scholarly journals Expression of beta1-integrins and N-cadherin in bladder cancer and melanoma cell lines.

2000 ◽  
Vol 47 (4) ◽  
pp. 1159-1170 ◽  
Author(s):  
P Laidler ◽  
D Gil ◽  
A Pituch-Noworolska ◽  
D Ciołczyk ◽  
D Ksiazek ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Melanoma Cells ◽  
Integrin Subunit ◽  
Bladder Cell ◽  
Transitional Cell Cancer

Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of alpha5 (P < 0.001), alpha2 and beta1 (P < 0.05 - P < 0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of beta1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of alpha2, alpha3, and alpha5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of alpha2, alpha3 and alpha5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and alpha5 integrin subunit seems to be associated with the most invasive melanoma phenotype.

scholarly journals Do Standardised Prognostic Algorithms Reflect Local Practice? Application of EORTC Risk Tables for Non-Muscle Invasive (pTa/pT1) Bladder Cancer Recurrence and Progression in a Local Cohort

2011 ◽  
Vol 11 ◽  
pp. 751-759 ◽  
Author(s):  
Rajiv Pillai ◽  
Duolao Wang ◽  
Erik K. Mayer ◽  
Paul Abel
Keyword(s):  
Bladder Cancer ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Cancer Recurrence ◽  
Risk Groups ◽  
Original Algorithm ◽  
Transitional Cell Cancer ◽  
Study Population ◽  
Muscle Invasive ◽  
Prognostic Data

A risk calculator algorithm to allow prediction of probabilities of 1- and 5-year recurrence and progression rates in individuals with pTa/pT1 bladder cancer has been proposed by the European Organisation for Research and Treatment of Cancer (EORTC) and was incorporated into the European Association of Urology guidelines in 2006. We attempted to validate this algorithm in a cohort of patients with known outcome. Prognostic data were collected from a consecutively presenting cohort of 109 patients with non-muscle invasive (pTa/pT1) transitional cell cancer (TCC) at a single institution between 1983 and 1985. Using the same statistical models as in the EORTC original paper, predicted probabilities of 1- and 5-year recurrence and progression were calculated. Patients were divided into four risk groups for recurrence (Ir-IVr) and progression (Ip-IVp), respectively, using six prognostic criteria. These were then compared to the probabilities predicted in the EORTC algorithm. The predicted 1- and 5-year probabilities of recurrence were significantly higher in the study population as compared to the original EORTC algorithm for all four risk groups. The predicted 1-year probabilities for progression in groups Ip/IIIp and at 5-years for groups Ip/IIp were in accordance with the original algorithm, but were higher for the other progression groups. The concordance for the model of prediction using the study group for recurrence at 1 and 5 years was 62 and 63%, respectively, and for progression was 65 and 67, respectively. We were unable to validate the proposed algorithm in our group of patients. Although our study has limitations that prevent firm conclusions on the validity of the algorithm, it does expose some of the drawbacks of standardised nomograms when applied to local clinical practice.

Adriamycin (ADM) induced apoptosis in Transitional Cell Cancer (TCC) cell lines accompanied by p21 WAF1/CIP1 induction

APOPTOSIS ◽  
1997 ◽  
Vol 2 (2) ◽  
pp. 207-213 ◽  
Author(s):  
V. N. Bilim ◽  
Y. Tomita ◽  
T. Kawasaki ◽  
M. Takeda ◽  
K. Takahashi
Keyword(s):  
Cell Lines ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Transitional Cell Cancer ◽  
P21 Waf1 ◽  
Induced Apoptosis

scholarly journals Differences of alpha3beta1 integrin glycans from different human bladder cell lines.

2000 ◽  
Vol 47 (2) ◽  
pp. 427-434 ◽  
Author(s):  
A Lityńska ◽  
M Przybyło ◽  
D Ksiazek ◽  
P Laidler
Keyword(s):  
Cell Lines ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Complex Type ◽  
Bladder Epithelium ◽  
Human Bladder ◽  
Bladder Cell ◽  
High Mannose ◽  
Alpha3beta1 Integrin ◽  
Beta1 Subunit

Expression as well as properties of integrins are altered upon transformation. Cell adhesion regulated by integrins is modulated by glycosylation, one of the most frequent biochemical alteration associated with tumorogenesis. Characterisation of carbohydrate moieties of alpha3beta1 integrin on the cultured human bladder carcinoma (T-24, Hu456, HCV 29T) and human normal ureter and bladder epithelium (HCV 29, Hu609) cell lines was carried out after an electrophoresis and blotting, followed by immunochemical identification of alpha3 and beta1 integrin chains and analysis of their carbohydrates moieties using highly specific digoxigenin-labelled lectins. In all the studied cell lines alpha3beta1 integrin was glycosylated although in general each subunit differently. Basic structures recognized in beta1 subunit were tri- or tetraantennary complex type glycans in some cases sialylated (T-24, HCV 29, HCV 29T) and fucosylated (Hu609, HCV 29T). Positive reaction with Phaseolus vulgaris agglutinin and Datura stramonium agglutinin suggesting the presence of beta1-6 branched N-linked oligosaccharides was found in cancerous cell lines (T-24, Hu456) as well as in normal bladder epithelium cells (Hu609). High mannose type glycan was found only in beta1 subunit from Hu456 transitional cell cancer line. On the other hand alpha3 subunit was much less glycosylated except the invasive cancer cell line T-24 where high mannose as well as sialylated tri- or tetraantennary complex type glycans were detected. This observation suggests that changes in glycosylation profile attributed to invasive phenotype are rather associated with alpha3 not beta1 subunit.

