scholarly journals Bacterial putative metacaspase structure from Geobacter sulfureducens as a template for homology modeling of type II Triticum aestivum metacaspase (TaeMCAII).

2012 ◽  
Vol 59 (3) ◽  
Author(s):  
Malgorzata Z Dudkiewicz ◽  
Ewa Piszczek
Keyword(s):  
Triticum Aestivum ◽  
Cell Death ◽  
Reaction Mechanism ◽  
Programmed Cell Death ◽  
Substrate Specificity ◽  
Homology Modeling ◽  
Cysteine Proteases ◽  
Biochemical Data ◽  
Type Ii ◽  
Homology Models

Metacaspases, cysteine proteases belonging to the peptidase C14 family, are suspected of being involved in the programmed cell death of plants, although their sequences and substrate specificity differ from those of animal caspases. At present, the knowledge on the metacaspase reaction mechanism is based only on biochemical data and homology models constructed on caspase templates. Here we propose a novel template for metacaspase modeling and demonstrate important advantages in comparison to the conventionally used caspase templates. We also point out the connection between plant and bacterial metacaspases, underlining the prokaryotic roots of Programmed Cell Death (PCD).

scholarly journals Mannose inhibition of nitrate uptake by wheat roots

2011 ◽  
Vol 79 (2) ◽  
pp. 107-110
Author(s):  
Jin Zhi Li ◽  
William John Cram ◽  
Guang Yuan He
Keyword(s):  
Triticum Aestivum ◽  
Cell Death ◽  
Steady State ◽  
Programmed Cell Death ◽  
Nitrate Uptake ◽  
Atp Depletion ◽  
Invasive Technique ◽  
Wheat Roots ◽  
Control Value ◽  
Non Invasive

The effect of mannose on nitrate uptake was investigated by a new non-invasive technique. Under normal condition, nitrate uptake by wheat (<em>Triticum aestivum</em> L.) roots was about 1-7 µmol gfwt<sup>-1</sup> h<sup>-1</sup>. After the addition of 10 mM mannose, net nitrate influx by wheat roots started to decrease and reached a new steady state at -40 ±50% of the control value after 73 min. After mannose supplied for 2 h, its removal caused net nitrate influx to be recovered to an original rate. Therefore, the inhibition of mannose on nitrate uptake is not due to the onset of programmed cell death because it starts too rapidly and it is reversible, however, it is probably due to Pi and consequent ATP depletion.

scholarly journals Role of Ced-3/ICE-family proteases in staurosporine-induced programmed cell death.

1996 ◽  
Vol 133 (5) ◽  
pp. 1041-1051 ◽  
Author(s):  
M D Jacobsen ◽  
M Weil ◽  
M C Raff
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Mammalian Cell ◽  
Mammalian Cells ◽  
Apoptotic Cell ◽  
Interleukin 1 ◽  
Cell Types ◽  
Cysteine Proteases ◽  
A Cell ◽  
Bona Fide

In the accompanying paper by Weil et al. (1996) we show that staurosporine (STS), in the presence of cycloheximide (CHX) to inhibit protein synthesis, induces apoptotic cell death in a large variety of nucleated mammalian cell types, suggesting that all nucleated mammalian cells constitutively express all of the proteins required to undergo programmed cell death (PCD). The reliability of that conclusion depends on the evidence that STS-induced, and (STS + CHS)-induced, cell deaths are bona fide examples of PCD. There is rapidly accumulating evidence that some members of the Ced-3/Interleukin-1 beta converting enzyme (ICE) family of cysteine proteases are part of the basic machinery of PCD. Here we show that Z-Val-Ala-Asp-fluoromethylketone (zVAD-fmk), a cell-permeable, irreversible, tripeptide inhibitor of some of these proteases, suppresses STS-induced and (STS + CHX)-induced cell death in a wide variety of mammalian cell types, including anucleate cytoplasts, providing strong evidence that these are all bona fide examples of PCD. We show that the Ced-3/ICE family member CPP32 becomes activated in STS-induced PCD, and that Bcl-2 inhibits this activation. Most important, we show that, in some cells at least, one or more CPP32-family members, but not ICE itself, is required for STS-induced PCD. Finally, we show that zVAD-fmk suppresses PCD in the interdigital webs in developing mouse paws and blocks the removal of web tissue during digit development, suggesting that this inhibition will be a useful tool for investigating the roles of PCD in various developmental processes.

Evidence of ceased programmed cell death in metaphloem sieve elements in the developing caryopsis of Triticum aestivum L.

PROTOPLASMA ◽  
2008 ◽  
Vol 234 (1-4) ◽  
pp. 87-96 ◽  
Author(s):  
Likai Wang ◽  
Zhuqing Zhou ◽  
Xuefang Song ◽  
Jiwei Li ◽  
Xiangyi Deng ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Triticum Aestivum L ◽  
Sieve Elements

scholarly journals Caspases in Cell Death, Inflammation, and Pyroptosis

2020 ◽  
Vol 38 (1) ◽  
pp. 567-595 ◽  
Author(s):  
Sannula Kesavardhana ◽  
R.K. Subbarao Malireddi ◽  
Thirumala-Devi Kanneganti
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Immune Responses ◽  
Cross Talk ◽  
Cysteine Proteases ◽  
Proteolytic Processing ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
New Research ◽  
Multicellular Organisms

Caspases are a family of conserved cysteine proteases that play key roles in programmed cell death and inflammation. In multicellular organisms, caspases are activated via macromolecular signaling complexes that bring inactive procaspases together and promote their proximity-induced autoactivation and proteolytic processing. Activation of caspases ultimately results in programmed execution of cell death, and the nature of this cell death is determined by the specific caspases involved. Pioneering new research has unraveled distinct roles and cross talk of caspases in the regulation of programmed cell death, inflammation, and innate immune responses. In-depth understanding of these mechanisms is essential to foster the development of precise therapeutic targets to treat autoinflammatory disorders, infectious diseases, and cancer. This review focuses on mechanisms governing caspase activation and programmed cell death with special emphasis on the recent progress in caspase cross talk and caspase-driven gasdermin D–induced pyroptosis.

Programmed cell death in wheat (Triticum aestivum L.) endosperm cells is affected by drought stress

PROTOPLASMA ◽  
2018 ◽  
Vol 255 (4) ◽  
pp. 1039-1052 ◽  
Author(s):  
Chao Li ◽  
Cheng Li ◽  
Bingbing Wang ◽  
Runqi Zhang ◽  
Kaiyong Fu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Cell Death ◽  
Drought Stress ◽  
Programmed Cell Death ◽  
Triticum Aestivum L
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