scholarly journals A common basis for facilitated legitimate exchange of biological materials proposed by the European Culture Collections' Organisation

2009 ◽  
Vol 4 (1) ◽  
pp. 507 ◽  
Author(s):  
Dagmar Fritze
Keyword(s):  
Biological Materials ◽  
Culture Collections ◽  
Common Basis ◽  
European Culture

scholarly journals Interoperability of CABRI Services and Biochemical Pathways Databases

2004 ◽  
Vol 5 (2) ◽  
pp. 169-172 ◽  
Author(s):  
Paolo Romano ◽  
Ottavia Aresu ◽  
Maria Assunta Manniello ◽  
Barbara Parodi
Keyword(s):  
Culture Collections ◽  
Biochemical Pathways ◽  
Related Information ◽  
Biological Resource Centres ◽  
Quality Guidelines ◽  
European Culture ◽  
One Stop ◽  
Set Up ◽  
Search Facility ◽  
Simple Search

Common Access to Biological Resources and Information (CABRI) service is a ‘one-stop-shop’ for materials that are collected by a number of European culture collections that engage themselves in a quality service for the scientific community by adhering to Quality Guidelines for the management of resources and related information. It includes collections' catalogues that can be searched in an SRS implementation. A simple search facility, including a synonym search and a shopping cart, is also available. Within the European Biological Resource Centres Network (EBRCN) project, an extension and improvement of the catalogues' information is under way. This includes adding links to bibliographic databanks and sequence databases. Revision of ‘in-house’ controlled vocabularies used by data annotators is under way, in order to improve the setting up of external links, and new links to biochemical pathways databases are being set up for some of the catalogues.

scholarly journals Public Microbial Resource Centers: Key Hubs for Findable, Accessible, Interoperable, and Reusable (FAIR) Microorganisms and Genetic Materials

2019 ◽  
Vol 85 (21) ◽  
Author(s):  
P. Becker ◽  
M. Bosschaerts ◽  
P. Chaerle ◽  
H.-M. Daniel ◽  
A. Hellemans ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Scientific Research ◽  
Biological Materials ◽  
Open Science ◽  
Future Research ◽  
Culture Collections ◽  
Dna Libraries ◽  
Funding Agencies ◽  
Journal Policies ◽  
The Impact

ABSTRACT In the context of open science, the availability of research materials is essential for knowledge accumulation and to maximize the impact of scientific research. In microbiology, microbial domain biological resource centers (mBRCs) have long-standing experience in preserving and distributing authenticated microbial strains and genetic materials (e.g., recombinant plasmids and DNA libraries) to support new discoveries and follow-on studies. These culture collections play a central role in the conservation of microbial biodiversity and have expertise in cultivation, characterization, and taxonomy of microorganisms. Information associated with preserved biological resources is recorded in databases and is accessible through online catalogues. Legal expertise developed by mBRCs guarantees end users the traceability and legality of the acquired material, notably with respect to the Nagoya Protocol. However, awareness of the advantages of depositing biological materials in professional repositories remains low, and the necessity of securing strains and genetic resources for future research must be emphasized. This review describes the unique position of mBRCs in microbiology and molecular biology through their history, evolving roles, expertise, services, challenges, and international collaborations. It also calls for an increased deposit of strains and genetic resources, a responsibility shared by scientists, funding agencies, and publishers. Journal policies requesting a deposit during submission of a manuscript represent one of the measures to make more biological materials available to the broader community, hence fully releasing their potential and improving openness and reproducibility in scientific research.

Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections

2015 ◽  
Vol 99 (8) ◽  
pp. 3559-3571 ◽  
Author(s):  
Jindrich Peiren ◽  
Joke Buyse ◽  
Paul De Vos ◽  
Elke Lang ◽  
Dominique Clermont ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Storage Stability ◽  
Culture Collections ◽  
European Culture ◽  
And Storage

scholarly journals Biosecurity responsibilities of biological resource centres

2006 ◽  
Vol 27 (1) ◽  
pp. 19
Author(s):  
Ronald M Atlas
Keyword(s):  
Public Health ◽  
Economic Development ◽  
Biological Materials ◽  
Health Protection ◽  
Microbial Culture ◽  
Biological Resource ◽  
Culture Collections ◽  
Biological Resource Centres ◽  
The World ◽  
Public Health Protection

Biological resource centres (BRCs), which house the world?s microbial culture collections, have emerging biosecurity responsibilities to prevent terrorists from acquiring dangerous pathogens that could be used to do harm. This presents a major challenge to the primary functions of BRCs, which are to supply biological materials for research, public health protection and economic development.

scholarly journals European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool for This Rarely Occurring Potato Pathogen

2021 ◽  
Vol 9 (4) ◽  
pp. 781
Author(s):  
Jérémy Cigna ◽  
Angélique Laurent ◽  
Malgorzata Waleron ◽  
Krzysztof Waleron ◽  
Pauline Dewaegeneire ◽  
...  
Keyword(s):  
Soft Rot ◽  
European Population ◽  
Genomic Diversity ◽  
Taqman Assay ◽  
Culture Collections ◽  
Strain Characterization ◽  
Detection Tool ◽  
European Culture ◽  
Specific Strain ◽  
Long Time