Indici di proliferazione (Ki 67, TLI) ed espressione della p53. Introduzione: Proliferation indices (Ki 67, TLI) and p53 expression. Introduction

1995 ◽  
Vol 62 (2) ◽  
pp. 229-233
Author(s):  
R. Minari ◽  
B. Monica
Keyword(s):  
Bladder Cancer ◽  
Prognostic Factors ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Ki 67 ◽  
P53 Expression ◽  
Proliferation Indices ◽  
Cancer Of The Bladder ◽  
Pathologic Findings ◽  
Transitional Cell Cancer

Histologic and pathologic findings are the most common and useful prognostic factors in transitional cell cancer of the bladder. Thus, clinicians know their peculiar limits very well. The Authors report the results of a literature review about an oncogenic-derivative protein (p53) and about indices of proliferation Ki 67 and TLI, which are frequently studied in bladder cancer.

scholarly journals Physiologically Relevant Oxygen Concentration (6% O2) as an Important Component of the Microenvironment Impacting Melanoma Phenotype and Melanoma Response to Targeted Therapeutics In Vitro

2019 ◽  
Vol 20 (17) ◽  
pp. 4203 ◽  
Author(s):  
Marta Osrodek ◽  
Mariusz Hartman ◽  
Malgorzata Czyz
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Oxygen Concentration ◽  
Melanoma Cell ◽  
Atmospheric Oxygen ◽  
Melanoma Cells ◽  
In Vitro Studies ◽  
Cell Phenotype ◽  
Dependent Manner

Cancer cell phenotype largely depends on oxygen availability. The atmospheric oxygen concentration (21%) used in in vitro studies is much higher than in any human tissue. Using well-characterized patient-derived melanoma cell lines, we compared: (i) activities of several signaling pathways, and (ii) the effects of vemurafenib and trametinib in hyperoxia (21% O2), normoxia (6% O2) and hypoxia (1% O2). A high plasticity of melanoma cells in response to changes in oxygen supplementation and drug treatment was observed, and the transcriptional reprograming and phenotypic changes varied between cell lines. Normoxia enhanced the expression of vascular endothelial growth factor (VEGF), glucose metabolism/transport-related genes, and changed percentages of NGFR- and MITF-positive cells in cell line-dependent manner. Increased protein stability might be responsible for high PGC1α level in MITFlow melanoma cells. Vemurafenib and trametinib while targeting the activity of MAPK/ERK pathway irrespective of oxygen concentration, were less effective in normoxia than hyperoxia in reducing levels of VEGF, PGC1α, SLC7A11 and Ki-67-positive cells in cell line-dependent manner. In conclusion, in vitro studies performed in atmospheric oxygen concentration provide different information on melanoma cell phenotype and response to drugs than performed in normoxia, which might partially explain the discrepancies between results obtained in vitro and in clinical settings.

scholarly journals Tulium laser in urothelial carcinoma of the bladder with spread to the ostium of the ureter

2020 ◽  
Vol 16 (3) ◽  
pp. 198-204
Author(s):  
M. E. Novikov ◽  
N. A. Meleshko ◽  
I. G. Akopyan
Keyword(s):  
Bladder Cancer ◽  
Urinary Tract ◽  
Urothelial Cancer ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Upper Urinary Tract ◽  
En Bloc ◽  
Non Invasive ◽  
Transitional Cell Cancer ◽  
Urothelial Bladder

