scholarly journals Assessment of sample processing methods for stable isotope analysis of aquatic food webs

2008 ◽  
Vol 20 (1) ◽  
pp. 13-20
Author(s):  
ZENG Qingfei ◽  
◽  
KONG Fanxiang ◽  
ZHANG Enlou ◽  
QIAN Shanqin
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Sample Processing ◽  
Processing Methods ◽  
Aquatic Food Webs ◽  
Aquatic Food

scholarly journals Sample preservation and pre-treatment in stable isotope analysis: Implications for the study of aquatic food webs

2020 ◽  
Author(s):  
Marc Jürgen Silberberger ◽  
Katarzyna Koziorowska-Makuch ◽  
Karol Kuliński ◽  
Monika Kędra
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Mixing Models ◽  
Sample Preservation ◽  
Aquatic Food Webs ◽  
Bayesian Mixing Models ◽  
Aquatic Food ◽  
Pre Treatment

Abstract. Stable isotope analysis has become one of the most widely used techniques in ecology. However, uncertainties about the effects of sample preservation and pre-treatment on the ecological interpretation of stable isotope data and especially on Bayesian stable isotope mixing models remain. Here, Bayesian mixing models were used to study how three different preservation methods (drying, freezing, formalin) and two pre-treatments (acidification, lipid removal) affect the estimation of diet composition for two benthic invertebrate species (Limecola balthica, Crangon crangon). Furthermore, commonly used mathematical lipid normalization and formalin correction were applied to check if they improve the model results. Preservation effects were strong on model outcomes for frozen as well as formalin preserved L. balthica samples, but not for C. crangon. Pre-treatment effects varied with species and preservation method and neither lipid normalization nor mathematical formalin correction consistently resulted in improved model outcomes. Our analysis highlights that particularly small changes in δ15N introduced by different preservation and pre-treatments display a so far unrecognized source of error in stable isotope studies. We conclude that mathematical correction of stable isotopes data should be avoided for Bayesian mixing models and that previously unaddressed effects of sample preservation (especially those arising from preservation by freezing) have potentially biased our understanding of the utilization of organic matter in aquatic food webs.

Current methods and future directions in avian diet analysis

The Auk ◽  
2021 ◽  
Author(s):  
Brandon D Hoenig ◽  
Allison M Snider ◽  
Anna M Forsman ◽  
Keith A Hobson ◽  
Steven C Latta ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Stable Isotope Analysis ◽  
Dietary Habits ◽  
Isotope Analysis ◽  
Diet Analysis ◽  
Unified Framework ◽  
Future Directions ◽  
Laboratory Techniques ◽  
Prey Identification

Abstract Identifying the composition of avian diets is a critical step in characterizing the roles of birds within ecosystems. However, because birds are a diverse taxonomic group with equally diverse dietary habits, gaining an accurate and thorough understanding of avian diet can be difficult. In addition to overcoming the inherent difficulties of studying birds, the field is advancing rapidly, and researchers are challenged with a myriad of methods to study avian diet, a task that has only become more difficult with the introduction of laboratory techniques to dietary studies. Because methodology drives inference, it is important that researchers are aware of the capabilities and limitations of each method to ensure the results of their study are interpreted correctly. However, few reviews exist which detail each of the traditional and laboratory techniques used in dietary studies, with even fewer framing these methods through a bird-specific lens. Here, we discuss the strengths and limitations of morphological prey identification, DNA-based techniques, stable isotope analysis, and the tracing of dietary biomolecules throughout food webs. We identify areas of improvement for each method, provide instances in which the combination of techniques can yield the most comprehensive findings, introduce potential avenues for combining results from each technique within a unified framework, and present recommendations for the future focus of avian dietary research.

Stable isotope analysis of food webs in wetland areas of Lake Balaton, Hungary abb;5

1999 ◽  
Vol 146 (1) ◽  
pp. 117-128 ◽  
Author(s):  
István Tátrai ◽  
Kálmán Mátyás ◽  
János Korponai ◽  
Gábor Paulovits ◽  
Piroska Pomogyi ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Lake Balaton

scholarly journals Different transfer pathways of an organochlorine pesticide across marine tropical food webs assessed with stable isotope analysis

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0191335 ◽  
Author(s):  
Charlotte R. Dromard ◽  
Yolande Bouchon-Navaro ◽  
Sébastien Cordonnier ◽  
Mathilde Guéné ◽  
Mireille Harmelin-Vivien ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Organochlorine Pesticide ◽  
Stable Isotope Analysis ◽  
Isotope Analysis

An assessment of sample processing methods for stable isotope analyses of marine food webs

2006 ◽  
Vol 336 (2) ◽  
pp. 254-261 ◽  
Author(s):  
Sirka Carabel ◽  
Enrique Godínez-Domínguez ◽  
Patricia Verísimo ◽  
Luís Fernández ◽  
Juan Freire
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Sample Processing ◽  
Processing Methods ◽  
Stable Isotope Analyses ◽  
Marine Food Webs ◽  
Marine Food ◽  
Isotope Analyses

Effects of terrestrial input on macrobenthic food webs of coastal sea are detected by stable isotope analysis in Gaeta Gulf

2015 ◽  
Vol 154 ◽  
pp. 158-168 ◽  
Author(s):  
Giulio Careddu ◽  
Maria Letizia Costantini ◽  
Edoardo Calizza ◽  
Pasquale Carlino ◽  
Flavia Bentivoglio ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Coastal Sea ◽  
Terrestrial Input

Gut content and stable isotope analysis of tadpoles in floodplain wetlands

2018 ◽  
Vol 66 (4) ◽  
pp. 261 ◽  
Author(s):  
J. F. Ocock ◽  
K. J. Brandis ◽  
B. J. Wolfenden ◽  
K. M. Jenkins ◽  
S. Wassens
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Management Strategies ◽  
Isotope Analysis ◽  
Food Sources ◽  
Gut Contents ◽  
Floodplain Wetlands ◽  
Gut Content ◽  
Larval Amphibians ◽  
Aquatic Food

Larval amphibians (tadpoles) are an important link in aquatic food webs, as they can be highly abundant consumers and prey for a wide variety of predators. Most tadpoles are considered omnivores, predominately grazing on algae, detritus and macrophytes, though recent work has identified greater plasticity and breadth in diet than previously considered. We used gut content and stable isotope analysis (SIA) in a baseline study to determine the important dietary items (ingested material) and food sources (assimilated material) for tadpoles of two abundant generalist frog species in regulated floodplain wetlands of the Murrumbidgee River, south-east Australia. We identified a wide variety of dietary items in the gut contents, including whole microcrustaceans, filamentous algae and macrophytes. The composition of several ingested food items was correlated with their availability in each wetland. However, SIA identified biofilm as the food source most consistently assimilated across several wetlands, though microcrustaceans and algae contributed when abundant. Biofilm is likely the most important basal food item for tadpoles in floodplain wetlands because it is ubiquitous and has a high nutritional quality. Identifying important food sources is a crucial step towards developing management strategies for promoting tadpole recruitment in regulated wetlands.

Stable isotope analysis confirms substantial differences between subtropical and temperate shallow lake food webs

Hydrobiologia ◽  
2016 ◽  
Vol 784 (1) ◽  
pp. 111-123 ◽  
Author(s):  
Carlos Iglesias ◽  
Mariana Meerhoff ◽  
Liselotte S. Johansson ◽  
Ivan González-Bergonzoni ◽  
Néstor Mazzeo ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Food Webs ◽  
Stable Isotope Analysis ◽  
Shallow Lake ◽  
Isotope Analysis
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