in the neuroblastoma cells were associated with more an intensive generation of H2S in the presence of 2mM cysteine. A threefold higher level of sulfane sulfur, a potential source of hydrogen sulfide, was detected in the astrocytoma cells in comparison to the neuroblastoma cells.

scholarly journals The protective role of the 3-mercaptopyruvate sulfurtransferase (3-MST)-hydrogen sulfide (H2S) pathway against experimental osteoarthritis

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Sonia Nasi ◽  
Driss Ehirchiou ◽  
Athanasia Chatzianastasiou ◽  
Noriyuki Nagahara ◽  
Andreas Papapetropoulos ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Protective Role ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Experimental Osteoarthritis

scholarly journals Uterine Dysfunction in Diabetic Mice: The Role of Hydrogen Sulfide

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 917
Author(s):  
Emma Mitidieri ◽  
Domenico Vanacore ◽  
Carlotta Turnaturi ◽  
Raffaella Sorrentino ◽  
Roberta d’Emmanuele di Villa Bianca
Keyword(s):  
Hydrogen Sulfide ◽  
Cyclic Nucleotides ◽  
Nod Mice ◽  
Diabetic Mice ◽  
Uterine Contractions ◽  
Female Reproductive Health ◽  
Spontaneous Motility ◽  
Mercaptopyruvate Sulfurtransferase

It is well-known that the physiological uterine peristalsis, related to several phases of reproductive functions, plays a pivotal role in fertility and female reproductive health. Here, we have addressed the role of hydrogen sulfide (H2S) signaling in changes of uterine contractions driven by diabetes in non-obese diabetic (NOD) mice, a murine model of type-1 diabetes mellitus. The isolated uterus of NOD mice showed a significant reduction in spontaneous motility coupled to a generalized hypo-contractility to uterotonic agents. The levels of cyclic nucleotides, cAMP and cGMP, notoriously involved in the regulation of uterus homeostasis, were significantly elevated in NOD mouse uteri. This increase was well-correlated with the higher levels of H2S, a non-specific endogenous inhibitor of phosphodiesterases. The exposure of isolated uterus to L-cysteine (L-Cys), but not to sodium hydrogen sulfide, the exogenous source of H2S, showed a weak tocolytic effect in the uterus of NOD mice. Western blot analysis revealed a reorganization of the enzymatic expression with an upregulation of 3-mercaptopyruvate-sulfurtransferase (3-MST) coupled to a reduction in both cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) expression. In conclusion, the increased levels of cyclic nucleotides dysregulate the uterus peristalsis and contractility in diabetic mice through an increase in basal H2S synthesis suggesting a role of 3-MST.

PO-19 On the role of protease-activated receptors-1 and -2 (PAR-1 and PAR-2) in human glioblastoma cell lines

2010 ◽  
Vol 125 ◽  
pp. S171
Author(s):  
A. Dutra-Oliveira ◽  
T.C. Carneiro-Lobo ◽  
I. Bakker-Abreu ◽  
J. Scharfstein J ◽  
R.Q. Monteiro ◽  
...  
Keyword(s):  
Cell Lines ◽  
Glioblastoma Cell ◽  
Protease Activated Receptors ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
Glioblastoma Cell Lines

scholarly journals Role of Hydrogen Sulfide and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of the Endoplasmic Reticulum Stress Response in Hepatocytes

Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1692
Author(s):  
Theodora Panagaki ◽  
Elisa B. Randi ◽  
Csaba Szabo
Keyword(s):  
Endoplasmic Reticulum ◽  
Hydrogen Sulfide ◽  
Liver Disease ◽  
Er Stress ◽  
Chronic Liver Disease ◽  
Chronic Liver ◽  
Endoplasmic Reticulum Stress Response ◽  
Functional Responses ◽  
Mercaptopyruvate Sulfurtransferase

It is estimated that over 1.5 billion people suffer from various forms of chronic liver disease worldwide. The emerging prevalence of metabolic syndromes and alcohol misuse, along with the lack of disease-modifying agents for the therapy of many severe liver conditions predicts that chronic liver disease will continue to be a major problem in the future. Better understanding of the underlying pathogenetic mechanisms and identification of potential therapeutic targets remains a priority. Herein, we explored the potential role of the 3-mercaptopyruvate sulfurtransferase/hydrogen sulfide (H2S) system in the regulation of the endoplasmic reticulum (ER) stress and of its downstream processes in the immortalized hepatic cell line HepG2 in vitro. ER stress suppressed endogenous H2S levels and pharmacological supplementation of H2S with sodium hydrogen sulfide (NaHS) mitigated many aspects of ER stress, culminating in improved cellular bioenergetics and prevention of autophagic arrest, thereby switching cells’ fate towards survival. Genetic silencing of 3-MST or pharmacological inhibition of the key enzymes involved in hepatocyte H2S biosynthesis exacerbated many readouts related to ER-stress or its downstream functional responses. Our findings implicate the 3-MST/H2S system in the intracellular network that governs proteostasis and ER-stress adaptability in hepatocytes and reinforce the therapeutic potential of pharmacological H2S supplementation.

scholarly journals Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 237
Author(s):  
Armance Gelaude ◽  
Sylvain Slaby ◽  
Katia Cailliau ◽  
Matthieu Marin ◽  
Arlette Lescuyer-Rousseau ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Xenopus Laevis ◽  
Xenopus Laevis Oocytes ◽  
Oocyte Meiosis ◽  
M Phase ◽  
Dependent Inhibition ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Amplification Loop ◽  
Dose Dependent Inhibition

The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

scholarly journals Role of Hydrogen Sulfide in NRF2- and Sirtuin-Dependent Maintenance of Cellular Redox Balance

Antioxidants ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 129 ◽  
Author(s):  
Tiziana Corsello ◽  
Narayana Komaravelli ◽  
Antonella Casola
Keyword(s):  
Hydrogen Sulfide ◽  
Redox Homeostasis ◽  
Antioxidant Properties ◽  
Redox Balance ◽  
Cellular Redox ◽  
Endogenous Production ◽  
Organ Specific ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Antioxidant Effects

Hydrogen sulfide (H2S) has arisen as a critical gasotransmitter signaling molecule modulating cellular biological events related to health and diseases in heart, brain, liver, vascular systems and immune response. Three enzymes mediate the endogenous production of H2S: cystathione β-synthase (CBS), cystathione γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST). CBS and CSE localizations are organ-specific. 3-MST is a mitochondrial and cytosolic enzyme. The generation of H2S is firmly regulated by these enzymes under normal physiological conditions. Recent studies have highlighted the role of H2S in cellular redox homeostasis, as it displays significant antioxidant properties. H2S exerts antioxidant effects through several mechanisms, such as quenching reactive oxygen species (ROS) and reactive nitrogen species (RNS), by modulating cellular levels of glutathione (GSH) and thioredoxin (Trx-1) or increasing expression of antioxidant enzymes (AOE), by activating the transcription factor nuclear factor (erythroid-derived 2)-like 2 (NRF2). H2S also influences the activity of the histone deacetylase protein family of sirtuins, which plays an important role in inhibiting oxidative stress in cardiomyocytes and during the aging process by modulating AOE gene expression. This review focuses on the role of H2S in NRF2 and sirtuin signaling pathways as they are related to cellular redox homeostasis.

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