scholarly journals Zinc-binding proteins from boar seminal plasma -- isolation, biochemical characteristics and influence on spermatozoa stored at 4°C.

2011 ◽  
Vol 58 (2) ◽  
Author(s):  
Marzena Mogielnicka-Brzozowska ◽  
Paweł Wysocki ◽  
Jerzy Strzeżek ◽  
Władysław Kordan
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Binding Proteins ◽  
Control Sample ◽  
Zinc Binding ◽  
Shielding Effect ◽  
Sperm Plasma Membrane ◽  
Native Electrophoresis ◽  
Seminal Plasma Proteins ◽  
Fast Flow

Affinity chromatography on Chelating Sepharose Fast Flow Gel-Zn(2+) was used for fractionation of boar seminal plasma proteins. Approximately 30% of total boar seminal plasma proteins showed affinity for zinc ions (ZnBP fraction). Native electrophoresis (PAGE) of ZnBP revealed six protein fractions which separated into 27 bands under denaturing conditions (SDS/PAGE). Two-dimensional electrophoresis (2D PAGE) showed 148 polypeptides with isoelectric points mostly in the basic and neutral pH range. The zinc-binding proteins comprise mainly 10-20 kDa polypeptides which are probably members of the spermadhesin family. ZnBP present in the incubation mixture of spermatozoa stored for 1 or 24 h at 4 °C allowed preservation of a higher percentage of cells exhibiting linear motility in comparison to a control sample stored in PBS. Presented results indicate that proteins binding Zn(2+) ions have a shielding effect on the sperm plasma membrane and acrosome of spermatozoa, protecting these structures against consequences of cold shock.

scholarly journals Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C

2014 ◽  
Vol 58 (1) ◽  
pp. 163-168 ◽  
Author(s):  
Marzena Mogielnicka-Brzozowska ◽  
Anna Dziekońska ◽  
Rafał Strzeżek ◽  
Michał Załęcki ◽  
Anna Majewska ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Binding Proteins ◽  
Membrane Damage ◽  
Membrane Integrity ◽  
Control Sample ◽  
Incubation Mixture ◽  
Zinc Binding ◽  
Surface Binding ◽  
Positive Effects ◽  
Straight Line

AbstractSperm surface binding sites for non-zinc-binding proteins (nZnBPs) and zinc-binding proteins (ZnBPs) were studied by the fluorescence technique with biotin-labelled proteins. The nZnBPs binding pattern was unspecific, no characteristic sites on plasmalemma were found. ZnBPs were attached mainly to the acrosomal region of sperm head and to the sperm flagellum. ZnBPs added to the incubation mixture of the canine spermatozoa allowed the preservation of higher values for total motility, progressive motility, curvilinear line velocity, straight line velocity, and beat cross frequency (P < 0.05), both at time 0 and after 1 h incubation at 5ºC. The addition of nZnBPs to the incubation mixture caused only weak positive effects when compared with control sample (PBS). A higher percentage of canine-ejaculated spermatozoa with intact membranes were observed when ejaculate was incubated with ZnBPs in comparison to control sample stored with PBS (P < 0.05) or nZnBPs (P < 0.05). Spermatozoa diluted with ZnBPs and nZnBPs exhibited a higher percentage of cells with active mitochondria when compared with control, both at time 0 and after 1 h; however, no statistical differences were observed. Our results emphasise the role of seminal plasma protein in securing the correct quantity and availability of zinc ions as a component regulating the motility of canine spermatozoa. The protective effect of ZnBPs against the cooling effect may be due to their ability of preventing sperm membrane damage.

