scholarly journals Antigen-specific lymphocyte proliferation as a marker of immune response in guinea pigs with sustained Helicobacter pylori infection.

2014 ◽  
Vol 61 (2) ◽  
Author(s):  
Eliza Miszczyk ◽  
Maria Walencka ◽  
Karolina Rudnicka ◽  
Agnieszka Matusiak ◽  
Wiesława Rudnicka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Immune Responses ◽  
Lymph Nodes ◽  
Peripheral Blood ◽  
Guinea Pigs ◽  
Initial Moment ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Proliferation Of Lymphocytes ◽  
H Pylori

Helicobacter pylori (H. pylori) bacteria are human pathogens causing symptomatic gastritis, peptic ulcer or gastric cancer. Little is known about the kinetics of immune responses in H. pylori infected patients because the initial moment of infection has not been identified. Various animal models are used to investigate the immune processes related to H. pylori infection. In this study we checked whether H. pylori infection in guinea pigs, mimicking natural H. pylori infection in humans, resulted in the development of specific immune responses to H. pylori antigens by measuring the proliferation of lymphocytes localized in mesenteric lymph nodes, spleen and peripheral blood. The maturity of macrophages and cytokines, delivered by monocyte-macrophage lineage or lymphocytes, were considered as mediators, which might influence the lymphocyte blastogenic response. The obtained results showed the activation of T cells localized in mesenteric lymph nodes by H. pylori antigens in H. pylori infected guinea pigs four weeks postinfection. The blastogenic activity of lymphocytes was shaped by their interaction with antigen presenting cells, which were present in the cell cultures during the whole culture period. Moreover, the balance between cytokines derived from adherent leukocytes including interleukin 8--IL-8 as well as interferon gamma--IFN-γ, and transforming growth factor beta--TGF-β delivered by lymphocytes, was probably important for the successful proliferation of lymphocytes. The H. pylori specific lymphocytes were not propagated in peripheral blood and spleen of H. pylori infected animals. The modulation of immunocompetent cells by H. pylori antigens or their different distribution cannot be excluded.

Inability of human clinical strains of Helicobacter pylori to colonize the alimentary tract of germfree rodents

1990 ◽  
Vol 36 (4) ◽  
pp. 237-241 ◽  
Author(s):  
Margherita T. Cantorna ◽  
Edward Balish
Keyword(s):  
Helicobacter Pylori ◽  
Lymph Nodes ◽  
Alimentary Tract ◽  
Sprague Dawley ◽  
Mesenteric Lymph Nodes ◽  
Immunodeficient Mice ◽  
Mesenteric Lymph ◽  
H Pylori ◽  
Rats And Mice ◽  
Culture Negative

Several attempts were made to colonize the alimentary tract and infect germfree BALB/c mice and germfree Sprague-Dawley rats with two human isolates of Helicobacter pylori. The alimentary tracts of mice, sacrificed at intervals between 1 day and 20 weeks after oral challenge, were culture negative for H. pylori. The alimentary tract, kidney, liver, and mesenteric lymph nodes were culture negative for H. pylori 5 h after intravenous challenge. Growth of H. pylori was inhibited by homogenates of murine stomach, small intestine, liver, and mesenteric lymph nodes. Germfree rats and mice do not appear to be readily colonized or infected by human strains of H. pylori. Key words: Helicobacter pylori, germfree mice, congenitally immunodeficient mice.

scholarly journals Oral Infection with Signature-Tagged Listeria monocytogenes Reveals Organ-Specific Growth and Dissemination Routes in Guinea Pigs

2011 ◽  
Vol 80 (2) ◽  
pp. 720-732 ◽  
Author(s):  
Jody A. Melton-Witt ◽  
Susanne M. Rafelski ◽  
Daniel A. Portnoy ◽  
Anna I. Bakardjiev
Keyword(s):  
Listeria Monocytogenes ◽  
Lymph Nodes ◽  
Guinea Pigs ◽  
Mesenteric Lymph Nodes ◽  
Intestinal Villi ◽  
Content Type ◽  
Direct Pathway ◽  
Mesenteric Lymph ◽  
Organ Specific ◽  
Small Intestinal

ABSTRACTListeria monocytogenescauses a serious food-borne disease due to its ability to spread from the intestine to other organs, a process that is poorly understood. In this study we used 20 signature-tagged wild-type clones ofL. monocytogenesin guinea pigs in combination with extensive quantitative data analysis to gain insight into extraintestinal dissemination. We show thatL. monocytogenescolonized the liver in all asymptomatic animals. Spread to the liver occurred as early as 4 h after ingestion via a direct pathway from the intestine to the liver. This direct pathway contributed significantly to the bacterial load in the liver and was followed by a second wave of dissemination via the mesenteric lymph nodes (indirect pathway). Furthermore, bacteria were eliminated in the liver, whereas small intestinal villi provided a niche for bacterial replication, indicating organ-specific differences in net bacterial growth. Bacteria were shed back from intestinal villi into the small intestinal lumen and reinfected the Peyer's patches. Together, these results support a novel dissemination model whereL. monocytogenesreplicates in intestinal villi, is shed into the lumen, and reinfects intestinal immune cells that traffic to liver and mesenteric lymph nodes, a process that occurs even during asymptomatic colonization.

