scholarly journals 'Complementary' immune evasion by oral pathogen Porphyromonas gingivalis.

2010 ◽  
Author(s):  
Jennifer Krauss
Keyword(s):  
Porphyromonas Gingivalis ◽  
Immune Evasion ◽  
Oral Pathogen

scholarly journals Th1 biased response to a novel Porphyromonas gingivalis protein aggravates bone resorption caused by this oral pathogen

2008 ◽  
Vol 10 (6) ◽  
pp. 664-672 ◽  
Author(s):  
Onir Leshem ◽  
Suely S. Kashino ◽  
Reginaldo B. Gonçalves ◽  
Noriyuki Suzuki ◽  
Masao Onodera ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Porphyromonas Gingivalis ◽  
Oral Pathogen

scholarly journals Mechanisms of vascular damage by systemic dissemination of the oral pathogen Porphyromonas gingivalis

FEBS Journal ◽  
2020 ◽  
Author(s):  
Cher Farrugia ◽  
Graham P. Stafford ◽  
Jan Potempa ◽  
Robert N. Wilkinson ◽  
Yan Chen ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Vascular Damage ◽  
Oral Pathogen ◽  
Systemic Dissemination

Tobacco tars promote the growth of Porphyromonas gingivalis an anaerobic oral pathogen

Bone ◽  
1995 ◽  
Vol 16 (3) ◽  
pp. 402
Author(s):  
M. Al-Bahrani ◽  
J. Fletcher ◽  
M. Wilson ◽  
M. Harris ◽  
S.J. Hodges
Keyword(s):  
Porphyromonas Gingivalis ◽  
Oral Pathogen

Biochemical characterization of the γ-carbonic anhydrase from the oral pathogen Porphyromonas gingivalis, PgiCA

2013 ◽  
Vol 29 (4) ◽  
pp. 532-537 ◽  
Author(s):  
Sonia Del Prete ◽  
Viviana De Luca ◽  
Daniela Vullo ◽  
Andrea Scozzafava ◽  
Vincenzo Carginale ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Porphyromonas Gingivalis ◽  
Biochemical Characterization ◽  
Oral Pathogen

scholarly journals Conjugal Transfer of Chromosomal DNA Contributes to Genetic Variation in the Oral Pathogen Porphyromonas gingivalis

2007 ◽  
Vol 189 (17) ◽  
pp. 6382-6388 ◽  
Author(s):  
Gena D. Tribble ◽  
Gwyneth J. Lamont ◽  
Ann Progulske-Fox ◽  
Richard J. Lamont
Keyword(s):  
Genetic Variation ◽  
Porphyromonas Gingivalis ◽  
Significant Degree ◽  
Dna Transfer ◽  
Chromosomal Dna ◽  
Adaptation Mechanism ◽  
Oral Pathogen ◽  
Type Iv ◽  
Molecular Events

ABSTRACT Porphyromonas gingivalis is a major oral pathogen that contributes to the development of periodontal disease. There is a significant degree of genetic variation among strains of P. gingivalis, and the population structure has been predicted to be panmictic, indicating that horizontal DNA transfer and recombination between strains are likely. The molecular events underlying this genetic exchange are not understood, although a putative type IV secretion system is present in the genome sequence of strain W83, implying that DNA conjugation may be responsible for genetic transfer in these bacteria. In this study, we provide in vitro evidence for the horizontal transfer of DNA using plasmid- and chromosome-based assays. In the plasmid assays, Bacteroides-derived shuttle vectors were tested for transfer from P. gingivalis strains into Escherichia coli. Of the eight strains tested, five were able to transfer DNA into E. coli by a mechanism most consistent with conjugation. Additionally, strains W83 and 33277 tested positive for the transfer of chromosomally integrated antibiotic resistance markers. Ten chimeras resulting from the chromosomal transfer assay were further analyzed by Southern hybridization and were shown to have exchanged DNA fragments of between 1.1 and 5.6 kb, but the overall strain identity remained intact. Chimeras showed phenotypic changes in the ability to accrete into biofilms, implying that DNA transfer events are sufficient to generate measurable changes in complex behaviors. This ability to transfer chromosomal DNA between strains may be an adaptation mechanism in the complex environment of the host oral cavity.

scholarly journals Oral Pathogen Porphyromonas gingivalis Can Escape Phagocytosis of Mammalian Macrophages

2020 ◽  
Vol 8 (9) ◽  
pp. 1432
Author(s):  
Erik R. Werheim ◽  
Kevin G. Senior ◽  
Carly A. Shaffer ◽  
Giancarlo A. Cuadra
Keyword(s):  
Escherichia Coli ◽  
Porphyromonas Gingivalis ◽  
Microbial Pathogens ◽  
Intracellular Bacteria ◽  
Raw 264.7 ◽  
Phagocytic Cells ◽  
Oral Pathogen ◽  
Human Macrophages ◽  
Macrophage Phagocytosis ◽  
Mouse Macrophages

