scholarly journals Age-dependent systemic DNA damage in early Type 2 Diabetes mellitus

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Dinko Rogulj ◽  
Ismail El Aklouk ◽  
Konjevoda Paško ◽  
Spomenka Ljubić ◽  
Mirjana Pibernik Okanović ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Oxidative Dna Damage ◽  
Ldl Cholesterol ◽  
Age Dependent

Oxidative stress, capable of eliciting damage to various biomolecules including DNA, is a recognized component of diabetes mellitus and its complications. Metabolic syndrome  (MetS) is associated with the development of type 2 diabetes mellitus  (T2DM), as well as other unfavorable outcomes. The aim of this study was to elucidate the role of oxidative stress in the development of T2DM, by investigating association of oxidative DNA damage with metabolic parameters in subjects with MetS and early T2DM.Selected anthropometric and biochemical parameters of MetS, inflammation and oxidative DNA damage: body mass index  (BMI), fatty liver index  (FLI), waist circumference  (WC), total cholesterol, HDL  and LDL-cholesterol, GGT, uric acid, total leukocyte/neutrophil count,  and urinary 8-oxo-deoxyguanosine  (u-8-oxodG) were assessed in male subjects with MetS and both younger  (≤55 years) and older  (>55 years) subjects with T2DM of short duration without complications.BMI, FLI, WC, total and LDL-cholesterol and  uric acid were higher, while the u-8-oxodG was lower in MetS group, when compared to older T2DM subjects. None of these parameters were different neither between MetS and younger T2DM, nor between two sub-groups of subjects with T2DM. Values of CRP, HDL-cholesterol, triglycerides, GGT, leukocytes and neutrophils were not different between all examined groups of subjects. Age-dependent increase in u-8-oxodG suggests that aging process, rather than metabolic disturbances or diabetes per se plays a pivotal role in development of oxidative DNA-damage in T2DM. Oxidative DNA damage cannot serve as an universal early marker of T2DM.

SIRT6 expression and oxidative DNA damage in individuals with prediabetes and type 2 diabetes mellitus

Gene ◽  
2018 ◽  
Vol 642 ◽  
pp. 542-548 ◽  
Author(s):  
Z. Çalışkan ◽  
T. Mutlu ◽  
M. Güven ◽  
M. Tunçdemir ◽  
M. Niyazioğlu ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Type 2 Diabetes Mellitus ◽  
Oxidative Dna Damage

scholarly journals Correlation of Uric Acid with Oxidative Stress and Endothelial Dysfunction in Type 2 Diabetes Mellitus

2021 ◽  
Author(s):  
Siva Prasad Palem
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Endothelial Dysfunction ◽  
P Value ◽  
Healthy Controls ◽  
Antioxidant Power

Objective: To evaluate the correlation of uric acid with oxidative stress and endothelial dysfunction in type 2 diabetic subjects.Material and Methods: The study included 120 subjects, among when 60 were type 2 diabetes subjects and the remaining 60 were age and gender matched healthy controls. The biochemical parameters, blood glucose, lipid profile, uric acid and homocysteine, were measured by standard kits in an autoanalyzer. Oxidative stress was evaluated by measuring malondialdehyde (MDA) and total antioxidant power by manual methods such as thio-barbituric acid reactive substances and ferric reducing ability of plasma (FRAP). Endothelial dysfunction was assessed by measuring nitric oxide (NO) by the kinetic cadmium method.Results: A significant elevation of triglycerides, low density lipoprotein (LDL), and MDA were observed in the type 2 diabetes mellitus patients while FRAP and NO were significantly reduced compared to the healthy controls. In addition, the uric acid levels had a highly significant correlation with FRAP (r=0.212, p-value=0.020), and moderately significant correlation with triglycerides (r=0.173, p-value=0.057) and homocysteine (r=0.178, p-value=0.051). Uric acid was negatively correlated with MDA and positively correlated with NO, but not statistically significant.Conclusion: Our findings suggest that uric acid may have antioxidant properties since it had a significant positive correlation with FRAP.