Enterobacteria belonging to the Pectobacterium and Dickeya genera are responsible for soft rot and blackleg diseases occurring in many crops around the world. Since 2016, the number of described species has more than doubled. However, some new species, such as Pectobacterium punjabense, are often poorly characterized, and little is known about their genomic and phenotypic variation. Here, we explored several European culture collections and identified seven strains of P. punjabense. All were collected from potato blackleg symptoms, sometimes from a long time ago, i.e., the IFB5596 strain isolated almost 25 years ago. We showed that this species remains rare, with less than 0.24% of P. punjabense strains identified among pectinolytic bacteria present in the surveyed collections. The analysis of the genomic diversity revealed the non-clonal character of P. punjabense species. Furthermore, the strains showed aggressiveness differences. Finally, a qPCR Taqman assay was developed for rapid and specific strain characterization and for use in diagnostic programs.

scholarly journals An Information System for European culture collections: the way forward

SpringerPlus ◽  
2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Serge Casaregola ◽  
Alexander Vasilenko ◽  
Paolo Romano ◽  
Vincent Robert ◽  
Svetlana Ozerskaya ◽  
...  
Keyword(s):  
Information System ◽  
Culture Collections ◽  
European Culture ◽  
The Way

New Design for a Differentially Pumped Hydration Chamber for a Siemens IA

1971 ◽  
Vol 29 ◽  
pp. 44-45
Author(s):  
R. C. Moretz ◽  
G. G. Hausner ◽  
D. F. Parsons
Keyword(s):  
Electron Microscopy ◽  
Water Vapor ◽  
Electron Diffraction ◽  
Water Vapor Pressure ◽  
Biological Materials ◽  
Thin Membrane ◽  
Reliable System ◽  
System A ◽  
Water Films ◽  
Aperture Opening

Electron microscopy and diffraction of biological materials in the hydrated state requires the construction of a chamber in which the water vapor pressure can be maintained at saturation for a given specimen temperature, while minimally affecting the normal vacuum of the remainder of the microscope column. Initial studies with chambers closed by thin membrane windows showed that at the film thicknesses required for electron diffraction at 100 KV the window failure rate was too high to give a reliable system. A single stage, differentially pumped specimen hydration chamber was constructed, consisting of two apertures (70-100μ), which eliminated the necessity of thin membrane windows. This system was used to obtain electron diffraction and electron microscopy of water droplets and thin water films. However, a period of dehydration occurred during initial pumping of the microscope column. Although rehydration occurred within five minutes, biological materials were irreversibly damaged. Another limitation of this system was that the specimen grid was clamped between the apertures, thus limiting the yield of view to the aperture opening.

Evaluation of Freezing Methods by Low Temperature X-Ray Diffraction

1977 ◽  
Vol 35 ◽  
pp. 330-333
Author(s):  
T. Gulik-Krzywicki ◽  
M.J. Costello
Keyword(s):  
Biological Materials ◽  
Molecular Organization ◽  
X Ray Diffraction ◽  
Glass Capillary ◽  
X Ray ◽  
Rate Dependent ◽  
Before And After ◽  
Freeze Etching ◽  
Diffraction Patterns ◽  
Set Up

Freeze-etching electron microscopy is currently one of the best methods for studying molecular organization of biological materials. Its application, however, is still limited by our imprecise knowledge about the perturbations of the original organization which may occur during quenching and fracturing of the samples and during the replication of fractured surfaces. Although it is well known that the preservation of the molecular organization of biological materials is critically dependent on the rate of freezing of the samples, little information is presently available concerning the nature and the extent of freezing-rate dependent perturbations of the original organizations. In order to obtain this information, we have developed a method based on the comparison of x-ray diffraction patterns of samples before and after freezing, prior to fracturing and replication.Our experimental set-up is shown in Fig. 1. The sample to be quenched is placed on its holder which is then mounted on a small metal holder (O) fixed on a glass capillary (p), whose position is controlled by a micromanipulator.

Enhanced information from biological materials through technological improvements in cryo-electron microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 788-789
Author(s):  
Marc J.C. de Jong ◽  
Wim M. Busing ◽  
Max T. Otten
Keyword(s):  
Electron Microscopy ◽  
Electron Beam ◽  
Biological Materials ◽  
Electron Crystallography ◽  
Cryo Electron Microscopy ◽  
Transmission Electron ◽  
The Many ◽  
Technological Improvements ◽  
Beam Damage

Biological materials damage rapidly in the electron beam, limiting the amount of information that can be obtained in the transmission electron microscope. The discovery that observation at cryo temperatures strongly reduces beam damage (in addition to making it unnecessaiy to use chemical fixatives, dehydration agents and stains, which introduce artefacts) has given an important step forward to preserving the ‘live’ situation and makes it possible to study the relation between function, chemical composition and morphology.Among the many cryo-applications, the most challenging is perhaps the determination of the atomic structure. Henderson and co-workers were able to determine the structure of the purple membrane by electron crystallography, providing an understanding of the membrane's working as a proton pump. As far as understood at present, the main stumbling block in achieving high resolution appears to be a random movement of atoms or molecules in the specimen within a fraction of a second after exposure to the electron beam, which destroys the highest-resolution detail sought.

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