scholarly journals Caspase 3 activity in isolated fetal rat lung fibroblasts and rat periodontal ligament fibroblasts: cigarette smoke-induced alterations

2016 ◽  
Vol 2 (April Supplement) ◽  
Author(s):  
James Elliot Scott
Keyword(s):  
Cigarette Smoke ◽  
Periodontal Ligament ◽  
Caspase 3 ◽  
Lung Fibroblasts ◽  
Rat Lung ◽  
Periodontal Ligament Fibroblasts ◽  
Fetal Rat ◽  
Fetal Rat Lung

scholarly journals Caspase 3 activity in isolated fetal rat lung fibroblasts and rat periodontal ligament fibroblasts: cigarette smoke induced alterations

2013 ◽  
Vol 11 (1) ◽  
pp. 25 ◽  
Author(s):  
Asra Ahmed ◽  
James A Thliveris ◽  
Anthony Shaw ◽  
Michael Sowa ◽  
James Gilchrist ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Periodontal Ligament ◽  
Caspase 3 ◽  
Lung Fibroblasts ◽  
Rat Lung ◽  
Periodontal Ligament Fibroblasts ◽  
Fetal Rat ◽  
Fetal Rat Lung

scholarly journals Cigarette Smoke Induces Apoptosis by Activation of Caspase-3 in Isolated Fetal Rat Lung Type II Alveolar Ep-ithelial Cells <i>in Vitro</i>

2013 ◽  
Vol 03 (01) ◽  
pp. 4-12 ◽  
Author(s):  
Asra Ahmed ◽  
James A. Thliveris ◽  
Anthony Shaw ◽  
Michael Sowa ◽  
James Gilchrist ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Caspase 3 ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

168 THY-1 EXPRESSION IS ASSOCIATED WITH UP-REGULATION OF WNT SIGNALING AND MYOFIBROBLAST PHENOTYPE IN FETAL RAT LUNG FIBROBLASTS.

2006 ◽  
Vol 54 (1) ◽  
pp. S109.2-S109
Author(s):  
C. Dasgupta ◽  
Y. Wang ◽  
R. Sakurai ◽  
J. Santos ◽  
J. S. Torday ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Lung Fibroblasts ◽  
Rat Lung ◽  
Myofibroblast Phenotype ◽  
Fetal Rat ◽  
Fetal Rat Lung

The adenylate cyclase response to parathyroid hormone in fetal lung fibroblasts is enhanced by cortisol

1987 ◽  
Vol 7 (6) ◽  
pp. 503-508 ◽  
Author(s):  
Carolyn Lowe ◽  
Peter M. Barling ◽  
Stephen J. M. Skinner
Keyword(s):  
Parathyroid Hormone ◽  
Epithelial Cells ◽  
Adenylate Cyclase ◽  
Fetal Lung ◽  
Lung Fibroblasts ◽  
Rat Lung ◽  
Osteosarcoma Cells ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Umr 106

Parathyroid hormone (PTH, <10−8 M) stimulated adenylate cyclase in fibroblasts, but not epithelial cells, isolated from fetal rat lung. In contrast to osteosarcoma cells (UMR 106), the response of fibroblasts to PTH was increased by pretreatment with cortisol (< 10−8-10−7 M).

Differential Regulation of Platelet-Derived Growth Factor Genes in Fetal Rat Lung Fibroblasts

1994 ◽  
Vol 211 (1) ◽  
pp. 142-149 ◽  
Author(s):  
Shilpa Buch ◽  
Colin Jones ◽  
Jason Liu ◽  
Robin N.N. Han ◽  
A.Keith Tanswell ◽  
...  
Keyword(s):  
Growth Factor ◽  
Differential Regulation ◽  
Platelet Derived Growth Factor ◽  
Lung Fibroblasts ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Growth Factor Genes

scholarly journals The ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells

2007 ◽  
Vol 1772 (7) ◽  
pp. 737-747 ◽  
Author(s):  
Washa Liu ◽  
Katja Zscheppang ◽  
Sandy Murray ◽  
Heber C. Nielsen ◽  
Christiane E.L. Dammann
Keyword(s):  
Lung Fibroblasts ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Erbb4 Receptor ◽  
Fetal Rat Lung

Identification of Betamethasone-Regulated Target Genes and Cell Pathways in Fetal Rat Lung Mesenchymal Fibroblasts

Endocrinology ◽  
2019 ◽  
Vol 160 (8) ◽  
pp. 1868-1884 ◽  
Author(s):  
Bennet K L Seow ◽  
Annie R A McDougall ◽  
Kelly L Short ◽  
Megan J Wallace ◽  
Stuart B Hooper ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Preterm Birth ◽  
Transcriptome Sequencing ◽  
Target Genes ◽  
Fetal Lung ◽  
Lung Fibroblasts ◽  
Matrix Remodeling ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

Abstract Preterm birth is characterized by severe lung immaturity that is frequently treated antenatally or postnatally with the synthetic steroid betamethasone. The underlying cellular targets and pathways stimulated by betamethasone in the fetal lung are poorly defined. In this study, betamethasone was compared with corticosterone in steroid-treated primary cultures of fetal rat lung fibroblasts stimulated for 6 hours and analyzed by whole-cell transcriptome sequencing and glucocorticoid (GC) receptor (GR) chromatin immunoprecipitation sequencing (ChIP-Seq) analysis. Strikingly, betamethasone stimulated a much stronger transcriptional response compared with corticosterone for both induced and repressed genes. A total of 483 genes were significantly stimulated by betamethasone or corticosterone, with 476 stimulated by both steroids, indicating a strong overlap in regulation. Changes in mRNA levels were confirmed by quantitative PCR for eight induced and repressed target genes. Pathway analysis identified cell proliferation and cytoskeletal/cell matrix remodeling pathways as key processes regulated by both steroids. One target, transglutaminase 2 (Tgm2), was localized to fetal lung mesenchymal cells. Tgm2 mRNA and protein levels were strongly increased in fibroblasts by both steroids. Whole-genome GR ChIP-Seq analysis with betamethasone identified GC response element–binding sites close to the previously characterized GR target genes Per1, Dusp1, Fkbp5, and Sgk1 and near the genes identified by transcriptome sequencing encoding Crispld2, Tgm2, Hif3α, and Kdr, defining direct genomic induction of expression in fetal lung fibroblasts via the GR. These results demonstrate that betamethasone stimulates specific genes and cellular pathways controlling cell proliferation and extracellular matrix remodeling in lung mesenchymal fibroblasts, providing a basis for betamethasone’s treatment efficacy in preterm birth.

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