Macrophages are phagocytic cells that play a key role in host immune response and clearance of microbial pathogens. Porphyromonas gingivalis is an oral pathogen associated with the development of periodontitis. Escape from macrophage phagocytosis was tested by infecting THP-1-derived human macrophages and RAW 264.7 mouse macrophages with strains of P. gingivalis W83 and 33277 as well as Streptococcus gordonii DL1 and Escherichia coli OP50 at MOI = 100. CFU counts for all intracellular bacteria were determined. Then, infected macrophages were cultured in media without antibiotics to allow for escape and escaping bacteria were quantified by CFU counting. P. gingivalis W83 displayed over 60% of the bacterial escape from the total amount of intracellular CFUs, significantly higher compared to all other bacteria strains. In addition, bacterial escape and re-entry were also tested and P. gingivalis W83, once again, showed the highest numbers of CFUs able to exit and re-enter macrophages. Lastly, the function of the PG0717 gene of P. gingivalis W83 was tested on escape but found not related to this activity. Altogether, our results suggest that P. gingivalis W83 is able to significantly avoid macrophage phagocytosis. We propose this ability is likely linked to the chronic nature of periodontitis.

Bismuth drugs tacklePorphyromonas gingivalisand attune cytokine response in human cells

Metallomics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1207-1218 ◽  
Author(s):  
Tianfan Cheng ◽  
Yau-Tsz Lai ◽  
Chuan Wang ◽  
Yi Wang ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Inflammatory Responses ◽  
Human Cells ◽  
Cytokine Response ◽  
Oral Pathogen

Repurposing bismuth drugs against the key oral pathogenPorphyromonas gingivalisin planktonic, biofilm, and intracellular states for reconciling the immuno-inflammatory responses.

scholarly journals A Secreted Bacterial Peptidylarginine Deiminase Can Neutralize Human Innate Immune Defenses

mBio ◽  
2018 ◽  
Vol 9 (5) ◽  
Author(s):  
Tim Stobernack ◽  
Marines du Teil Espina ◽  
Lianne M. Mulder ◽  
Laura M. Palma Medina ◽  
Dillon R. Piebenga ◽  
...  
Keyword(s):  
Immune System ◽  
Immune Responses ◽  
Immune Evasion ◽  
Histone H3 ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Peptidylarginine Deiminase ◽  
Oral Pathogen ◽  
Content Type ◽  
Immune Defenses

ABSTRACTThe keystone oral pathogenPorphyromonas gingivalisis associated with severe periodontitis. Intriguingly, this bacterium is known to secrete large amounts of an enzyme that converts peptidylarginine into citrulline residues. The present study was aimed at identifying possible functions of this citrullinating enzyme, namedPorphyromonaspeptidylarginine deiminase (PPAD), in the periodontal environment. The results show that PPAD is detectable in the gingiva of patients with periodontitis, and that it literally neutralizes human innate immune defenses at three distinct levels, namely bacterial phagocytosis, capture in neutrophil extracellular traps (NETs), and killing by the lysozyme-derived cationic antimicrobial peptide LP9. As shown by mass spectrometry, exposure of neutrophils to PPAD-proficient bacteria reduces the levels of neutrophil proteins involved in phagocytosis and the bactericidal histone H2. Further, PPAD is shown to citrullinate the histone H3, thereby facilitating the bacterial escape from NETs. Last, PPAD is shown to citrullinate LP9, thereby restricting its antimicrobial activity. The importance of PPAD for immune evasion is corroborated in the infection modelGalleria mellonella, which only possesses an innate immune system. Together, the present observations show that PPAD-catalyzed protein citrullination defuses innate immune responses in the oral cavity, and that the citrullinating enzyme ofP. gingivalisrepresents a new type of bacterial immune evasion factor.IMPORTANCEBacterial pathogens do not only succeed in breaking the barriers that protect humans from infection, but they also manage to evade insults from the human immune system. The importance of the present study resides in the fact that protein citrullination is shown to represent a new bacterial mechanism for immune evasion. In particular, the oral pathogenP. gingivalisemploys this mechanism to defuse innate immune responses by secreting a protein-citrullinating enzyme. Of note, this finding impacts not only the global health problem of periodontitis, but it also extends to the prevalent autoimmune disease rheumatoid arthritis, which has been strongly associated with periodontitis, PPAD activity, and loss of tolerance against citrullinated proteins, such as the histone H3.

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