Localization and role of RAP55/LSM14 in HeLa cells: a new finding on the mitotic spindle assembly

2015 ◽  
Vol 62 (3) ◽  
pp. 613-619 ◽  
Author(s):  
Donia Mili ◽  
Dane Georgesse ◽  
Abderraouf Kenani
Keyword(s):  
Hela Cells ◽  
Mitotic Spindle ◽  
Spindle Assembly ◽  
New Finding ◽  
Mitotic Spindle Assembly

scholarly journals Centriole-independent mitotic spindle assembly relies on the PCNT–CDK5RAP2 pericentriolar matrix

2020 ◽  
Vol 219 (12) ◽  
Author(s):  
Sadanori Watanabe ◽  
Franz Meitinger ◽  
Andrew K. Shiau ◽  
Karen Oegema ◽  
Arshad Desai
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Hela Cells ◽  
Mitotic Spindle ◽  
Spindle Assembly ◽  
Cell Type ◽  
Matrix Assembly ◽  
Spindle Formation ◽  
Pericentriolar Material ◽  
Mitotic Spindle Assembly

Centrosomes, composed of centrioles that recruit a pericentriolar material (PCM) matrix assembled from PCNT and CDK5RAP2, catalyze mitotic spindle assembly. Here, we inhibit centriole formation and/or remove PCNT–CDK5RAP2 in RPE1 cells to address their relative contributions to spindle formation. While CDK5RAP2 and PCNT are normally dispensable for spindle formation, they become essential when centrioles are absent. Acentriolar spindle assembly is accompanied by the formation of foci containing PCNT and CDK5RAP2 via a microtubule and Polo-like kinase 1–dependent process. Foci formation and spindle assembly require PCNT-CDK5RAP2–dependent matrix assembly and the ability of CDK5RAP2 to recruit γ-tubulin complexes. Thus, the PCM matrix can self-organize independently of centrioles to generate microtubules for spindle assembly; conversely, an alternative centriole-anchored mechanism supports spindle assembly when the PCM matrix is absent. Extension to three cancer cell lines revealed similar results in HeLa cells, whereas DLD1 and U2OS cells could assemble spindles in the absence of centrioles and PCNT-CDK5RAP2, suggesting cell type variation in spindle assembly mechanisms.

scholarly journals Computer simulations predict that chromosome movements and rotations accelerate mitotic spindle assembly without compromising accuracy

2009 ◽  
Vol 106 (37) ◽  
pp. 15708-15713 ◽  
Author(s):  
Raja Paul ◽  
Roy Wollman ◽  
William T. Silkworth ◽  
Isaac K. Nardi ◽  
Daniela Cimini ◽  
...  
Keyword(s):  
Computer Simulations ◽  
Mitotic Spindle ◽  
Spindle Assembly ◽  
Spindle Poles ◽  
Colorectal Cancer Cells ◽  
Microtubule Growth ◽  
Mitotic Spindle Assembly ◽  
Chromosome Movements ◽  
Realistic Geometry

The mitotic spindle self-assembles in prometaphase by a combination of centrosomal pathway, in which dynamically unstable microtubules search in space until chromosomes are captured, and a chromosomal pathway, in which microtubules grow from chromosomes and focus to the spindle poles. Quantitative mechanistic understanding of how spindle assembly can be both fast and accurate is lacking. Specifically, it is unclear how, if at all, chromosome movements and combining the centrosomal and chromosomal pathways affect the assembly speed and accuracy. We used computer simulations and high-resolution microscopy to test plausible pathways of spindle assembly in realistic geometry. Our results suggest that an optimal combination of centrosomal and chromosomal pathways, spatially biased microtubule growth, and chromosome movements and rotations is needed to complete prometaphase in 10–20 min while keeping erroneous merotelic attachments down to a few percent. The simulations also provide kinetic constraints for alternative error correction mechanisms, shed light on the dual role of chromosome arm volume, and compare well with experimental data for bipolar and multipolar HT-29 colorectal cancer cells.

scholarly journals The role of localization in the operation of the mitotic spindle assembly checkpoint

Cell Cycle ◽  
2009 ◽  
Vol 8 (16) ◽  
pp. 2650-2660 ◽  
Author(s):  
Maiko Lohel ◽  
Bashar Ibrahim ◽  
Stephan Diekmann ◽  
Peter Dittrich
Keyword(s):  
Mitotic Spindle ◽  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Mitotic Spindle Assembly

scholarly journals Novel insights into the mechanisms of mitotic spindle assembly by NEK kinases

2016 ◽  
Vol 3 (3) ◽  
pp. e1062952 ◽  
Author(s):  
Suzanna L. Prosser ◽  
Laura O'Regan ◽  
Andrew M. Fry
Keyword(s):  
Mitotic Spindle ◽  
Spindle Assembly ◽  
Mitotic Spindle Assembly
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