scholarly journals DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver.

1995 ◽  
Vol 42 (2) ◽  
pp. 145-151 ◽  
Author(s):  
A Ferraro ◽  
M Eufemi ◽  
L Cervoni ◽  
F Altieri ◽  
C Turano
Keyword(s):  
Dna Sequences ◽  
Nuclear Matrix ◽  
Experimental Approach ◽  
High Sensitivity ◽  
Matrix Proteins ◽  
Dna Fragments ◽  
Dna Interactions ◽  
Nuclear Matrix Proteins ◽  
Matrix Interactions ◽  
The Cross

To detect the interactions of DNA with the nuclear matrix proteins, DNA-protein cross-linkages were induced in intact nuclei from chicken liver by the use of cis-diammine dichloroplatinum. Methods have been devised for fast purification both of the proteins and of the DNA fragments involved in the cross-linked complexes. By Southern-Western blotting a number of matrix proteins isolated from the complexes have been shown to recognize specifically DNA sequences present in the cross-linked DNA fragments. This experimental approach not only allows to identify the nuclear matrix-DNA interactions existing in the nucleus before its disruption, but also provides a preparation of matrix proteins enriched in those species which are involved in such interactions and which can therefore be detected with high sensitivity.

scholarly journals Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression

2008 ◽  
Vol 60 (3) ◽  
pp. 355-366
Author(s):  
Svetlana Dinic ◽  
Mirjana Mihailovic ◽  
Svetlana Ivanovic-Matic ◽  
Aleksandra Uskokovic ◽  
Nevena Grdovic ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Rat Liver ◽  
Dna Sequences ◽  
Nuclear Matrix ◽  
Gene Promoter ◽  
Immunoblot Analysis ◽  
Matrix Proteins ◽  
Nuclear Matrix Proteins ◽  
Matrix Interactions ◽  
Gene Regulatory

Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (?2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the ?2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBP?, and STAT5b were involved in basal and C/EBP?, STAT1, and STAT3 in AP-stimulated ?2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences.

scholarly journals DNA and proteins of the nuclear matrix are the main targets of benzo[a]pyrene's action in rat hepatocytes.

1993 ◽  
Vol 40 (4) ◽  
pp. 559-562 ◽  
Author(s):  
P Widłak ◽  
J Rzeszowska-Wolny
Keyword(s):  
Molecular Weight ◽  
Dna Adducts ◽  
Dna Sequences ◽  
Nuclear Matrix ◽  
Rat Hepatocytes ◽  
The Other ◽  
Matrix Proteins ◽  
Nuclear Matrix Proteins ◽  
Cultured Rat Hepatocytes ◽  
High Molecular Weight Proteins

The binding of [14C]benzo[a]pyrene (B[a]P) to DNA and proteins in total nuclei and subnuclear fractions of cultured rat hepatocytes was compared. The main targets of B[a]P were non-histone high molecular weight proteins of the nuclear matrix and DNA sequences attached to this structure. Following 24 h exposure to B[a]P the amounts of adducts in the nuclear matrix DNA and proteins were twice as high as in total nuclei. After withdrawal of the carcinogen containing medium the level of B[a]P-induced adducts gradually decreased but always remained the highest in the nuclear matrix proteins. Removal of adducts from the nuclear matrix DNA was more efficient than from the other DNA fractions, and 72 h after exposure to the carcinogen the level of DNA adducts in this fraction was similar to that in total nuclei.

Differentiation-Specific Nuclear Matrix Proteins Cross-linked to DNA bycis-Diammine Dichloroplatinum

1998 ◽  
Vol 238 (1) ◽  
pp. 216-219 ◽  
Author(s):  
Elena Mattia ◽  
Margherita Eufemi ◽  
Silvia Chichiarelli ◽  
Mara Ceridono ◽  
Anna Ferraro
Keyword(s):  
Nuclear Matrix ◽  
Matrix Proteins ◽  
Nuclear Matrix Proteins

scholarly journals Interactions of rat repetitive sequence MspI8 with nuclear matrix proteins during spermatogenesis.

1996 ◽  
Vol 43 (2) ◽  
pp. 319-324
Author(s):  
J Rogoliński ◽  
P Widłak ◽  
J Rzeszowska-Wolny
Keyword(s):  
Nuclear Matrix ◽  
Blot Analysis ◽  
Repetitive Sequence ◽  
Matrix Proteins ◽  
Velocity Sedimentation ◽  
Nuclear Matrix Proteins ◽  
Rat Testis ◽  
Sedimentation Technique

Using the Southwestern blot analysis we have studied the interactions between rat repetitive sequence MspI8 and the nuclear matrix proteins of rat testis cells. Starting from 2 weeks the young to adult animals showed differences in type of testis nuclear matrix proteins recognizing the MspI8 sequence. The same sets of nuclear matrix proteins were detected in some fractions enriched in spermatocytes and spermatides and obtained after fractionation of testis cells of adult animals by the velocity sedimentation technique.

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