scholarly journals Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L.

1998 ◽  
Vol 45 (2) ◽  
pp. 621-626 ◽  
Author(s):  
J Stano ◽  
P Nemec ◽  
L Bezáková ◽  
D Kákoniová ◽  
P Kovács ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Immobilized Cells ◽  
Tween 80 ◽  
Ph Optimum ◽  
Cucumis Sativus L ◽  
Long Term Storage ◽  
High Beta ◽  
Beta Galactosidase ◽  
Term Storage

Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. Beta-galactosidase showed pH optimum at 4.9 and temperature optimum at 58 degrees C. The enzyme catalysed hydrolysis was linear for 3 h with 60-68% conversion of the substrate. The cells characterized by high beta-galactosidase activity and stability on long-term storage showed valuable technological properties.

scholarly journals Degradation of chlorobenzene and 2-chlorotoluene by immobilized bacteria strains Comamonas testosterone kt5 and Bacillus subtilis dkt

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Ha Danh Duc ◽  
Nguyen Thi Oanh
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Strain ◽  
Immobilized Cells ◽  
Resting Cells ◽  
Long Term Storage ◽  
Degradation Rates ◽  
Contributory Negligence ◽  
Biofilm Bacteria ◽  
Term Storage

Chlorobenzenes and chlorotoluenes have been used to produce a number of industrial products. They are toxic and widely detected in environments due to human contributory negligence. In this article, the mixed culture of a toluenes-degrading bacterial strain, Comamonas testosterone KT5 (a Gram-positive, catalase-positive bacterium) and a chlorobenzenes-degrading bacterial strain, Bacillus subtilis DKT (a Gram-negative soil bacterium) effectively degraded both chemical compounds co-contaminating in liquid media. In addition, the degradations of mixed compounds by biofilm, bacteria immobilized in polyurethane foam (PUF) and alginate were determined. The results showed that the degradation of both compounds by cells in alginate was significantly higher than that by suspended cells. Moreover, cells immobilized in these materials showed lower adverse effects than those of non-immobilized cells for long-term storage. For examples, the degradation rates for chlorobenzine and 2-chlorotoluene by resting cells reduced by 39.5% and 37.3% after storage for 4 months at 4°C, while the degradation rates by immobilized cells decreased by from 16.3% to 19.8% respectively. 

Effects of Tween 80 and Sucrose on Acute Short-Term Stability and Long-Term Storage at −20 C of a Recombinant Hemoglobin

1998 ◽  
Vol 87 (9) ◽  
pp. 1062-1068 ◽  
Author(s):  
Bruce A. Kerwin ◽  
Martin C. Heller ◽  
Steven H. Levin ◽  
Theodore W. Randolph
Keyword(s):  
Tween 80 ◽  
Short Term ◽  
Term Stability ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

Catalysts ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 326 ◽  
Author(s):  
Olga Senko ◽  
Marina Gladchenko ◽  
Olga Maslova ◽  
Elena Efremenko
Keyword(s):  
Clostridium Acetobutylicum ◽  
Immobilized Cells ◽  
Poly Vinyl Alcohol ◽  
Anaerobic Sludge ◽  
Lignocellulosic Waste ◽  
Long Term Storage ◽  
Anaerobic Consortium ◽  
High Level ◽  
Term Storage

The aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrifos or methiocarb and its derivatives). It was established that the immobilized cells of the anaerobic consortium can be stored frozen for at least three years while preserving a high level of metabolic activity. The cells after the long-term storage in an immobilized and frozen state were applied for the methanogenesis of a wide number of wastes, and an increase in both methane yield and methane portion in the produced biogas as compared to the conventionally used suspended anaerobic sludge cells, was ensured. It was shown that the “additional” introduction of bacterial Clostridium acetobutylicum, Pseudomonas sp., Enterococcus faecalis cells (also immobilized using same support) improves characteristics of methanogenesis catalyzed by immobilized anaerobic sludge.

scholarly journals PHYTOHORMONAL ACTIVITY OF IMMOBILIZED CELLS OF SALT-TOLERANT COTTON RHIZOBACTERIA AFTER LONG TERM STORAGE

2020 ◽  
pp. 3-5
Author(s):  
Zakiryaeva Saidakhon Ikramovna
Keyword(s):  
Immobilized Cells ◽  
Free Form ◽  
Salt Tolerant ◽  
Long Term Storage ◽  
Free Cells ◽  
Term Storage ◽  
Resistant Cells

The phytohormonal activity of free and immobilized cells of B. subtilis BS-80, B. licheniformis BL-83, P. polymyxa PP-113 and P. amylolyticus PA-118 on phosphate flour have been studied. It was found that immobilized cells more actively synthesized IAA and gibberellins in comparison with free cells. The data obtained indicates that the phytohormonal activity of the salt-resistant cells of cotton rhizobacteria can be kept much better in the immobilized form than in the free form (in a refrigerator on jambs).

scholarly journals “Nature-like” Cryoimmobilization of Phototrophic Microorganisms: New Opportunities for Their Long-Term Storage and Sustainable Use