Antioxidant Activity of Uric Acid and its Correlation with Oxidative Stress and Endothelial Dysfunction in Type 2 Diabetes Mellitus

2020 ◽  
Author(s):  
P. Siva Prasad ◽  
P. Hari Prasad ◽  
T. Sudhakar ◽  
B. Rajkumar ◽  
Adithya Raj ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Antioxidant Activity ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Endothelial Dysfunction ◽  
Healthy Control ◽  
Type 2 Diabetic

Abstract Objective: Uric acid is an end product of purine metabolism and it has two different functions such as pro-oxidant and anti-oxidant. Where, pro-oxidant and anti-oxidants are opposite in action. Oxidative stress and endothelial dysfunction are a foremost cause of complications in diabetes mellitus, where uric acid may play a major role in this process. Hence, the present study has been designed to evaluate antioxidant activity of uric acid and its correlation with oxidative stress and endothelial dysfunction in type 2 diabetic subjects. Methods: We included 120 subjects in this study with age group of 39 -60 years. Among these 60 were type 2 diabetic subjects and 60 were healthy controls. The estimation of biochemical parameters such as blood sugar, lipid profile, uric acid, and homocysteine are measured in fully auto-analyzer with well recognized methods. MDA measured by TBARS method, total antioxidant capacity as FRAP and NO estimated by Kinetic cadmium method in spectrophotometer. Results: The study was found significant elevation of triglyceride, LDL and MDA and significant lower level of FRAP and NO in T2DM than healthy control. Uric acid was insignificant in T2DM compared to healthy control. However, uric acid has significant correlation with FRAP (r=0.2116, p=0.02) and moderate correlation with triglyceride (r=0.1736, p=0.0579) and homocysteine (r=0.1779, p=0.0519). MDA was negatively and NO was positively correlated with uric acid but statistically insignificant. Conclusion: We have found antioxidant activity of uric acid where it was determined by significant positive correlation with FRAP in type 2 diabetes mellitus.

scholarly journals CIRCULATING SERUM ADIPONECTIN AND OXIDATIVE STRESS BIOMARKERS IN PREDIABETES AND TYPE 2 DIABETES MELLITUS PATIENTS

2019 ◽  
pp. 58-60
Author(s):  
RACHNA SHARMA ◽  
SATYANARAYANA P ◽  
PALLAVI ANAND ◽  
SHRAWAN KUMAR
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diabetic Patients ◽  
Oxidative Stress Biomarkers ◽  
And Oxidative Stress

Objectives: Adipocytokines and oxidative stress have been independently studied in various morbidities, but their interrelationship in mediating insulin resistance and type 2 diabetes mellitus is still unclear. Thus, the present study was aimed at evaluating malondialdehyde (MDA) levels in pre-diabetes and diabetics and correlating it with adiponectin levels. Methods: Fifty controls, 50 pre-diabetic, and 50 diabetic patients were enrolled. Plasma MDA, uric acid, and adiponectin were measured in the blood samples. Serum MDA adiponectin was analyzed by enzyme-linked immunosorbent assay and uric acid was analyzed by uricase method. Results: Pre-diabetic and diabetic patients had higher levels of MDA and uric acid, while the level of adiponectin was low compared to controls. Correlation of MDA was positive with uric acid but negative with adiponectin in diabetic patients. Likewise, adiponectin and uric acid were also correlated negatively. In pre-diabetes, adiponectin was significantly and negatively correlated with MDA and uric acid. Conclusions: Pre-diabetic and diabetic patients have increased oxidative stress, which is also linked with adipokine abnormalities. From this study, we observed that oxidative stress suppresses adiponectin production which is the protective adipokine in hyperglycemia. Thus, oxidative stress may serve as an indicator or target for in the control of hyperglycemic stress.

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