Urothelial transitional cell cancer in developed countries is the 4th most common. In terms of frequency, it is outstripped only by prostate, breast, lung, and colorectal cancer. In the vast majority of cases, urothelial carcinoma develops in the bladder. It accounts for 90—95 % of all cases of transitional cell cancer of the urinary tract. Much less often, in 5—10 % have to deal with its localization in the upper urinary tract (in the calico-pelvic system or ureter). In 17 % of upper urinary tract cancers, bladder cancer is simultaneously diagnosed. The incidence of urothelial transitional cell cancer has increased over the past few decades as a result of improved diagnosis and improved survival of patients with this nosology. The use of modern, high-tech equipment for visualization and direct surgical intervention contributes to such results. The use of laser energy as the main tool for tissue dissection in endoscopic oncourology reveals the undeniable advantages of this method in comparison with the traditional electrosurgery.We present a clinical observation of the diagnosis and surgical treatment of urothelial bladder cancer with invasion of the ureter, performed by en-bloc tulium laser. An operation was performed-transurethral resection of a urothelial bladder tumor with invasion of the ureter, performed by en-bloc tulium laser. Intraoperatively, ureteropieloscopy was performed, and the tumor did not spread to the upper urinary tract beyond the intramural part of the ureter. Thanks to the precision of the action of the tulium laser on the tissue, not resection, but, in fact, dissection of the bladder wall, a high-quality macropreparation was obtained, which made it possible to establish a final diagnosis. Histological conclusion: from the bladder — non-invasive urothelial cancer G2-3; from the mouth of the left ureter — fragments of fibrous tissue lined with urothelium from atypia. 3 months after the operation — no recurrence of urothelial cancer was revealed, which indicates a high quality of the operation.This clinical observation demonstrates the superiority of laser en-bloc dissection compared to the treatment of bladder tumors. Obtaining a macro-product of urothelial transitional cell cancer of the highest quality can contribute to avoiding unjustified radical nephrureterectomies and conducting organ-preserving treatment, for absolute and elective indications. The 1.94 µm Tulium Erbium laser is an effective tool for performing minimally invasive transurethral interventions in non-invasive bladder cancer.

scholarly journals Subpopulations of Stem-like Cells in Side Population Cells from the Human Bladder Transitional Cell Cancer Cell Line T24

2009 ◽  
Vol 37 (3) ◽  
pp. 621-630 ◽  
Author(s):  
Z-F Ning ◽  
Y-J Huang ◽  
T-X Lin ◽  
Y-X Zhou ◽  
C Jiang ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Side Population ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Cancer Cell Line ◽  
Specific Cell ◽  
Side Population Cells ◽  
Transitional Cell Cancer ◽  
Specific Cell Surface

Cancer stem cells can be isolated from human tumours using specific cell surface markers. Bladder cancer cells, however, lack specific cell surface markers, making this approach impracticable. In this study an alternative method was used, involving isolation of side population cells to explore the stem cell characteristics of bladder cancer. Side population cells were isolated from the bladder transitional cell cancer cell line T24 and examined for potential stem cell characteristics related to proliferation, cell cycle distribution, self-renewal and differentiation. It was observed that T24 side population cells have stronger proliferative and colony formation abilities than non-side population cells. Side population cells were also more resistant to chemotherapy and radiotherapy, which may be due to expression of the ATP-binding cassette half-transporter, sub-family G, member 2 protein. Overall, the results suggest that side population cells from the human bladder transitional cell cancer cell line T24 harbour stem-like cells.

scholarly journals Data-independent Acquisition-based Proteome and Phosphoproteome Profiling across Six Melanoma Cell Lines Reveals Determinants of Proteotypes

2020 ◽  
Author(s):  
Erli Gao ◽  
Wenxue Li ◽  
Chongde Wu ◽  
Wenguang Shao ◽  
Yi Di ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Human Cancer ◽  
Melanoma Cells ◽  
Single Shot ◽  
Human Cancer Cell Lines ◽  
Data Independent Acquisition ◽  
Biological Studies ◽  
Free Data

ABSTRACTHuman cancer cell lines are widely used in pharmacological and systems biological studies. The rapid documentation of the steady-state gene expression landscape of the cells used in a particular experiment may help to improve the reproducibility of scientific research. Here we applied a data-independent acquisition mass spectrometry (DIA-MS) method, coupled with a peptide spectral-library free data analysis workflow, to measure both proteome and phosphoproteome of a melanoma cell line panel with different metastatic properties. For each cell line, the single-shot DIA-MS detected 8,100 proteins and almost 40,000 phosphopeptides in the respective measurement of two hours. Benchmarking the DIA-MS data towards the RNA-seq data and tandem mass tag (TMT)-MS results from the same set of cell lines demonstrated comparable qualitative coverage and quantitative reproducibility. Our data confirmed the high but complex mRNA~protein and protein~phospsite correlations. The results successfully established DIA-MS as a strong and competitive proteotyping approach for cell lines. The data further showed that all subunits of Glycosylphosphatidylinositol (GPI)-anchor transamidase complex were overexpressed in metastatic melanoma cells and identified altered phosphoprotein modules such as BAF complex and mRNA splicing between metastatic and primary cells. This study provides a high-quality resource for calibrating DIA-MS performance, benchmarking DIA bioinformatic algorithms, and exploring the metastatic proteotypes in melanoma cells.

Characterization of a Cell Line From Human Transitional Cell Cancer of the Urinary Tract 2

1974 ◽  
Vol 53 (5) ◽  
pp. 1341-1349 ◽  
Author(s):  
A. Y. Elliott ◽  
P. Cleveland ◽  
J. Cervenka ◽  
A. E. Castro ◽  
Nell Stein ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Cell Line ◽  
Cell Cancer ◽  
Transitional Cell ◽  
Transitional Cell Cancer ◽  
A Cell
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