2022 ◽  
Vol 14 (2) ◽  
pp. 661
Author(s):  
Olga Senko ◽  
Nikolay Stepanov ◽  
Olga Maslova ◽  
Elena Efremenko
Keyword(s):  
Immobilized Cells ◽  
Sustainable Use ◽  
Biomass Accumulation ◽  
Poly Vinyl Alcohol ◽  
High Concentration ◽  
Long Term Storage ◽  
Phototrophic Microorganisms ◽  
High Level ◽  
Term Storage

It was found that immobilization of cells in poly(vinyl alcohol) (PVA) cryogel can be successfully applied for concurrent cryoimmobilization, cryoconservation and long-term storage of the cells of various phototrophic microorganisms (green and red microalgae, diatoms and cyanobacteria). For the first time, it was shown for 12 different immobilized microalgal cells that they can be stored frozen for at least 18 months while retaining a high level of viability (90%), and can further be used as an inoculum upon defrosting for cell-free biomass accumulation. Application of cryoimmobilized Chlorella vulgaris cells as inocula allowed the loading of a high concentration of the microalgal cells into the media for free biomass accumulation, thus increasing the rate of the process. It was shown that as minimum of 5 cycles of reuse of the same immobilized cells as inocula for cell accumulation could be realized when various real wastewater samples were applied as media for simultaneous microalgae cultivation and water purification.

scholarly journals Formulation and Characterization of Kaffir Lime Oil Nanoemulsion

2017 ◽  
Vol 14 (3) ◽  
pp. 915-922
Author(s):  
Ghea Putri Christy ◽  
Dewa Ayu Arimurni ◽  
Made Dwi Pradipta Wahyudi ◽  
Ronny Martien ◽  
Woro Anindito Sri Tunjung
Keyword(s):  
Essential Oil ◽  
Propylene Glycol ◽  
Tween 80 ◽  
Colloidal Systems ◽  
Spontaneous Emulsification ◽  
Low Viscosity ◽  
Long Term Storage ◽  
Term Storage

ABSTRACT: Kaffir lime oil has many health benefits. However, an obstacle to its commercial use is oxidation during storage. Nanoemulsions (particulate colloidal systems) have been shown to be suitable carriers for lipophilic essential oil constituents due to amphipathic compounds that facilitate solubility. The objectives of this study were to formulate thermodynamically stable kaffir lime oil nanoemulsions and to investigate their physicochemical properties. Air-dried leaves of kaffir lime were subjected to steam distillation to obtain essential oil. Preparation of nanoemulsions was done using the spontaneous emulsification method. Tween 80 and propylene glycol were selected as surfactant mix components. The oil phase consisted of Miglyol 812 as a carrier oil for kaffir lime oil while double-distilled water was used in the aqueous phase. The best formula with transmittance above 95% and highest essential oil content was selected. It contained 20% of Tween 80, 10% of propylene glycol, 1.25% Miglyol 812, and 3.75% kaffir lime essential oil. This formula was then characterized and its thermodynamic stability determined. . The results showed that kaffir lime oil nanoemulsions were thermodynamically stable and robustly withstood variations in temperature, centrifugation, and long-term storage. Additionally, the nanoemulsions had low viscosity, which may facilitate its development as a pharmaceutical compound.

scholarly journals The Effect of Long-Term Storage on Mycobacterium bovis

2021 ◽  
Vol 70 (3) ◽  
pp. 327-337
Author(s):  
OLEXIY TKACHENKO ◽  
NATALI KOZAK ◽  
MARYNA BILAN ◽  
VOLODYMYR HLEBENIUK ◽  
NATALIA ALEKSEEVA ◽  
...  
Keyword(s):  
Low Temperature ◽  
Mycobacterium Bovis ◽  
Nutrient Medium ◽  
Tween 80 ◽  
Initial Strain ◽  
Genetic Changes ◽  
Long Term Storage ◽  
Solid Nutrient Medium ◽  
Term Storage

It was established that when stored for many years (10–13 years) in low-temperature conditions (3°C), without sub-culture on a nutrient medium, Mycobacterium bovis grew as visible colonies along the line of inoculation. However, due to long-term storage in conditions of low temperature (3°C) morphology of mycobacteria differed significantly from initial cultures formed by rod-shaped bacteria. Some of them became pigment-forming and smooth on the surface. Unlike the initial strain of mycobacteria, a perennial bacteria stored under hard conditions did not cause the death of guinea pigs or their sensitization to a purified protein derivative for mammals. Morphological forms of the perennial mycobacteria had the following changes: pigment forming, L-forms of the vesicular type, non-acid-fast thread-like (filamentous) bacillary forms, and elementary bodies when compared to the initial strain. There were also some genetic changes in the target DNA due to the long-term storage of M. bovis. It may indicate a mutation in the pathogen’s DNA. These mycobacteria had altered biochemical activity during storage. The number of passages on the solid nutrient medium did not affect their fermentative activity. However, the low cultivation temperature increases mycobacterial catalase activity and the ability to hydrolyze Tween-80.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
Sign in / Sign up

Export Citation Format

Share Document