scholarly journals Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana.

2006 ◽  
Vol 53 (2) ◽  
pp. 289-298 ◽  
Author(s):  
Mateusz Wydro ◽  
Edward Kozubek ◽  
Przemysław Lehmann
Keyword(s):  
Gene Expression ◽  
Industrial Production ◽  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Expression System ◽  
Transient Gene Expression ◽  
Maximum Level ◽  
Gene Expression System ◽  
Gfp Gene ◽  
Viral Suppressors

Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 microM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals Transient Gene Expression System Using Protoplasts from Developing Soybean Cotyledons.

1993 ◽  
Vol 43 (1) ◽  
pp. 53-60
Author(s):  
Mayuko ISHIKAWA ◽  
Kyuya HARADA ◽  
Fukumi SAKAI ◽  
Yuko OHASHI ◽  
Tadao NAITO
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Transient Gene Expression ◽  
Gene Expression System

An Agrobacterium-mediated transient gene expression system for intact leaves

Plant Science ◽  
1997 ◽  
Vol 122 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Jyoti Kapila ◽  
Riet De Rycke ◽  
Marc Van Montagu ◽  
Geert Angenon
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Transient Gene Expression ◽  
Gene Expression System ◽  
Intact Leaves
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