scholarly journals Regulation of beta1 integrin expression in endothelial cells by chimeric tRNA(Val) ribozyme.

2006 ◽  
Vol 53 (2) ◽  
pp. 311-316 ◽  
Author(s):  
Izabela Papiewska-Pajak ◽  
Sławomir Antoszczyk
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Line ◽  
Hammerhead Ribozyme ◽  
Human Endothelial Cell ◽  
Integrin Subunit ◽  
Integrin Expression ◽  
Constitutive Transport Element ◽  
Human Endothelial Cell Line ◽  
Beta1 Integrin

To downregulate expression of the beta1 integrin subunit in endothelial cells, plasmid encoding the ribozyme cassette containing hammerhead ribozyme flanked at the 5' terminus by tRNA(Val) and at the 3' terminus by constitutive transport element sequences was constructed. When used to transfect immortalized human endothelial cell line EA.hy 926, it selectively blocked the synthesis of the beta1 integrin subunit and thus inhibited migration and proliferation of the cells. Thus, this construct may be a valuable tool to control the proangiogenic phenotype of stimulated endothelial cells.

Characterization of a human endothelial cell line from a hepatic angiosarcoma

1993 ◽  
Vol 51 ◽  
pp. 14-15
Author(s):  
John M. Hoffpauir ◽  
Marie L. Hoover ◽  
Václav Větvička ◽  
Carlo H. Tamburro
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Line ◽  
Vinyl Chloride ◽  
Coagulation Factor ◽  
Human Endothelial Cell ◽  
Endothelial Cell Line ◽  
Hepatic Angiosarcoma ◽  
Human Endothelial Cell Line

Angiosarcoma of the liver is extremely rare, but there is an increased incidence in Louisville, Kentucky due to worker exposure to gaseous vinyl chloride. The tumor is endothelial in origin as it possess factor VIII, a coagulation factor and known endothelial cell marker. The malignant cells have been described as pleomorphic malignant endothelial cells, malignant spherical and spindle cells or enlarged spindle-shaped endothelial cells. The lack of a cell line derived from a hepatic angiosarcoma has significantly limited studies of this rare lethal malignancy. This study describes the characterization of a human endothelial cell line from a liver biopsy of a hepatic angiosarcoma patient who had been previously exposed to gaseous vinyl chloride. This cell line denoted, HAEND, does not require growth factors such as endothelial cell growth factor for cultivation. Also, this line can be cultivated on untreated plastic surfaces and does not require gelatin or fibronectin coated surfaces.

scholarly journals Candida albicans Expresses a Focal Adhesion Kinase-Like Protein That Undergoes Increased Tyrosine Phosphorylation upon Yeast Cell Adhesion to Vitronectin and the EA.hy 926 Human Endothelial Cell Line

2002 ◽  
Vol 70 (7) ◽  
pp. 3804-3815 ◽  
Author(s):  
Giorgio Santoni ◽  
Roberta Lucciarini ◽  
Consuelo Amantini ◽  
Jordan Jacobelli ◽  
Elisabetta Spreghini ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Candida Albicans ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Yeast Cell ◽  
Focal Adhesion Kinase ◽  
Cell Line ◽  
Tyrosine Phosphorylation ◽  
Focal Adhesion ◽  
Human Endothelial Cell Line

ABSTRACT The signaling pathways triggered by adherence of Candida albicans to the host cells or extracellular matrix are poorly understood. We provide here evidence in C. albicans yeasts of a p105 focal adhesion kinase (Fak)-like protein (that we termed CaFak), antigenically related to the vertebrate p125Fak, and its involvement in integrin-like-mediated fungus adhesion to vitronectin (VN) and EA.hy 926 human endothelial cell line. Biochemical analysis with different anti-chicken Fak antibodies identified CaFak as a 105-kDa protein and immunofluorescence and cytofluorimetric analysis on permeabilized cells specifically stain C. albicans yeasts; moreover, confocal microscopy evidences CaFak as a cytosolic protein that colocalizes on the membrane with the integrin-like VN receptors upon yeast adhesion to VN. The protein tyrosine kinase (PTK) inhibitors genistein and herbimycin A strongly inhibited C. albicans yeast adhesion to VN and EA.hy 926 endothelial cells. Moreover, engagement of αvβ3 and αvβ5 integrin-like on C. albicans either by specific monoclonal antibodies or upon adhesion to VN or EA.hy 926 endothelial cells stimulates CaFak tyrosine phosphorylation that is blocked by PTK inhibitor. A role for CaFak in C. albicans yeast adhesion was also supported by the failure of VN to stimulate its tyrosine phosphorylation in a C. albicans mutant showing normal levels of CaFak and VNR-like integrins but displaying reduced adhesiveness to VN and EA.hy 926 endothelial cells. Our results suggest that C. albicans Fak-like protein is involved in the control of yeast cell adhesion to VN and endothelial cells.

Human endothelial cell line from an angiosarcoma

1993 ◽  
Vol 29 (3) ◽  
pp. 199-202 ◽  
Author(s):  
Marie L. Hoover ◽  
Václav Větvička ◽  
John M. Hoffpauir ◽  
Carlo H. Tamburro
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Human Endothelial Cell ◽  
Endothelial Cell Line ◽  
Human Endothelial Cell Line

Palmitic acid but not palmitoleic acid induces insulin resistance in a human endothelial cell line by decreasing SERCA pump expression

2016 ◽  
Vol 28 (1) ◽  
pp. 53-59 ◽  
Author(s):  
J. Gustavo Vazquez-Jimenez ◽  
Jesus Chavez-Reyes ◽  
Tatiana Romero-Garcia ◽  
Angel Zarain-Herzberg ◽  
Jesus Valdes-Flores ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Endothelial Cell ◽  
Palmitic Acid ◽  
Cell Line ◽  
Palmitoleic Acid ◽  
Human Endothelial Cell ◽  
Endothelial Cell Line ◽  
Human Endothelial Cell Line ◽  
Serca Pump
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