scholarly journals Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro.

2003 ◽  
Vol 50 (4) ◽  
pp. 1165-1174 ◽  
Author(s):  
Agnieszka Szurko ◽  
Gabriela Krämer-Marek ◽  
Maria Wideł ◽  
Alicja Ratuszna ◽  
Jan Habdas ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Clonogenic Assay ◽  
Photophysical Properties ◽  
Red Light ◽  
Water Soluble ◽  
Human Malignant Melanoma ◽  
Cytologic Examination ◽  
Inhibition Of Growth ◽  
Toxic Concentrations

Two water soluble porphyrins: meso-tetra-4-N-methylpyridyl-porphyrin iodide (P1) and 5,10-di-(4-acetamidophenyl)-15,20-di-(4-N-methylpyridyl) porphyrin (P2) were synthesised and evaluated in respect to their photochemical and photophysical properties as well as biological activity. Cytotoxic and phototoxic effects were evaluated in human malignant melanoma Me45 line using clonogenic assay, cytological study of micronuclei, apoptosis and necrosis frequency and inhibition of growth of megacolonies. Both porphyrins were characterised by high UV and low visible light absorptions. Dark toxicity measured on the basis of the clonogenic assay and inhibition of megacolony growth area indicated that P1 was non-toxic at concentrations up to 50 microg/ml (42.14 microM) and P2 at concentrations up to 20 microg/ml (16.86 microM). The photodynamic effect induced by red light above 630 nm indicated that both porphyrins were able to inhibit growth of melanoma megacolonies at non-toxic concentrations. Cytologic examination showed that the predominant mode of cell death was necrosis.

scholarly journals Water-soluble cyclometalated platinum(ii) and iridium(iii) complexes: synthesis, tuning of the photophysical properties, and in vitro and in vivo phosphorescence lifetime imaging

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17224-17236 ◽  
Author(s):  
Anastasia I. Solomatina ◽  
Shih-Hao Su ◽  
Maria M. Lukina ◽  
Varvara V. Dudenkova ◽  
Vladislav I. Shcheslavskiy ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Water Soluble ◽  
Iridium Complexes ◽  
Hypoxia Imaging ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

Novel water-soluble iridium complexes with sulfonated diphosphine allow in vitro and in vivo lifetime hypoxia imaging.

scholarly journals Radiation respones of HMV-I human malignant melanoma cells grown in vitro as multicellular spheroids.

1984 ◽  
Vol 25 (3) ◽  
pp. 215-224 ◽  
Author(s):  
NOBUO KUBOTA ◽  
KENGO MATSUI ◽  
SHINICHIRO SATO ◽  
TETSUO INADA
Keyword(s):  
Malignant Melanoma ◽  
Melanoma Cells ◽  
Multicellular Spheroids ◽  
Human Malignant Melanoma

scholarly journals Human Malignant Melanoma-Derived Progestagen-Associated Endometrial Protein Immunosuppresses T Lymphocytes In Vitro

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0119038 ◽  
Author(s):  
Suping Ren ◽  
Lina Chai ◽  
Chunyan Wang ◽  
Changlan Li ◽  
Qiquan Ren ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
T Lymphocytes ◽  
Human Malignant Melanoma

scholarly journals Chemical and Biological Characterization of the Anticancer Potency of Salvia fruticosa in a Model of Human Malignant Melanoma

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2472
Author(s):  
Sotiris Kyriakou ◽  
Venetia Tragkola ◽  
Michael Plioukas ◽  
Ioannis Anestopoulos ◽  
Paschalina S. Chatzopoulou ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Apoptotic Cell ◽  
Total Phenolic ◽  
Activity Levels ◽  
Hacat Cells ◽  
Apoptotic Pathway ◽  
Human Malignant Melanoma ◽  
Anticancer Properties ◽  
Salvia Fruticosa

Malignant melanoma is one of the most aggressive types of skin cancer with an increasing incidence worldwide. Thus, the development of innovative therapeutic approaches is of great importance. Salvia fruticosa (SF) is known for its anticancer properties and in this context, we aimed to investigate its potential anti-melanoma activity in an in vitro model of human malignant melanoma. Cytotoxicity was assessed through a colorimetric-based sulforhodamine-B (SRB) assay in primary malignant melanoma (A375), non-malignant melanoma epidermoid carcinoma (A431) and non-tumorigenic melanocyte neighbouring keratinocyte (HaCaT) cells. Among eight (8) different fractions of S. fruticosa extracts (SF1-SF8) tested, SF3 was found to possess significant cytotoxic activity against A375 cells, while A431 and HaCaT cells remained relatively resistant or exerted no cytotoxicity, respectively. In addition, the total phenolic (Folin–Ciocalteu assay) and total flavonoid content of SF extracts was estimated, whereas the antioxidant capacity was measured via the inhibition of tert-butyl hydroperoxide-induced lipid peroxidation and protein oxidation levels. Finally, apoptotic cell death was assessed by utilizing a commercially available kit for the activation of caspases - 3, - 8 and - 9. In conclusion, the anti-melanoma properties of SF3 involve the induction of both extrinsic and intrinsic apoptotic pathway(s), as evidenced by the increased activity levels of caspases - 8, and - 9, respectively.

Sign in / Sign up

Export Citation Format

Share Document