scholarly journals Protective role of L-methionine against free radical damage of rat brain synaptosomes.

2002 ◽  
Vol 49 (4) ◽  
pp. 907-916 ◽  
Author(s):  
Vyacheslav S Slyshenkov ◽  
Anna A Shevalye ◽  
Anton V Liopo ◽  
Lech Wojtczak
Keyword(s):  
Lipid Peroxidation ◽  
Glutathione Reductase ◽  
Rat Brain ◽  
Glutathione Transferase ◽  
Membrane Damage ◽  
Total Content ◽  
Mitochondrial Fraction ◽  
Lipid Peroxidation Product ◽  
Free Radical Damage ◽  
Tert Butylhydroperoxide

Incubation of rat brain synaptosomal/mitochondrial fraction with tert-butylhydroperoxide resulted in accumulation of the lipid peroxidation product, conjugated dienes, damage of the synaptosomal membrane as evidenced by leakage of lactate dehydrogenase, and decrease of the total content of glutathione and of the GSH/GSSG ratio. This treatment also produced a considerable decrease of the ouabain-sensitive ATPase activity and a much smaller diminution of the activities of glutathione reductase and glutathione transferase. Preincubation of the synaptosomal/mitochondrial fraction with 0.5 or 1.0 mM L-methionine significantly protected against lipid peroxidation, membrane damage and changes in the glutathione system produced by low (1 mM) concentrations of tert-butylhydroperoxide and completely prevented inactivation of ouabain-sensitive ATPase, glutathione reductase and glutathione transferase by such treatment. The importance of L-methionine in antioxidant protection is discussed.

Detection of the lipid peroxidation product malonaldehyde in rat brain in vivo

1995 ◽  
Vol 200 (1) ◽  
pp. 69-72 ◽  
Author(s):  
A.H. Waterfall ◽  
G. Singh ◽  
J.R. Fry ◽  
C.A. Marsden
Keyword(s):  
Lipid Peroxidation ◽  
Rat Brain ◽  
Lipid Peroxidation Product ◽  
Peroxidation Product

scholarly journals Depletion of intracellular glutathione and increased lipid peroxidation mediate cytotoxicity of hematite nanoparticles in MRC-5 cells.

2010 ◽  
Vol 57 (3) ◽  
Author(s):  
Mihaela Radu ◽  
Maria Cristina Munteanu ◽  
Sorina Petrache ◽  
Andreea Iren Serban ◽  
Diana Dinu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Lung Cells ◽  
Lipid Peroxidation Product ◽  
Hematite Nanoparticles ◽  
Mediate Cytotoxicity ◽  
Peroxidation Product

Particles generated from numerous anthropogenic and/or natural sources, such as crystalline α-Fe₂O₃ nanoparticles, have the potential to damage lung cells. In our study we investigated the effects of these nanoparticles (12.5 µg/ml) on lipid peroxidation and the antioxidative system in MRC-5 lung fibroblast cells following exposure for 24, 48 or 72h. Exposure to α-Fe₂O₃ nanoparticles increased lipid peroxidation by 81%, 189% and 110% after 24, 48 and 72h, respectively. Conversely, the reduced glutathione concentration decreased by 23.2% and 51.4% after 48 and 72h of treatment, respectively. In addition, an augmentation of the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione transferase and glutathione reductase within the interval between 48-72h was noticed. Taking into account that the reduced glutathione level decreased and the malondialdehyde level, a lipid peroxidation product, remained highly increased up to 72h of exposure, it would appear that the MRC-5 antioxidant defense mechanisms did not efficiently counteract the oxidative stress induced by exposure to hematite nanoparticles.

scholarly journals Sensitivity of Osteosarcoma Cells to Concentration-Dependent Bioactivities of Lipid Peroxidation Product 4-Hydroxynonenal Depend on Their Level of Differentiation

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 269
Author(s):  
Suzana Borovic Sunjic ◽  
Ana Cipak Gasparovic ◽  
Morana Jaganjac ◽  
Gerald Rechberger ◽  
Andreas Meinitzer ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acid ◽  
Glutathione Transferase ◽  
Biological Effects ◽  
Protein Adducts ◽  
Transferase Activity ◽  
Lipid Peroxidation Product ◽  
Osteosarcoma Cells ◽  
Proteasomal Activity ◽  
Human Osteosarcoma Cells

4-Hydroxynonenal (HNE) is a major aldehydic product of lipid peroxidation known to exert several biological effects. Normal and malignant cells of the same origin express different sensitivity to HNE. We used human osteosarcoma cells (HOS) in different stages of differentiation in vitro, showing differences in mitosis, DNA synthesis, and alkaline phosphatase (ALP) staining. Differentiated HOS cells showed decreased proliferation (3H-thymidine incorporation), decreased viability (thiazolyl blue tetrazolium bromide-MTT), and increased apoptosis and necrosis (nuclear morphology by staining with 4′,6-diamidino-2-phenylindole-DAPI). Differentiated HOS also had less expressed c-MYC, but the same amount of c-FOS (immunocytochemistry). When exposed to HNE, differentiated HOS produced more reactive oxygen species (ROS) in comparison with undifferentiated HOS. To clarify this, we measured HNE metabolism by an HPLC method, total glutathione (GSH), oxidized GSH (ox GSH), glutathione transferase activity (GST), proteasomal activity by enzymatic methods, HNE-protein adducts by genuine ELISA and fatty acid composition by GC-MS in these cell cultures. Differentiated HOS cells had less GSH, lower HNE metabolism, increased formation of HNE-protein adducts, and lower proteasomal activity, in comparison to undifferentiated counterpart cells, while GST and oxGSH were the same. Fatty acids analyzed by GC-MS showed that there is an increase in C20:3 in differentiated HOS while the amount of C20:4 remained the same. The results showed that the cellular machinery responsible for protection against toxicity of HNE was less efficient in differentiated HOS cells. Moreover, differentiated HOS cells contained more C20:3 fatty acid, which might make them more sensitive to free radical-initiated oxidative chain reactions and more vulnerable to the effects of reactive aldehydes such as HNE. We propose that HNE might act as natural promotor of decay of malignant (osteosarcoma) cells in case of their differentiation associated with alteration of the lipid metabolism.

Effect of testosterone and steroids homologues on indolamines and lipid peroxidation in rat brain

2005 ◽  
Vol 94 (4) ◽  
pp. 369-373 ◽  
Author(s):  
David Calderón Guzmán ◽  
Gerardo Barragán Mejía ◽  
Ivonne Espitia Vázquez ◽  
Ernestina Hernández García ◽  
Daniel Santamaría del Angel ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Rat Brain

scholarly journals Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Israel Pérez-Torres ◽  
Verónica Guarner-Lans ◽  
Alejandra Zúñiga-Muñoz ◽  
Rodrigo Velázquez Espejel ◽  
Alfredo Cabrera-Orefice ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Enzymatic Activities ◽  
Glutathione S Transferase ◽  
Control Groups ◽  
Intact Female ◽  
Hormonal Replacement

We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.

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