Facilitating Agricultural Trade in Asia and the Pacific

The thirty-seventh annual meeting of the Philological Association of the Pacific Coast was held at Stanford University, California, on November 29 and 30, 1935.

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A scanning electron microscopic study of the veliger larvae of the scallop argopecten purpuratus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103486 ◽

1988 ◽

Vol 46 ◽

pp. 284-285

Author(s):

G.C. Bellolio ◽

K.S. Lohrmann ◽

E.M. Dupré

Keyword(s):

Morphological Description ◽

Electron Microscopic ◽

Larval Stages ◽

Scanning Electron Microscopic Study ◽

The Pacific ◽

Argopecten Purpuratus ◽

Veliger Larvae ◽

20 Nm ◽

Ethanol Series ◽

Scanning Electron

Argopecten purpuratus is a scallop distributed in the Pacific coast of Chile and Peru. Although this species is mass cultured in both countries there is no morphological description available of the development of this bivalve except for few characterizations of some larval stages described for culture purposes. In this work veliger larvae (app. 140 pm length) were examined by the scanning electron microscope (SEM) in order to study some aspects of the organogenesis of this species.Veliger larvae were obtained from hatchery cultures, relaxed with a solution of MgCl2 and killed by slow addition of 21 glutaraldehyde (GA) in seawater (SW). They were fixed in 2% GA in calcium free artificial SW (pH 8.3), rinsed 3 times in calcium free SW, and dehydrated in a graded ethanol series. The larvae were critical point dried and mounted on double scotch tape (DST). To permit internal view, some valves were removed by slightly pressing and lifting the tip of a cactus spine wrapped with DST, The samples were coated with 20 nm gold and examined with a JEOL JSM T-300 operated at 15 KV.

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