Colloid centrifugation removes seminal plasma and cholesterol from boar spermatozoa

2011 ◽  
Vol 23 (7) ◽  
pp. 858 ◽  
Author(s):  
R. Kruse ◽  
P. C. Dutta ◽  
J. M. Morrell
Keyword(s):  
Plasma Membrane ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Single Layer ◽  
Sperm Cryopreservation ◽  
Before And After ◽  
Sperm Plasma Membrane ◽  
Seminal Plasma Proteins ◽  
Boar Spermatozoa ◽  
Layer Chromatography

The objective of the present study was to investigate the effect of Single-Layer Centrifugation (SLC) on boar spermatozoa, namely the effect of removal of seminal plasma proteins and cholesterol from the surface of spermatozoa. The presence of porcine seminal plasma proteins I and II (PSP-I/PSP-II) before and after SLC was studied using immunofluorescence, whereas the removal of cholesterol was shown qualitatively by thin-layer chromatography (TLC). Finally, the integrity of the sperm plasma membrane was observed by electron microscopy. It was shown that the seminal plasma proteins PSP-I and -II were removed from spermatozoa during SLC but could be restored by adding seminal plasma to the SLC-selected sperm samples. Some cholesterol was also lost from the spermatozoa during SLC but the plasma membrane itself appeared to be morphologically intact. Further studies are underway to examine the relevance of these findings to boar sperm cryopreservation and sperm fertility.

Genetic Polymorphism of Boar Seminal Plasma Proteins

1993 ◽  
Vol 64 (3) ◽  
pp. 221-227
Author(s):  
Soichi TSUJI ◽  
Masatoshi ASAO ◽  
Hiroshi KUSUNOKI ◽  
Takao OISHI
Keyword(s):  
Genetic Polymorphism ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Seminal Plasma Proteins

Seasonal differences in seminal plasma proteins from two bovine breeds adapted to a subtropical climate

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Paula Lorena Souto ◽  
Lilian Silveira Travassos Carmouy ◽  
Cristiane Santos ◽  
Edison Martins ◽  
Vera Martins ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Subtropical Climate ◽  
Seasonal Differences ◽  
Seminal Plasma Proteins

scholarly journals Proteins of the canine seminal plasma

2016 ◽  
Vol 46 (5) ◽  
pp. 901-908 ◽  
Author(s):  
Annice Aquino-Cortez ◽  
Lúcia Daniel Machado da Silva ◽  
Airton Alencar de Araújo ◽  
Erika da Silva Bezerra de Menezes ◽  
Arlindo de Alencar Araripe Noronha Moura
Keyword(s):  
Alkaline Phosphatase ◽  
Seminal Plasma ◽  
Binding Proteins ◽  
Reproductive Tract ◽  
Sperm Quality ◽  
Zinc Binding ◽  
Heparin Binding ◽  
Reproductive Disorders ◽  
The Matrix ◽  
Peroxidase Enzymes

ABSTRACT: Studies have been performed to identify the proteins present in canine seminal plasma (SP) and relate them to sperm quality as well as to discover molecular markers of reproductive tract diseases. There is evidence that heparin-binding proteins, zinc-binding proteins, and lactoferrin as well as the matrix metalloproteinase, superoxide dismutase, catalase, and glutathione peroxidase enzymes are associated with canine sperm quality. Other studies indicate that prolactin and enzymes like arginine esterase, acid phosphatase, and alkaline phosphatase could be successfully used as biomarkers of reproductive disorders. Thus, the present literature review aims to address aspects related to proteins of the canine SP, their influence on fertility, and their importance as biomarkers of reproductive disorders.

scholarly journals Seminal Plasma Proteins Revert the Cold-Shock Damage on Ram Sperm Membrane1

2000 ◽  
Vol 63 (5) ◽  
pp. 1531-1537 ◽  
Author(s):  
Beatriz Barrios ◽  
Rosaura Pérez-Pé ◽  
Margarita Gallego ◽  
Agustín Tato ◽  
Jesús Osada ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Cold Shock ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

2007 ◽  
Vol 30 (12) ◽  
pp. 1979-1988 ◽  
Author(s):  
Md Imtaiyaz Hassan ◽  
Vijay Kumar ◽  
Tara Kashav ◽  
Neelima Alam ◽  
Tej P. Singh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Tumor Proliferation ◽  
Proteomic Approach ◽  
Seminal Plasma Proteins
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