scholarly journals Oral SMEDDS promotes lymphatic transport and mesenteric lymph nodes target of chlorogenic acid for effective T-cell antitumor immunity

2021 ◽  
Vol 9 (7) ◽  
pp. e002753
Author(s):  
Jun Ye ◽  
Yue Gao ◽  
Ming Ji ◽  
Yanfang Yang ◽  
Zhaohui Wang ◽  
...  
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Lymph Nodes ◽  
Chlorogenic Acid ◽  
Targeted Delivery ◽  
Immune Memory ◽  
Lymphatic Transport ◽  
Mesenteric Lymph Nodes ◽  
Transport Pathway ◽  
Mesenteric Lymph

BackgroundMesenteric lymph nodes (MLNs) are critical draining lymph nodes of the immune system that accommodate more than half of the body’s lymphocytes, suggesting their potential value as a cancer immunotherapy target. Therefore, efficient delivery of immunomodulators to the MLNs holds great potential for activating immune responses and enhancing the efficacy of antitumor immunotherapy. Self-microemulsifying drug delivery systems (SMEDDS) have attracted increasing attention to improving oral bioavailability by taking advantage of the intestinal lymphatic transport pathway. Relatively little focus has been given to the lymphatic transport advantage of SMEDDS for efficient immunomodulators delivery to the MLNs. In the present study, we aimed to change the intestinal lymphatic transport paradigm from increasing bioavailability to delivering high concentrations of immunomodulators to the MLNs.MethodsChlorogenic acid (CHA)-encapsulated SMEDDS (CHA-SME) were developed for targeted delivery of CHA to the MLNs. The intestinal lymphatic transport, immunoregulatory effects on immune cells, and overall antitumor immune efficacy of CHA-SME were investigated through in vitro and in vivo experiments.ResultsCHA-SME enhanced drug permeation through intestinal epithelial cells and promoted drug accumulation within the MLNs via the lymphatic transport pathway. Furthermore, CHA-SME inhibited tumor growth in subcutaneous and orthotopic glioma models by promoting dendritic cell maturation, priming the naive T cells into effector T cells, and inhibiting the immunosuppressive component. Notably, CHA-SME induced a long-term immune memory effect for immunotherapy.ConclusionsThese findings indicate that CHA-SME have great potential to enhance the immunotherapeutic efficacy of CHA by activating antitumor immune responses.

Immune Responses in Mice after Immunization with Antigens from Different Stages of the Parasite Schistosoma mansoni

2010 ◽  
Vol 65 (3-4) ◽  
pp. 289-302 ◽  
Author(s):  
Hanaa M. Gaber ◽  
Amany S. Maghraby ◽  
Mohamed Bastawy Ahmed ◽  
Andreas Ruppel ◽  
Mahmoud M. Bahgat
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Lymph Nodes ◽  
Schistosoma Mansoni ◽  
B Lymphocytes ◽  
T And B Lymphocytes ◽  
Mesenteric Lymph Nodes ◽  
Antigen Preparation ◽  
Mesenteric Lymph ◽  
Egg Antigen

Mice responses to immunization with Schistosoma mansoni antigens were investigated. Priming with cercarial antigen preparation (CAP) induced significant (P < 0.05) IgM, IgG, IgG2a, IgG2b, and IgA increases, while booster caused a significant IgG1 increase. A soluble worm antigen preparation (SWAP) caused significant IgG elevation. Priming with soluble egg antigen (SEA) caused significant IgM and IgG2a increases, while booster induced significant IgM, IgG and IgA increases. CAP-immunized mice sera (IMS) recognized CAP peptides ranging from 23 - 78 kDa. SWAP-IMS recognized SWAP peptides ranging from 40 - 75 kDa. SEA-IMS recognized SEA peptides ranging from 33 - 101 kDa. The cross-reactive peptides among the 3 antigens were identified. CAP caused significant increases in mesenteric lymph nodes (MLNs) CD4,8+, B lymphocytes, CD8+ thymocytes, CD4+ T and B splenocytes. SWAP priming caused significant increases in MLNs CD4,8+ thymocytes and B splenocytes. SWAP booster caused significant increases in MLNs CD8+ T and B lymphocytes, CD4,8+ thymocytes and CD4+ T and B splenocytes. SEA caused significant increase in CD4+ T cells.

In situ demonstration of migration of dendritic cells released from rat small intestine to mesenteric lymph nodes

2001 ◽  
Vol 120 (5) ◽  
pp. A183-A183
Author(s):  
H KOBAYASHI ◽  
H NAGATA ◽  
S MIURA ◽  
T AZUMA ◽  
H SUZUKI ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Small Intestine ◽  
Lymph Nodes ◽  
Rat Small Intestine ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph
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